Small interfering RNAs expressed from a Pol III promoter suppress the EWS/Fli-1 transcript in an Ewing sarcoma cell line

Molecular Therapy

Volumn 7, Issue 6, 2003, Pages 811-816

  Dohjima, Taikoh ^a,b   Lee, Nan Sook ^a   Li, Haitang ^a   Ohno, Takatoshi ^b   Rossi, John J ^a  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^b GIFU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Ewing sarcoma; EWS Fli 1; RNA interference; siRNA; U6 promoter

Indexed keywords

DNA DIRECTED RNA POLYMERASE III; MESSENGER RNA; ONCOPROTEIN;

ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CHROMOSOME TRANSLOCATION 11; CONTROLLED STUDY; DOWN REGULATION; ERYTHROLEUKEMIA; EWING SARCOMA; EXPRESSION VECTOR; FUSION GENE; GENE EXPRESSION; GENE TARGETING; GENETIC CODE; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; NEUROECTODERM TUMOR; NONVIRAL GENE DELIVERY SYSTEM; NONVIRAL GENE THERAPY; PROMOTER REGION; QUANTITATIVE ANALYSIS; TUMOR CELL;

EID: 0038489117     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-0016(03)00101-1     Document Type: Article

References (32)

1. Turc-Carel, C., Philip, I., Berger, M. P., Philip, T., and Lanoir, G. (1983). Chromosomal translocation (11;22) in cell lines of Ewing's sarcoma. C. R. Seances Acad. Sci. Ser. 3 296: 1101-1103.
2. Ohno, T., Rao, V. N., and Reddy, E. S. (1993). EWS/Fli-1 chimeric protein is a transcriptional activator. Cancer Res. 53: 5859-5863.
3. Bailly, R. A., et al. (1994). DNA-binding and transcriptional activation properties of the EWS-Fli-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma. Mol. Cell. Biol. 14: 3230-3241.
4. Tanaka, K., Iwakura, T., Harimaya, K., Sato, H., and Iwamoto, Y. (1997). EWS-Fli-1 antisense oligodeoxynucleotide inhibits proliferation of human Ewing's sarcoma and primitive neuroectodermal tumor cells. J. Clin. Invest. 99: 239-247.
5. Hannon, G. J. (2002). RNA interference. Nature 418: 244-251.
6. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998). Potent and specific genetic interference by double-strand RNA in Caenorhabditis elegans. Nature 391: 806-811.
7. Pal-Bhadra, M., Bhadra, U., and Birchler, J. A. (2002). RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila. Mol. Cell 9: 315-327.
8. Elbashir, S. M., Martinez, J., Patkaniowska, A., Lendeckel, W., and Tuschl, T. (2001). Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20: 6877-6888.
9. Bernstein, E., Caudy, A. A., Hammond, S. M., and Hannon, G. J. (2001). Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409: 363-366.
10. Stark, G. R., Kerr, I. M., Williams, B. R., Silverman, R. H., and Schreiber, R. D. (1998). How cells respond to interferons. Annu. Rev. Biochem. 67: 227-264.
11. Clemens, M. J., and Elia, A. (1997). The double-strand RNA-dependent protein kinase PKR: structure and function. J. Interferon Cytokine Res. 17: 503-524.
12. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494-498.
13. Tuschl, T. (2002). Expanding small RNA interference. Nat. Biotechnol. 20: 446-448.
14. Lee, N. S., et al. (2002). Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20: 500-505.
15. Delattre, O., et al. (1992). Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumors. Nature 359: 162-165.
16. Castanotto, D., Li, H., and Rossi, J. J. (2002). Functional siRNA expression from transfected PCR products. RNA 8: 1454-1460.
17. Snyder, D. S., et al. (1993). Ribozyme-mediated inhibition of bcl-abl gene expression in a Philadelphia chromosome-positive cell line. Blood 82: 600-605.
18. Rossi, J. J., and Sarver, N. (1992). Catalytic antisense RNA (ribozymes): their potential and use as anti-HIV-1 therapeutic agents. Adv. Exp. Med. Biol. 312: 95-109.
19. Dauphinot, L., et al. (2001). Analysis of the expression of cell cycle regulators in Ewing cell lines: EWS-Fli-1 modulates p57KIP2 and c-Myc expression. Oncogene 20: 3258-3265.
20. Wilda, M., Fuchs, U., Wossmann, W., and Borkhardt, A. (2002). Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene 21: 5716-5724.
21. Scherr, M., Battmer, K., Winkler, T., Heidenreich, O., Ganser, A., and Eder, M. (2003). Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood 101: 1566-1569.
22. Scherr, M., Rossi, J. J., Sczakiel, G., and Patzel, V. (2000). RNA accessibility prediction: a theoretical approach is consistent with experimental studies in cell extracts. Nucleic Acids Res. 28: 2455-2461.
23. Ryan, K. M., and Birnie, G. D. (1996). Myc oncogenes: the enigmatic family. Biochem. J. 314: 713-721.
24. Sollazzo, M. R., et al. (1999). Increased c-myc oncogene expression in Ewing's sarcoma: correlation with Ki67 proliferation index. Tumori 85: 167-173.
25. Ioannidis, P., et al. (2001). C-MYC and IGF-II mRNA-binding protein (CRD-BP/IMP-1) in benign and malignant mesenchymal tumors. Int. J. Cancer 94: 480-484.
26. Arvand, A., and Denny, C. T. (2001). Biology of EWS/ETS fusion in Ewing's family tumors. Oncogene 29: 5747-5754.
27. Bertolotti, A., Melot, T., Acker, J., Vigneron, M., Delattre, O., and Tora, L. (1998). EWS, but not EWS-Fli-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes. Mol. Cell. Biol. 18: 1489-1497.
28. Dhulipara, P. D., Lee, L., Rao, V. N., and Reddy, E. S. (1998). Fli-1b is generated by usage of differential splicing and alternative promoter. Oncogene 17: 1149-1157.
29. Bacci, G., et al. (2002). Neoadjuvant chemotherapy for Ewing's tumor of bone: recent experience at the Rizzoli Orthopaedic Institute. Eur. J. Cancer 38: 2243-2251.
30. Burdash, S., and Jurgens, H. (2002). High-dose chemoradiotherapy (HDC) in the Ewing family of tumors (EFT). Crit. Rev. Oncol. Hematol. 41: 169-189.
31. Paulussen, M., et al. (1998). Ewing's tumors with primary lung metastases: survival analysis of 114 (European Intergroup) Cooperative Ewing's sarcoma studies patients. Oncology 16: 3044-3052.
32. Athale, U. H., et al. (2001). Use of reverse transcriptase polymerase chain reaction for diagnosis and staging of alveolar rhabdomyosarcoma, Ewing sarcoma family of tumors, and desmoplastic small round cell tumor. J. Pediatr. Hematol. Oncol. 23: 9-104.