Apoptotic cell death and function of cryopreserved porcine hepatocytes in a bioartificial liver

Cell Transplantation

Volumn 12, Issue 2, 2003, Pages 109-121

  Matsushita, Takakazu ^a   Yagi, Toshikazu ^a   Hardin, Joseph A ^a   Cragun, Jennifer D ^a   Crow, Frank W ^a   Bergen III, H Robert ^a   Gores, Gregory J ^a   Nyberg, Scott L ^a  

^a MAYO CLINIC   (United States)

Author keywords

Apoptosis; Bioartificial liver; Cryopreservation; Hepatocyte

Indexed keywords

ALBUMIN; CASPASE INHIBITOR; CYTOCHROME C; DIAZEPAM; IDN 1965; LIQUID NITROGEN; UNCLASSIFIED DRUG; UREA;

ANIMAL CELL; APOPTOSIS; ARTICLE; ARTIFICIAL LIVER; BIOARTIFICIAL ORGAN; CELL FUNCTION; CELL ISOLATION; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; LIVER CELL; LIVER CELL CULTURE; LIVER NECROSIS; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; THAWING;

ANIMALIA; SUIDAE;

EID: 0038623548     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/000000003108746696     Document Type: Article

References (35)

1. Adjei. P.; Kaufmann, S.; Mao, W-Y.; Gores, G. Selective induction of apoptosis in Hep 3B cells by topoisomerase I inhibitors: Evidence for a protease-dependent pathway that does not activate cysteine protease P32. J. Clin. Invest. 98:2588-2596; 1996.
2. Albrecht, J.; Jones, E. Hepatic encephalopathy: Molecular mechanisms underlying the clinical syndrome. J. Neurol. Sci. 170:138-146; 1999.
3. Ansari, B.; Coates, P. In situ end-labeling detects DNA strand breaks in apoptosis and other physiological and pathological states. J. Pathol. 170:1; 1993.
4. Frisch, S.; Ruoslahti, E. Integrins and anoikis. Curr. Opin. Cell Biol. 9:701-706; 1997.
5. Frisch, Sm.; Screaton, R. Anoikis mechanisms. Curr. Opin. Cell Biol. 13:555-562; 2001.
6. Gerlach, J.; Brombacher, J.; Smith, M.; Neuhaus, P. High yield hepatocyte isolation from pig livers for investigation of hybrid liver support systems: Influence of collagenase concentration and body weight. J. Surg. Res. 62:85-89; 1996.
7. Gorczyca, W.; Tuziak, T.; Kram, A.; Melamed, M.; Darzynkiewicz, Z. Detection of apoptosis-associated DNA strand breaks in fine-needle aspiration biopsies by in situ end labeling of fragmented DNA. Cytometry 15:169-175; 1994.
8. Groskreutz, J.; Bronk, S.; Gores, G. Ruthenium red delays the onset of cell death during oxidative stress of rat hepatocytes. Gastroenterology 102:1030-1038; 1992.
9. Guillouzo, A.; Rialland, L.; Fautrel, A.; Guyomard, C. Survival and function of isolated hepatocytes after cryopreservation. Chem. Biol. Interact. 121:7-16; 1999.
10. Hansen, T.; Borlak, J.; Bader, A. Cytochrome P450 enzyme activity and protein expression in primary porcine enterocyte and hepatocyte cultures. Xenobiotica 30:27-46; 2000.
11. Hermes-Lima, M. How do Ca2+ and 5-aminolevulinic acid-derived oxyradicals promote injury to isolated mitochondria? Free Radic. Biol. Med. 19:381-390; 1995.
12. Hoglen, N.; Hirakawa, B.; Fisher, C.; Weeks, S.; Srinivasan, A.; Wong, A.; et al. Characterization of the caspase inhibitor IDN-1965 in a model of apoptosis-associated liver injury. J. Pharmacol. Exp. Ther. 297:811-818; 2001.
13. Jauregui, H.; Hayner, N.; Driscoll, J.; Williams-Holland, R.; Lipsky, M.; Galletti, P. Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes-freshly isolated cells, cell suspension, and primary monolayer cultures. In Vitro 81:1100-1110; 1981.
14. Juan, G.; Cavazzoni, M.; Saez, G.; O'connor, J-E. A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry. Cytometry 15:335-342; 1994.
15. Kohli, V.; Selzner, M.; Madden, J.; Bentley, R.; Clavien, P-A. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemia-reperfusion injury in the rat liver. Transplantation 67:1099-1105; 1999.
16. Leist, M.; Volbracht, C.; Fava, E.; Nicotera, P. 1-Methyl4-phenylpyridinium induces autocrine excitotoxicity, protease activation, and neuronal apoptosis. Mol. Pharmacol. 54:789-801; 1998.
17. Lemaster, J.; Nieminen, a-L.; Qian, T.; Trost, L.; Elmore, S.; Nishimura, Y.; et al. The mitochondrial permeability transition in cell death: A common mechanism in necrosis, apoptosis, and autophagy. Biochim. Biophys. Acta 1366: 177-196;1998.
18. Li, P.; Nijhawan, D.; Budihardjo, I.; Srinivasula, S.; Ahmad, M.; Alnemri, E. Cytochrome c and dATP-dependent formation of apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-489; 1997.
19. Mcdowell, E.; Trump, B. Histologic fixatives suitable for diagnostic and electron microscopy. Arch. Pathol. Lab. Med. 100:405-414; 1976.
20. Natori, S.; Selzner, M.; Valentino, K.; Fritz, L.; Srinivasan, A.; Clavien, P.; et al. Apoptosis of sinusoidal endothelial cells occurs during liver preservation injury by a caspase-dependent mechanism. Transplantation 68:89-96; 1999.
21. Nyberg, S.; Shatford, R.; Payne, W.; Hu, W-S.; Cerra, F. Staining with fluorescein diacetate correlates with hepatocyte function. Biotech. Histochem. 68:56-63; 1993.
22. Nyberg, S.; Hardin, J.; Matos, L.; Rivera, D.; Misra, S.; Gores, G. Cytoprotective influence of ZVAD-fmk and glycine on gel-entrapped rat hepatocytes in a bioartificial liver. Surgery 127:447-455; 2000.
23. Nyberg, S.; Shatford, R.; Payne, W.; Hu, W.; Cerra, F. Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver. Rat hepatocytes cultured in cylindrical collagen gels. Cytotechnology 10:205-215; 1992.
24. Patzer, J.; Kramer, D.; Mazariegos, G.; Lopez-Solis, R.; Giraldo, M.; Grogan, T.; et al. Clinical safety evaluation of Excorp Medical Inc. bioartificial liver support system (BLSS). ASAIO J. 46:241A; 2000.
25. Petit, P.; Goubern, M.; Diolez, P.; Susin, S.; Zamzami, N.; Kroemer, G. Disruption of the outer mitochondrial membrane as a result of large amplitude swelling: The impact of irreversible permeability transition. FEBS Lett. 426: 111-116; 1998.
26. Puthalakath, H.; Villunger, A.; O'reilly, L.; Beaumont, J.; Coultas, L.; Cheney, R.; et al. Bmf: A proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 293: 1829-1832;2000.
27. Que, F.; Gores, G.; Larusso, N. Development and initial application of an in vitro model of apoptosis in rodent cholangiocytes. Am. J. Physiol. 272:G106-115; 1997.
28. Shatford, R.; Nyberg, S.; Meier, S.; White, J.; Payne, W.; Hu, W-S.; et al. Hepatocyte function in a hollow fiber bioreactor: A potential bioartificial liver. J. Surg. Res. 53: 549-557;1992.
29. Sielaff, T.; Hu, M.; Sridhar, R.; Groehler, K.; Olson, D.; Mann, H.; et al. A technique for porcine hepatocyte harvest and description of differentiated metabolic functions in static culture. Transplantation 59:1459-1463; 1995.
30. Slee, E.; Harte, M.; Kluck, R.; Wolf, B.; Casiano, C.; Newmeyer, D.; et al. Ordering the cytochrome c-initiated caspase cascade: Hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J. Cell Biol. 144:281-292; 1999.
31. Spur, A. A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31-43; 1969.
32. Strain, A.; Neuberger, J. A bioartificial liver - state of the art. Science 295:1005-1009; 2002.
33. Watanabe, F.; Mullon, C-P.; Hewitt, W.; Arkadopoulos, N.; Kahaku, E.; Eguchi, S.; et al. Clinical experience with a bioartifical liver (BAL) in the treatment of severe liver failure: A phase I clinical trial. Ann. Surg. 225:484-494; 1997.
34. Wu, J.; Fritz, L. Irreversible caspase inhibitors: Tools for studying apoptosis. Methods 17:320-328; 1999.
35. Yagi, T.; Hardin, J.; Miyoshi, H.; Gores, G.; Nyberg, S. Caspase inhibition reduces apoptotic death of cryopreserved porcine hepatocytes. Hepatology 33:1432-1440; 2001.