Which sites react first? Functional site distribution and kinetics on solid supports investigated using confocal raman and fluorescence microscopy

Journal of Combinatorial Chemistry

Volumn 5, Issue 1, 2003, Pages 28-32

  Kress, Jürgen ^a   Zanaletti, Riccardo ^a   Rose, Abigail ^a   Frey, Jeremy G ^a   Brocklesby, William S ^a   Ladlow, Mark ^b   Bradley, Mark ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GLASS; RESIN; MICROSPHERE;

ABSORPTION; ARTICLE; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; CONFOCAL MICROSCOPY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; SOLID; BINDING SITE; INSTRUMENTATION; KINETICS; METHODOLOGY; NANOTECHNOLOGY; RAMAN SPECTROMETRY; THREE DIMENSIONAL IMAGING;

BINDING SITES; IMAGING, THREE-DIMENSIONAL; KINETICS; MICROSCOPY, FLUORESCENCE; MICROSPHERES; NANOTECHNOLOGY; RESINS, SYNTHETIC; SPECTRUM ANALYSIS, RAMAN;

EID: 0038540388     PISSN: 15204766     EISSN: None     Source Type: Journal    
DOI: 10.1021/cc020024x     Document Type: Article

References (22)

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20. There has been considerable discussion recently in the literature over the depth resolution than can be expected from a confocal microscope when it is used to probe relatively deeply inside a material. Everall (Everall, N. J. Appl. Spectrosc. 2000, 54, 773) pointed out that the presence of the air/sample interface (in our case, the air/solvent interface for the swollen beads) adversely affects the confocal properties and would lead to a very much worse depth resolution then would occur in air. Subsequent extension of this analysis (Baldwin K. J.; Batchelder, D. N. Appl. Spectrosc. 2001, 55, 517) modified these conclusions, showing the situation was not quite as problematic. However, these analyses do not correspond exactly to our observation conditions. To assess the actual depth resolution in our experiments, we recorded scans both across and down through a swollen spherical bead. A comparison of these two scans indicates a depth resolution of between 10 and 15 μm.
21. There has been considerable discussion recently in the literature over the depth resolution than can be expected from a confocal microscope when it is used to probe relatively deeply inside a material. Everall (Everall, N. J. Appl. Spectrosc. 2000, 54, 773) pointed out that the presence of the air/sample interface (in our case, the air/solvent interface for the swollen beads) adversely affects the confocal properties and would lead to a very much worse depth resolution then would occur in air. Subsequent extension of this analysis (Baldwin K. J.; Batchelder, D. N. Appl. Spectrosc. 2001, 55, 517) modified these conclusions, showing the situation was not quite as problematic. However, these analyses do not correspond exactly to our observation conditions. To assess the actual depth resolution in our experiments, we recorded scans both across and down through a swollen spherical bead. A comparison of these two scans indicates a depth resolution of between 10 and 15 μm.
22. Most of our observations have been made on swollen beads. Typically, the polymer beads swell to twice their diameter in the solvent, and thus, on the order of 7/8 of the bead is now solvent. This automatically ensures that the bead is index-matched with the surrounding solvent (indeed, the swollen beads can be hard to see under the microscope). This removes the refractive focusing effect of the spherical bead surface. In the case of the dry beads, the refraction at the spherical surface bends the focal plane of the microscope considerably as the apparent position is probed away from the center line of the bead, making the interpretation of the observed spatial distribution in a dry bead harder to interpret than in the much more straightforward situation of the swollen beads.