Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 285, Issue 1 29-1, 2003, Pages

  Von der Hardt, Katharina ^a   Kandler, Michael Andreas ^a   Fink, Ludger ^b   Schoof, Ellen ^a   Dötsch, Jörg ^a   Bohle, Rainer Maria ^b   Rascher, Wolfgang ^a  

^a UNIVERSITY HOSPITAL ERLANGEN   (Germany)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Aerosolized perfluorocarbon; Interleukin 8; Polymerase chain reaction; Surfactant depleted piglets

Indexed keywords

ANTIINFLAMMATORY AGENT; FLUOROCARBON; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; INTERCELLULAR ADHESION MOLECULE 1; INTERLEUKIN 8; MESSENGER RNA; SURFACTANT;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIINFLAMMATORY ACTIVITY; ARTICLE; ARTIFICIAL VENTILATION; CELL TYPE; CONTROLLED STUDY; DISSECTION; ENDOTHELIUM CELL; EPITHELIUM CELL; FUNCTIONAL RESIDUAL CAPACITY; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; INFLAMMATION; INTERMITTENT MANDATORY VENTILATION; LASER; LUNG ALVEOLUS CELL; LUNG ALVEOLUS MACROPHAGE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SWINE; VASCULAR SMOOTH MUSCLE;

EID: 0038676962     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.00198.2002     Document Type: Article

References (29)

1. Barry BE, Geyer RP, and Brain JD. Pulmonary effects of blood replacement with a perfluorochemical emulsion followed by exposure to 85% oxygen or air. Am Rev Respir Dis 138: 435-444, 1988.
2. Betsuyaku T, Griffin GL, Watson MA, and Senior RM. Laser capture microdissection and real-time reverse transcriptase/polymerase chain reaction of bronchiolar epithelium after bleomycin. Am J Respir Cell Mol Biol 25: 278-284, 2001.
3. Bohle RM, Hartmann E, Kinfe T, Ermert L, Seeger W, and Fink L. Cell type-specific mRNA quantitation in non-neoplastic tissues after laser-assisted cell picking. Pathobiology 68: 191-195, 2000.
4. Colton DM, Till GO, Johnson KJ, Dean SB, Bartlett RH, and Hirschl RB. Neutrophil accumulation is reduced during partial liquid ventilation. Crit Care Med 26: 1716-1724, 1998.
5. Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KA, Stein H, and Mason DY. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32: 219-229, 1984.
6. Cotran RS and Mayadas-Norton T. Endothelial adhesion molecules in health and disease. Pathol Biol (Paris) 46: 164-170, 1998.
7. Croce MA, Fabian TC, Patton JH Jr, Melton SM, Moore M, and Trenthem LL. Partial liquid ventilation decreases the inflammatory response in the alveolar environment of trauma patients. J Trauma 45: 273-280, 1998.
8. Dötsch J, Nüsken KD, Knerr I, Kirschbaum M, Repp R, and Rascher W. Leptin and neuropeptide Y gene expression in human placenta: ontogeny and evidence for similarities to hypothalamic regulation. J Clin Endocrinol Metab 84: 2755-2758, 1999.
9. Fernandez R, Sarma V, Younkin E, Hirschl RB, Ward PA, and Younger JG. Exposure to perflubron is associated with decreased Syk phosphorylation in human neutrophils. J Appl Physiol 91: 1941-1947, 2001.
10. Fink L, Kinfe T, Seeger W, Ermert L, Kummer W, and Bohle RM. Immunostaining for cell picking and real-time mRNA quantitation. Am J Pathol 157: 1459-1466, 2000.
11. Fink L, Kinfe T, Stein MM, Ermert L, Hänze J, Kummer W, Seeger W, and Bohle RM. Immunostaining and laser-assisted cell picking for mRNA analysis. Lab Invest 80: 327-333, 2000.
12. Fink L, Seeger W, Ermert L, Hanze J, Stahl U, Grimminger F, Kummer W, and Bohle RM. Real-time quantitative RT-PCR after laser-assisted cell picking. Nat Med 4: 1329-1333, 1998.
13. Heid CA, Stevens J, Livak KJ, and Williams PM. Real time quantitative PCR. Genome Res 6: 986-994, 1996.
14. Kandler MA, von der Hardt K, Schoof E, Dötsch J, and Rascher W. Persistent improvement of gas exchange and lung mechanics by aerosolized perfluorocarbon. Am J Respir Crit Care Med 164: 31-35, 2001.
15. Leach CL, Fuhrman BP, Morin FC III, and Rath MG. Perfluorocarbon-associated gas exchange (partial liquid ventilation) in respiratory distress syndrome: a prospective, randomized, controlled study. Crit Care Med 21: 1270-1278, 1993.
16. Leach CL, Greenspan JS, Rubenstein SD, Shaffer TH, Wolfson MR, Jackson JC, DeLemos R, and Fuhrman BP. Partial liquid ventilation with perflubron in premature infants with severe respiratory distress syndrome. The LiquiVent Study Group. N Engl J Med 335: 761-767, 1996.
17. MacIntyre N, Andjuval S, and Baran G. Aerosol deposition from an intra-airway aerosol. Eur Respir J 9: P963, 1996.
18. Nader ND, Knight PR, Davidson BA, Safaee SS, and Steinhorn DM. Systemic perfluorocarbons suppress the acute lung inflammation after gastric acid aspiration in rats. Anesth Analg 90: 356-361, 2000.
19. Ohh M, Smith CA, Carpenito C, and Takei F. Regulation of intercellular adhesion molecule-1 gene expression involves multiple mRNA stabilization mechanisms: effects of interferon-γ and phorbol myristate acetate. Blood 84: 2632-2639, 1994.
20. Overbeck MC, Pranikoff T, Yadao CM, and Hirschl RB. Efficacy of perfluorocarbon partial liquid ventilation in a large animal model of acute respiratory failure. Crit Care Med 24: 1208-1214, 1996.
21. Özdemir A, Brown MA, and Morgan WJ. Markers and mediators of inflammation in neonatal lung disease. Pediatr Pulmonol 23: 292-306, 1997.
22. Purucker E and Lutz J. Effect of hyperbaric oxygen treatment and perfluorochemical administration on glutathione status of the lung. Adv Exp Med Biol 317: 131-136, 1992.
23. Quintel M, Heine M, Hirschl RB, Tillmanns R, and Wessendorf V. Effects of partial liquid ventilation on lung injury in a model of acute respiratory failure: a histologic and morphometric analysis. Crit Care Med 26: 833-843, 1998.
24. Rotta AT and Steinhorn DM. Partial liquid ventilation reduces pulmonary neutrophil accumulation in an experimental model of systemic endotoxemia and acute lung injury. Crit Care Med 26: 1707-1715, 1998.
25. Shaffer TH, Wolfson MR, and Clark LC Jr. Liquid ventilation. Pediatr Pulmonol 14: 102-109, 1992.
26. Smith TM, Steinhorn DM, Thusu K, Fuhrman BP, and Dandona P. A liquid perfluorochemical decreases the in vitro production of reactive oxygen species by alveolar macrophages. Crit Care Med 23: 1533-1539, 1995.
27. Speer CP. Inflammatory mechanisms in neonatal chronic lung disease. Eur J Pediatr 158 Suppl 1: S18-S22, 1999.
28. Thomassen MJ, Buhrow LT, and Wiedemann HP. Perflubron decreases inflammatory cytokine production by human alveolar macrophages. Crit Care Med 25: 2045-2047, 1997.
29. Von der Hardt K, Schoof E, Kandler MA, Dötsch J, and Rascher W. Aerosolized perfluorocarbon suppresses early pulmonary inflammatory response in a surfactant-depleted piglet model. Pediatr Res 51: 177-182, 2002.