A 'marker switch' approach for targeted mutagenesis of genes in Schizosaccharomyces pombe

Yeast

Volumn 20, Issue 7, 2003, Pages 587-594

  MacIver, Fiona H ^a   Glover, David M ^b   Hagan, Iain M ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b Department of Genetics ^*  (United Kingdom)

Author keywords

Plo1; Polo kinase; Schizosaccharomyces pombe

Indexed keywords

ARTICLE; CONTROLLED STUDY; FUNGAL GENE; FUNGAL GENETICS; FUNGAL STRAIN; GENE FUNCTION; GENE IDENTIFICATION; GENE LOCUS; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; GENETIC MARKER; GENETIC RECOMBINATION; GENETIC TRAIT; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE; TEMPERATURE SENSITIVITY;

BASE SEQUENCE; DNA, FUNGAL; DROSOPHILA PROTEINS; GENES, FUNGAL; GENETIC MARKERS; MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; PLASMIDS; PROTEIN-SERINE-THREONINE KINASES; SCHIZOSACCHAROMYCES; TEMPERATURE;

SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 0038522860     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.983     Document Type: Article

References (25)

1. Bähler J, Steever AB, Wheatley S, et al. 1998a. Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. J Cell Biol 143: 1603-1616.
2. Bähler J, Wu JQ, Longtine MS, et al. 1998b. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951.
3. Baum M, Ngan VK, Clarke L. 1994. The centromeric K-type repeat and the central core are together sufficient to establish a functional Schizosaccharomyces pombe centromere. Mol Biol Cell 5: 747-761.
4. Craven RA, Griffiths DJF, Sheldrick KS, et al. 1998. Vectors for the expression of tagged proteins in Schizosaccharomyces pombe. Gene 221: 59-68.
5. Fromant M, Blanquet S, Plateau P. 1995. Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction. Anal Biochem 224: 347-353.
6. Glover DM, Hagan IM, Tavares A. 1998. Polo kinases: a team that plays throughout mitosis. Genes Dev 12: 3777-3787.
7. Grallert A, Hagan IM. 2002. Schizosaccharomyces pombe NIMA-related kinase Fin1, regulates spindle formation and an affinity of Polo for the SPB. EMBO J 21: 3096-3107.
8. Grallert B, Nurse P, Patterson TE. 1993. A study of integrative transformation in Schizosaccharomyces pombe. Mol Gen Genet 238: 26-32.
9. + gene as a selectable marker. Mol Gen Genet 215: 81-86.
10. Krien M, Bugg S, Palatsides M, et al. 1998. A NIMA homologue promotes chromatin condensation in fission yeast. J Cell Sci 111: 967-976.
11. Longtine M, McKenzie A, Demarini DJ, et al. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961.
12. Marks J, Hyams JS. 1985. Localization of F-actin through the cell-division cycle of Schizosaccharomyces pombe. Eur J Cell Biol 39: 27-32.
13. Moreno S, Klar A, Nurse P. 1991. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194: 795-823.
14. Mulvihill DP, Petersen J, Ohkura H, et al. 1999. Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe. Mol Biol Cell 10: 2771-2785.
15. Nigg EA. 1998. Polo-like kinases: positive regulators of cell division from start to finish. Curr Opin Cell Biol 10: 776-783.
16. Nurse P. 1975. Genetic control of cell size at cell division in yeast. Nature 256: 547-551.
17. 2 cells. Genes Dev 9: 1059-1073.
18. Rose MD, Broach JR. 1991. Cloning genes by complementation. Methods Enzymol 194: 195-230.
19. Takahashi K, Murakami S, Chikashige Y, et al. 1992. A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere. Mol Biol Cell 3: 819-835.
20. Tanaka K, Petersen J, MacIver FH, et al. 2001. The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe. EMBO J 20: 1259-1270.
21. Tasto JK, Carnahan RH, McDonald WH, Gould KL. 2001. Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe. Yeast 18: 657-662.
22. Wach A, Brachat A, Pohlmann R, Philippsen P. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.
23. Weitzer S, Uhlmann F. 2002. Chromosome segregation: playing polo in prophase. Dev Cell 2: 381-382.
24. Winzeler EA, Shoemaker DD, Astronoff A, et al. 1999. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906.
25. Wood V, Gwilliam R, Rajandream MA, et al. 2002. The genome sequence of Schizosaccharomyces pombe. Nature 415: 871-880.