An upstream AG determines whether a downstream AG is selected during catalytic step II of splicing

Molecular and Cellular Biology

Volumn 21, Issue 5, 2001, Pages 1509-1514

  Chua, K ^a   Reed, R ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CATALYSIS; ENZYME LOCALIZATION; ESTERIFICATION; GENE DUPLICATION; GENETIC MANIPULATION; GENETIC SELECTION; MOLECULAR RECOGNITION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; RECEPTOR BLOCKING; RNA SPLICING; SPLICEOSOME;

CELL NUCLEUS; MODELS, BIOLOGICAL; NUCLEOTIDES; PLASMIDS; RNA PRECURSORS; RNA SPLICE SITES; RNA SPLICING;

ANIMALIA;

EID: 0035133535     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.5.1509-1514.2001     Document Type: Article

References (30)

1. Bimolecular exon ligation by the human spliceosome
2. Splicing of precursors to mRNA by the spliceosomes
3. Evidence for a linear search in bimolecular 3′ splice site AG selection
4. Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly
5. Evidence that U5 snRNP recognizes the 3′ splice site for catalytic step II in mammals
6. The RNA splicing factor hSlu7 is required for correct 3′ splice site choice
7. Allele-specific genetic interactions between Prp8 and RBA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome
8. Unexpected point mutations activate cryptic 3′ splice sites by perturbing a natural secondary structure within a yeast intron
9. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae
10. An essential splicing factor. SLU7, mediates 3′ splice site choice in yeast
11. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing
12. A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction
13. Impairment of yeast pre-mRNA splicing by potential secondary structure-forming sequences near the conserved branchpoint sequence
14. Mechanisms of human beta-globin pre-mRNA splicing
15. Crosslink ing of the U5 snRNP-specific 116-kDa protein to RNA hairpins that block step 2 of splicing
16. The role of branchpoint-3′ splice site spacing and interaction between intron terminal nucleotides in 3′ splice site selection in Saccharomyces cerevisiae
17. Inhibition of msl-2 splicing by Sex-lethal reveals interaction between U2AF35 and the 3′ splice site AG
18. Splicing of precursors to messenger RNAs by the spliceosome
19. A U-rich tract enhances usage of an alternative 3′ splice site in yeast
20. Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center
21. Scanning from an independently specified branch point defines the 3′ splice site of mammalian introns
22. Scanning and competition between AGs are involved in 3′ splice site selection in mammalian introns
23. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA
24. Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3′ splice site selection
25. Prp16p, Slu7p, and Prp8p interact with the 3′ splice site in two distinct stages during the second catalytic step of pre-mRNA splicing
26. The second catalytic step of pre-mRNA splicing
27. Functional recognition of the 3′ splice site AG by the splicing factor U2AF35
28. Site-specific crosslinking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing
29. The conserved dinucleotide AG of the 3′ splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors
30. Both subunits of U2AF recognize the 3′ splice site in Caenorhabditis elegans