Biochemical and genetic evidence for a family of heterotrimeric G-proteins in Trichomonas vaginalis

Molecular and Biochemical Parasitology

Volumn 129, Issue 2, 2003, Pages 179-189

  Hirt, R P ^a   Lal, K ^a   Pinxteren, J ^b   Warwicker, J ^c   Healy, B ^a   Coombs, G H ^d   Field, M C ^e   Embley, T M ^a  

^a DEPARTMENT OF LIFE SCIENCES   (United Kingdom)

^b GHENT UNIVERSITY   (Belgium)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

^d UNIVERSITY OF GLASGOW   (United Kingdom)

^e WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

Alpha subunit; Heterotrimeric G proteins; Immunocytochemistry; Molecular modelling; Parasitic protozoa; Protein purification; Trichomonas vaginalis

Indexed keywords

AMINO ACID; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; HYBRID PROTEIN; PEPTIDE; PROLINE; PROTEIN ANTIBODY; PROTEIN SUBUNIT; PROTOZOAL PROTEIN;

ALPHA CHAIN; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING ASSAY; CONTROLLED STUDY; HETEROLOGOUS EXPRESSION; IMMUNOFLUORESCENCE MICROSCOPY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTOZOAL GENETICS; SEQUENCE HOMOLOGY; TRICHOMONAS VAGINALIS;

MAMMALIA; PROTOZOA; TRICHOMONAS VAGINALIS;

EID: 0038691861     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(03)00122-1     Document Type: Article

References (57)

1. Bourne H.R. GTPases: a family of molecular switches and clocks. Philos. Trans. R. Soc. Lond. B Biol. Sci. 349:1995;283-289.
2. Wilkie T.M., Yokoyama S. Evolution of the G protein alpha subunit multigene family. Soc. Gen. Physiol. Ser. 49:1994;249-270.
3. Morris A.J., Malbon C.C. Physiological regulation of G protein-linked signaling. Physiol. Rev. 79:1999;1373-1430.
4. Leberer E., Thomas D.Y., Whiteway M. Pheromone signalling and polarized morphogenesis in yeast. Curr. Opin. Genet. Dev. 7:1997;59-66.
5. Parent C.A., Devreotes P.N. Molecular genetics of signal transduction in Dictyostelium. Annu. Rev. Biochem. 65:1996;411-440.
6. Jones A.M. G-protein-coupled signaling in Arabidopsis. Curr. Opin. Plant Biol. 5:2002;402-407.
7. Helms J.B. Role of heterotrimeric GTP binding proteins in vesicular protein transport: indications for both classical and alternative G protein cycles. FEBS Lett. 369:1995;84-88.
8. Stow J.L., Heimann K. Vesicle budding on Golgi membranes: regulation by G proteins and myosin motors. Biochim. Biophys. Acta. 14:1998;1-2.
9. Bomsel M., Mostov K. Role of heterotrimeric G proteins in membrane traffic. Mol. Biol. Cell. 3:1992;1317-1328.
10. von Zastrow M., Mostov K. Signal transduction. A new thread in an intricate web. Science. 294:2001;1845-1847.
11. Hollinger S., Hepler J.R. Cellular regulation of RGS proteins: modulators and integrators of G protein signaling. Pharmacol. Rev. 54:2002;527-559.
12. Field M.C., Ali B.R., Field H. GTPases in protozoan parasites: tools for cell biology and chemotherapy. Parasitol. Today. 15:1999;365-371.
13. Harnett W., Harnett M.M. Heterotrimeric guanine nucleotide-binding proteins in eukaryotic parasites. Parasitol. Today. 14:1998;27-31.
14. New D.C., Wong J.T. The evidence for G-protein-coupled receptors and heterotrimeric G proteins in protozoa and ancestral metazoa. Biol. Signals Recept. 7:1998;98-108.
15. Petrin D., Delgaty K., Bhatt R., Garber G. Clinical and microbiological aspects of Trichomonas vaginalis. Clin. Microbiol. Rev. 11:1998;300-317.
16. Alderete J.F., Lehker M.W., Arroyo R. The mechanisms and molecules involved in cytoadherence and pathogenesis of Trichomonas vaginalis. Parasitol. Today. 11:1995;70-74.
17. Crouch M.L., Alderete J.F. Trichomonas vaginalis interactions with fibronectin and laminin. Microbiology. 145:1999;2835-2843.
18. Fiori P.L., Rappelli P., Addis M.F. The flagellated parasite Trichomonas vaginalis: new insights into cytopathogenicity mechanisms. Microbes Infect. 1:1999;149-156.
19. McKie A.E., Edlind T., Walker J., Mottram J.C., Coombs G.H. The primitive protozoon Trichomonas vaginalis contains two methionine gamma-lyase genes that encode members of the gamma-family of pyridoxal 5′-phosphate-dependent enzymes. J. Biol. Chem. 273:1998;5549-5556.
20. Diamond L. The establishment of various trichomonads of animals and man in axenic culture. J. Parasitol. 43:1957;488-490.
21. Lockwood B.C., North M.J., Coombs G.H. Trichomonas vaginalis, Tritrichomonas foetus, and Trichomitus batrachorum: comparative proteolytic activity. Exp. Parasitol. 58:1984;245-253.
22. Altschul S.F., Madden T.L., Schäffer A.A.et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
23. Letunic I., Goodstadt L., Dickens N.J.et al. Recent improvements to the SMART domain-based sequence annotation resource. Nucleic Acids Res. 30:2002;242-244.
24. Bateman A., Birney E., Cerruti L.et al. The Pfam protein families database. Nucleic Acids Res. 30:2002;276-280.
25. Falquet L., Pagni M., Bucher P.et al. The PROSITE database, its status in 2002. Nucleic Acids Res. 30:2002;235-238.
26. Lambright D.G., Sondek J., Bohm A.et al. The 2.0 A crystal structure of a heterotrimeric G protein. Nature. 379:1996;311-319.
27. Posner B.A., Mixon M.B., Wall M.A., Sprang S.R., Gilman A.G. The A326S mutant of Gialpha1 as an approximation of the receptor-bound state. J. Biol. Chem. 273:1998;21752-21758.
28. Wall M.A., Coleman D.E., Lee E.et al. The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2. Cell. 83:1995;1047-1058.
29. Sali A., Blundell T.L. Comparative protein modelling by satisfaction of spatial restraints. J. Mol. Biol. 234:1993;779-815.
30. Guex N., Peitsch M.C. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modelling. Electrophoresis. 18:1997;2714-2723.
31. Mumby S.M., Gilman A.G. Synthetic peptide antisera with determined specificity for G protein alpha or beta subunits. Methods Enzymol. 195:1991;215-233.
32. Lockwood B.C., North M.J., Coombs G.H. The release of hydrolases from Trichomonas vaginalis and Tritrichomonas foetus. Mol. Biochem. Parasitol. 30:1988;135-142.
33. Pinxteren J.A., O'Sullivan A.J., Tatham P.E., Gomperts B.D. Regulation of exocytosis from rat peritoneal mast cells by G protein beta gamma-subunits. EMBO J. 17:1998;6210-6218.
34. Sternweis PC, Pang IH. Preparation of G-proteins and their subunits. In: Hulme EC, editor. Receptor-effector coupling: a practical approach. New York: IRL Press/Oxford University Press; 1990. p. 1-30.
35. Liston D.R., Johnson P.J. Gene transcription in Trichomonas vaginalis. Parasitol. Today. 14:1998;261-265.
36. McInerney J.O. Codon usage patterns in Trichomonas vaginalis. Eur. J. Protistol. 33:1997;266-273.
37. Espinosa N., Hernandez R., Lopez-Griego L., Arroyo R., Lopez-Villasenor I. Differences between coding and non-coding regions in the Trichomonas vaginalis genome: an actin gene as a locus model(1). Acta Trop. 78:2001;147-154.
38. Wu L., Gaskins C., Zhou K., Firtel R.A., Devreotes P.N. Cloning and targeted mutations of G alpha 7 and G alpha 8, two developmentally regulated G protein alpha-subunit genes in Dictyostelium. Mol. Biol. Cell. 5:1994;691-702.
39. Lee Y.R., Assmann S.M. Arabidopsis thaliana 'extra-large GTP-binding protein' (AtXLG1): a new class of G-protein. Plant Mol. Biol. 40:1999;55-64.
40. Knol J.C., van der Slik A.R., van Kesteren E.R.et al. A novel G protein alpha subunit containing atypical guanine nucleotide-binding domains is differentially expressed in a molluscan nervous system. J. Biol. Chem. 270:1995;18804-18818.
41. Jansen G., Thijssen K.L., Werner P.et al. The complete family of genes encoding G proteins of Caenorhabditis elegans. Nat. Genet. 21:1999;414-419.
42. Bourne H.R., Sanders D.A., McCormick F. The GTPase superfamily: conserved structure and molecular mechanisms. Nature. 349:1991;117-127.
43. Sprang S.R. G protein mechanisms: insights from structural analysis. Annu. Rev. Biochem. 66:1997;639-678.
44. Kjeldgaard M., Nyborg J., Clark B.F. The GTP binding motif: variations on a theme. FASEB J. 10:1996;1347-1368.
45. Ma H., Yanofsky M.F., Meyerowitz E.M. Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A. 87:1990;3821-3825.
46. Bourne H.R., Sanders D.A., McCormick F. The GTPase superfamily: a conserved switch for diverse cell functions. Nature. 348:1990;125-132.
47. Wall M.A., Posner B.A., Sprang S.R. Structural basis of activity and subunit recognition in G protein heterotrimers. Structure. 6:1998;1169-1183.
48. Kehlenbach R.H., Matthey J., Huttner W.B. XL alpha s is a new type of G protein. Nature. 372:1994;804-809.
49. Ugur O., Jones T.L. A proline-rich region and nearby cysteine residues target XLalphas to the Golgi complex region. Mol. Biol. Cell. 11:2000;1421-1432.
50. Kay B.K., Williamson M.P., Sudol M. The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains. FASEB J. 14:2000;231-241.
51. Wedegaertner P.B. Lipid modifications and membrane targeting of G alpha. Biol. Signals Recept. 7:1998;125-135.
52. Goedert M., Baur C.P., Ahringer J.et al. PTL-1, a microtubule-associated protein with tau-like repeats from the nematode Caenorhabditis elegans. J. Cell Sci. 109:1996;2661-2672.
53. Benchimol M., Ribeiro K.C., Mariante R.M., Alderete J.F. Structure and division of the Golgi complex in Trichomonas vaginalis and Tritrichomonas foetus. Eur. J. Cell Biol. 80:2001;593-607.
54. Michaelson D., Ahearn I., Bergo M., Young S., Philips M. Membrane trafficking of heterotrimeric G proteins via the endoplasmic reticulum and Golgi. Mol. Biol. Cell. 13:2002;3294-3302.
55. Hughson EJ, Hirt RP. Assessment of cell polarity. In: Shaw AJ, editor. Cell culture models of epithelial tissues: a practical approach. Oxford: IRL Press/Oxford University Press; 1996. p. 37-66.
56. Blom N., Gammeltoft S., Brunak S. Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J. Mol. Biol. 294:1999;1351-1362.
57. Montmayeur J.P., Borrelli E. Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region. Science. 263:1994;95-98.