The effects of mono-2-ethylhexyl phathalate, adriamycin and N-ethyl-N-nitrosourea on stage-specific apoptosis and DNA synthesis in the mouse spermatogenesis

Toxicology Letters

Volumn 143, Issue 2, 2003, Pages 163-173

  Suominen, Janne S ^a   Linderborg, Jussi ^a   Nikula, Hannu ^a   Hakovirta, Harri ^a   Parvinen, Martti ^a   Toppari, Jorma ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

Apoptosis; DNA synthesis; Reproductive toxicology; Spermatogenesis; Testis

Indexed keywords

DOXORUBICIN; ETHYLNITROSOUREA; PHTHALIC ACID 2 ETHYLHEXYL MONOESTER; TRITIUM;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL COUNT; CELL GROWTH; CONCENTRATION RESPONSE; DNA SYNTHESIS; GERM CELL; IN VITRO STUDY; INTERMETHOD COMPARISON; MALE; MICROSCOPY; MOUSE; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; REPRODUCTIVE TOXICITY; SEMINIFEROUS TUBULE EPITHELIUM; SPERMATOGENESIS; STATISTICAL ANALYSIS; TESTIS;

EID: 0038732341     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4274(03)00170-X     Document Type: Article

References (37)

1. Albro P.W., Chapin R.E., Corbett J.T., Schroeder J., Phelps J.L. Mono-2-ethylhexyl phthalate, a metabolite of di-2-ethylhexyl phthalate, causally linked to testicular atrophy in rats. Toxicol. Appl. Pharmacol. 100:1989;193-200.
2. Chapin R.E., Gray T.J.B., Phelps J.L., Dutton S.L. The effects of mono-2-ethylhexyl phthalate on rat Sertoli cell-enriched primary cultures. Toxicol. Appl. Pharmacol. 92:1988;467-479.
3. Creasy D.M., Beech L.M., Gray T.J., Butler W.H. The ultrastructural effects of di-n-pentyl phthalate on the testis of the mature rat. Exp. Mol. Pathol. 46:1987;357-371.
4. Favor J. Mechanisms of mutation induction in germ cells of the mouse as assessed by the specific locus test. Mutat. Res. 428:1999;227-236.
5. Favor J., Neuhäuser-Klaus A., Ehling U.H. The frequency of dominant cataract and recessive specific-locus mutations and mutation mosaics in F1 mice derived from post-spermatogonial treatment with ethylnitrosourea. Mutat. Res. 229:1990;105-114.
6. Grasso P., Heindel J.J., Powell C.J., Reichert L.E. Effects of mono-2-ethylhexyl phthalate, a testicular toxicant, on follicle-stimulating hormone binding to membranes from cultured rat Sertoli cells. Biol. Reprod. 48:1993;454-459.
7. Gray T.J., Gangolli S.D. Aspect of the testicular toxicity of phthalate esters. Environ. Health Perspect. 65:1986;229-235.
8. Henriksén K., Hakovirta H., Parvinen M. In situ quantification of stage-specific apoptosis in the rat seminiferous epithelium: effects of short-term experimental cryptorchidism. Int. J. Androl. 18:1995;256-262.
9. Hsiang Y.H., Wu H.Y., Liu L.F. Topoisomerases: novel therapeutic targets in cancer chemotherapy. Biochem. Pharmacol. 37:1988;1801-1802.
10. Iona S., Klinger F.G., Sisti R., Ciccalese R., Nunziata A., De Felizi M. A comparative study of cytotoxic effects of N-ethyl-N-nitrosourea, adriamycin and mono-(2-ethylhexyl)phthalate on mouse primordial germ cells. Cell. Biol. Toxicol. 18:2002;131-145.
11. Jahnukainen K., Hou M., Parvinen M., Eksborg S., Söder O. Stage-specific inhibition of deoxyribonucleic acid synthesis and induction of apoptosis by anthracyclines in cultured rat spermatogenis cells. Biol. Reprod. 63:2000;482-487.
12. Jenkins G.J., Chalesthori M.H., Song H., Parry J.M. Mutation analysis using the restriction site mutation (RSM) assay. Mutat. Res. 405:1998;209-220.
13. Kasahara E., Sato F.E., Miyoshi M., Konaka R., Hiramoto K., Sasaki J., Tokuda M., Nakano Y., Inoue M. Role of oxidative stress in germ cell apoptosis induced by di-2-ethylhexyl phthalate. Biochem. J. 365:2002;849-856.
14. Lähdetie J. Meiotic micronuclei induced by adriamycin in male rats. Mutat. Res. 119:1983;79-82.
15. Li L.H., Jester W.F., Orth J.M. Effects of relatively low levels of mono-2-ethylhexyk phthalate on cocultured Sertoli cells and gonocytes from neonatal rats. Toxicol. Appl. Pharmacol. 153:1998;258-265.
16. Parvinen M. Regulation of the seminiferous epithelium. Endocr. Rev. 3:1982;404-416.
17. Parvinen M., Hecht N.B. Identification of living spermatogenic cells of the mouse by transillumination-phase contrast microscopic technique for in situ analyses of DNA polymerase activities. Histochemistry. 71:1981;567-579.
18. Parvinen L.M., Parvinen M. Biochemical studies of the rat seminiferous epithelial wave: DNA and RNA syntheses and effects of adriamycin. Ann. Biol. Biochem. Biophys. 18:1978;585-594.
19. Parvinen M., Vanha-Perttula T. Identification and enzyme quantification of the stages the seminiferous epithelial wave in the rat. Anat. Rec. 174:1972;435-449.
20. Peck C.C., Odom D.G., Friedman H.I., Albro P.W., Hass J.R., Brady J.T., Jess D.A. Di-2-ethylhexyl phthalate (DEHP) and mono-2-ethylhexyl phthalate (MEHP) accumulation in whole blood and red cell concentrates. Transfusion. 19:1979;137-146.
21. Richburg J.H., Boekelheide K. Mono-2-ethylhexyl phthalate rapidly alters both Sertoli cell vimentin filaments and germ cell apoptosis in young rat testes. Toxicol. Appl. Pharmacol. 137:1996;42-50.
22. Richburg J.H., Nanez A., Gao H. Participation of the fas-signaling system in the initiation of germ cell apoptosis in young rat testes after exposure to mono-2-ethylhexyl phthalate. Toxicol. Appl. Pharmacol. 160:1999;271-278.
23. Richburg J.H., Nanez A., Williams L.R., Embree M., Boekelheide K. Sensitivity of testicular germ cells to toxicant-induced apoptosis in gld mice that express a nonfunctional form of fas ligand. Endocrinology. 141:2000;787-793.
24. Ross W.E. DNA topoisomerases as targets for cancer therapy. Biochem. Pharmacol. 34:1985;4191-4195.
25. Russell L.D., Griswold M. Sertoli cell toxicants. Boekelheide K. The Sertoli Cell. 1993;551-575 Cache River Press, Clearwater, FL.
26. Sawada T., Tamada H., Mori J. Secretion of testosterone and epidermal growth factor in mice with oligozoospermia caused by doxorubicin hydrochloride. Andrologia. 26:1994;151-153.
27. Sharman M., Read W.A., Castle L., Gilbert J. Levels of di-2-ethylhexyl phthalate and total phthalate esters in milk, cream, butter and cheese. Food Addit. Contam. 11:1994;375-385.
28. Shinoda K., Mitsumori K., Yasuhara K., Uneyama C., Onodera H., Hirose M., Uehara M. Doxorubicin induces male germ cell apoptosis in rats. Arch. Toxicol. 73:1999;274-281.
29. Sjöblom T., West A., Lähdetie J. Apoptotic response of spermatogenic cells to the germ cell mutagens etoposide, adriamycin and diepoxybutane. Environ. Mol. Mutag. 31:1998;133-148.
30. Sotomayor R.E., Ehling U.H., Sega G.A. Differential sensitivities of spermatogonial and postspermatogonial cell stages of the mouse to induction of unscheduled DNA synthesis by ethyl- and methyl-nitrosourea. Teratogen. Carcinog. Mutagen. 19:1999;339-351.
31. Tewey K.M., Rowe T.C., Yang L., Halligan B.D., Liu L.F. Adriamycin-induced DNA damage mediated by mammalian DNA topoisomerase II. Science. 226:1984;466-468.
32. Toppari J., Lähdetie J., Härkönen P., Eerola E., Parvinen M. Mutagen effects on rat seminiferous tubules in vitro: induction of meiotic micronuclei by adriamycin. Mutat. Res. 171:1986;149-156.
33. Toppari J., Bishop P.C., Parker J.W., di Zerega G.S. DNA flow cytometric analysis of mouse seminiferous epithelium. Cytometry. 9:1988;456-462.
34. Toppari J., Larsen J.C., Christiansen P., Giwercman A., Grandjean P., Guillette L.J., Jégou B., Jensen T.K., Jouannet P., Keiding N., Leffers H., McLachlan J.A., Meyer O., Müller J., Rajpert-De Meyts E., Scheike T., Sharpe R., Sumpter J., Skakkebaek N.E. Male reproductive health and environmental xenoestrogens. Environ. Health Perspect. 104:(Suppl. 4):1996;741-803.
35. van Zeeland A.A., de Groot A., Neuhäuser-Klaus A. DNA adduct formation in mouse testis by ethylating agents: a comparison with germ-cell mutagenesis. Mutat. Res. 231:1990;55-62.
36. Vesselinovitch S.D., Rao K.V.N., Mihailovich N., Rice J.M., Lombard L.S. Development of broad spectrum of tumours by ethylnitrosourea in mice and the modifying role of age, sex and strain. Cancer Res. 34:1974;2530-2538.
37. Williams J., Foster P.M.D. The production of lactate and pyruvate as sensitive indices of altered rat Sertoli cell function in vitro following the addition of various testicular toxicants. Toxicol. Appl. Pharmacol. 94:1988;160-170.