Viability of poliovirus/rhinovirus VPg chimeric viruses and identification of an amino acid residue in the VPg gene critical for viral RNA replication

Journal of Virology

Volumn 77, Issue 13, 2003, Pages 7434-7443

  Cheney, I Wayne ^a   Naim, Suhaila ^a   Shim, Jae Hoon ^a   Reinhardt, Meghan ^a   Pai, Bharati ^a   Wu, Jim Z ^a   Hong, Zhi ^a   Zhong, Weidong ^a  

^a Ribapharm Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN VPG; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CHIMERA; CONTROLLED STUDY; GENE MUTATION; GENETIC TRANSFECTION; GENETIC VARIABILITY; HELA CELL; HUMAN; HUMAN CELL; PICORNAVIRUS; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; RHINOVIRUS; RNA REPLICATION; RNA SYNTHESIS; VIRUS PARTICLE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; CHIMERA; DNA PRIMERS; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; POLIOVIRUS; RHINOVIRUS; RNA, VIRAL; SEQUENCE HOMOLOGY, AMINO ACID; VIRAL CORE PROTEINS;

EID: 0038468333     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.13.7434-7443.2003     Document Type: Article

References (43)

1. Andries, K., B. Dewindt, J. Snoeks, L. Wouters, H. Moereels, P. J. Lewi, and P. A. J. Janssen. 1990. Two groups of rhinoviruses revealed by a panel of antiviral compounds present sequence divergence and differential pathogenicity. J. Virol. 64:1117-1123.
2. Arnold, E., J. W. Erickson, G. Shay Fout, E. A. Frankenberger, H.-J. Hecht, M. Luo, M. G. Rossmann, and R. R. Rueckert. 1984. Virion orientation in cubic crystals of human common cold virus HRV14. J. Mol. Biol. 177:417-430.
3. Arnold, J. J., and C. E. Cameron. 2000. Poliovirus RNA-dependent RNA polymerase (3Dpol). Assembly of stable elongation-competent complexes by using a symmetrical primer-template substrate (sym/sub). J. Biol. Chem. 275:5329-5336.
4. Baker, R. T., S. A. Smith, R. Marano, J. McKee, and P. G. Board. 1994. Protein expression using cotranslational fusion and cleavage of ubiquitin. J. Biol. Chem. 269:25381-25386.
5. Barton, D. J., E. P. Black, and J. B. Flanegan. 1995. Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA. J. Virol. 69:5516-5527.
6. Butterworth, B. E., R. R. Grunert, B. D. Korant, K. Longberg-Holm, and F. H. Yin. 1976. Replication of rhinoviruses. Arch. Virol. 51:169-189.
7. Callahan, P. L., S. Mizutani, and R. J. Colonno. 1985. Molecular cloning and complete sequence determination of the RNA genome of human rhinovirus type 14. Proc. Natl. Acad. Sci. USA 82:732-736.
8. Cao, X., R. J. Kuhn, and E. Wimmer. 1993. Replication of poliovirus RNA containing two VPg coding sequences leads to a specific deletion event. J. Virol. 67:5572-5578.
9. Crotty, S., D. Maag, J. J. Arnold, W. Zhong, J. Y. N. Lau, Z. Hong, R. Andino, and C. E. Cameron. 2000. The broad-spectrum antiviral ribonucleoside ribavirin is an RNA virus mutagen. Nat. Med. 6:1375-1379.
10. Erickson, J. W., E. A. Frankenberger, M. G. Rossmann, G. Shay Fout, K. C. Medappa, and R. R. Rueckert. 1982. Crystallization of a common cold virus, human rhinovirus 14: "isomorphism" with poliovirus crystals. Proc. Natl. Acad. Sci. USA 80:931-934.
11. Flanegan, J. B., and D. Baltimore. 1977. A poliovirus-specific primer-dependent RNA polymerase able to copy poly(A). Proc. Natl. Acad. Sci. USA 74:3677-3680.
12. pol. J. Virol. 75:10969-10978.
13. pol) by ubiquitin-protease-mediated cleavage in Escherichia coli. Protein Expr. Purif. 17:128-138.
14. pol). J. Biol. Chem. 275:25523-25532.
15. Hansen, J. L., A. M. Long, and S. C. Schultz. 1997. Structure of the RNA dependent RNA polymerase of poliovirus. Structure 5:1109-1122.
16. Hope, D. A., S. E. Diamond, and K. Kirkegaard. 1997. Genetic dissection of interaction between poliovirus 3D polymerase and viral protein 3AB. J. Virol. 71:9490-9498.
17. Horton, R. M., Z. L. Cai, S. N. Ho, and L. R. Pease. 1990. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. BioTechniques 8:528-535.
18. Hung, M., C. S. Gibbs, and M. Tsiang. 2002. Biochemical characterization of rhinovirus RNA-dependent RNA polymerase. Antiviral Res. 56:99-114.
19. Kuhn, R. J., H. Tada, M. F. Y. Wong, J. J. Dunn, B. L. Semler, and E. Wimmer. 1988. Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants. Proc. Natl. Acad. Sci. USA 85:519-523.
20. pol. J. Biol. Chem. 269:66-70.
21. Lee, W.-M., W. Wang, and R. R. Rueckert. 1994. Complete sequence of the RNA genome of human rhinovirus 16, a clinically useful common cold virus belonging to the ICAM-1 receptor group. Virus Genes 9:177-181.
22. Lee, Y. F., A. Nomoto, B. M. Detjen, and E. Wimmer. 1977. A protein covalently linked to poliovirus genome RNA. Proc. Natl. Acad. Sci. USA 74:59-63.
23. Lyle, J. M., A. Clewell, K. Richmond, O. C. Richards, D. A. Hope, S. C. Schultz, and K. Kirkegaard. 2002. Similar structural basis for membrane localization and protein priming by an RNA-dependent RNA polymerase. J. Biol. Chem. 277:16324-16331.
24. McKnight, K. L., and S. M. Lemon. 1996. Capsid coding sequence is required for efficient replication of human rhinovirus 14 RNA. J. Virol. 70:1941-1952.
25. McKnight, K. L., and S. M. Lemon. 1998. The rhinovirus type 14 genome contains an internally located RNA structure that is required for viral replication. RNA 4:1569-1584.
26. Molla, A., A. V. Paul, and E. Wimmer. 1991. Cell-free de novo synthesis of poliovirus. Science 254:1647-1651.
27. Pathak, H. B., S. K. B. Ghosh, A. W. Roberts, S. D. Sharma, J. D. Yoder, J. J. Arnold, D. W. Gohara, D. J. Barton, A. V. Paul, and C. E. Cameron. 2002. Structure-function relationships of the RNA-dependent RNA polymerase from poliovirus (3Dpol). J. Biol. Chem. 277:31551-31562.
28. Paul, A. V., J. H. van Boom, D. Filippov, and E. Wimmer. 1998. Protein-primed RNA synthesis by purified poliovirus RNA polymerase. Nature (London) 393:280-284.
29. Paul, A. V., E. Reider, D. W. Kim, J. H. van Boom, and E. Wimmer. 2000. Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg. J. Virol. 74:10359-10370.
30. Paul, A. V., J. Peters, J. Mugavero, J. Yin, J. H. van Boom, and E. Wimmer. 2003. Biochemical and genetic studies of the VPg uridylylation reaction catalyzed by the RNA polymerase of poliovirus. J. Virol. 77:891-904.
31. Plotch, S. J., O. Palant, and Y. Gluzman. 1989. Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli. J. Virol. 63:216-225.
32. Plotch, S. J., and O. Palant. 1995. Poliovirus protein 3AB forms a complex with and stimulates the activity of the viral RNA polymerase, 3Dpol. J. Virol. 69:7169-7179.
33. Reider, E., A. V. Paul, D. W. Kim, J. H. van Boom, and E. Wimmer. 2000. Genetic and biochemical studies of poliovirus cis-acting replication element cre in relation to VPg uridylylation. J. Virol. 74:10371-10380.
34. Reuer, Q., R. J. Kuhn, and E. Wimmer. 1990. Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg. J. Virol. 64:2967-2975.
35. Rohll, J. B., D. H. Moon, D. J. Evans, and J. W. Almond. 1995. The 3′ untranslated region of picornavirus RNA: features required for efficient genome replication. J. Virol. 69:7835-7844.
36. Rossmann, M. G., E. Arnold, J. W. Erickson, E. A. Frankenberger, J. P. Griffith, H.-J. Hecht, J. E. Johnson, G. Kamer, M. Luo, A. G. Mosser, R. R. Rueckert, B. Sherry, and G. Vriend. 1985. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature 317:145-153.
37. Sherry, B., and R. Rueckert. 1985. Evidence for at least two dominant neutralization antigens on human rhinovirus 14. J. Virol. 53:137-143.
38. Stanway, G., P. J. Hughes, R. C. Mountford, P. D. Minor, and J. W. Almond. 1984. The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Res. 12:7859-7875.
39. Todd, S., J. S. Towner, and B. L. Semler. 1997. Translation and replication properties of the human rhinovirus genome in vivo and in vitro. Virology 229:90-97.
40. Turner, R. B. 2001. The treatment of rhinovirus infections: progress and potential. Antiviral Res. 49:1-14.
41. Wang, W., and B. A. Malcolm. 1999. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange site-directed mutagenesis. BioTechniques 26:680-682.
42. Wimmer, E. 1982. Genome-linked proteins of viruses. Cell 28:199-201.
43. pol with VPg and with genetic variants of 3AB. J. Virol. 72:6732-6741.