Cloning of a Heliothis virescens 110 kDa aminopeptidase N and expression in Drosophila S2 cells

Insect Biochemistry and Molecular Biology

Volumn 33, Issue 5, 2003, Pages 499-508

  Banks, D J ^a,c   Hua, G ^b   Adang, M J ^a,b  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Aminopeptidase; Aminopeptidase N; Bacillus thuringiensis; Binding protein; CrylAc; CrylFa; Drosophila S2 cells; Heliothis virescens; Toxin

Indexed keywords

BACILLUS THURINGIENSIS; DROSOPHILA; HELIOTHIS; HELIOTHIS VIRESCENS; VECTORS;

EID: 0038402735     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(03)00022-5     Document Type: Article

References (37)

1. Azzouz, N., Kedees, M.H., Gerold, P., Becker, S., Dubremetz, J., Klenk, H., et al. 2000. An early step of glycosylphosphatidyl-inositol anchor biosynthesis is abolished in lepidopteran insect cells following baculovims infection. Glycobiology 10 (2), 177-183.
2. Banks, D.J., Jurat-Fuentes, J.L., Dean, D.H., Adang, M.J., 2001. Bacillus thuringiensis CrylAc and CrylFa δ-endotoxin binding to a novel 110 kDa aminopeptidase in Heliothis virescens in not N-acetylgalactosamine mediated. Insect Biochem. Mol. Biol. 31, 909-918.
3. Denolf, P., Hendrickx, K., Van Damme, J., Jansens, S., Peferoen, M., Degheele, D., Van Rie, J., 1997. Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N enzymes related to Bacillus thuringiensis toxin-binding proteins. Eur. J. Biochem. 15, 748-761.
4. Emmerling, M., Chandler, D., Sandeman, M., 2001. Molecular cloning of three cDNAs encoding aminopeptidases from the midgut of Helicoverpa punctigera, the Australian native budworm. Insect Biochem. Mol. Biol. 31, 899-907.
5. Frohman, M.A., Dush, M.K., Martin, G.R., 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. 85, 8998-9002.
6. Garczynski, S.F., Crim, J.W., Adang, M.J., 1991. Identification of putative insect brush border membrane-binding molecules specific to Bacillus thuringiensis δ-endotoxin by protein blot analysis. Appl. Environ. Microbiol. 57, 2816-2820.
7. Gill, M., Ellar, D., 2002. Transgenic Drosophila reveals a functional in vivo receptor for the Bacillus thuringiensis toxin CrylAcl. Insect Mol. Biol. 11, 619-625.
8. Gill, S.S., Cowles, E.A., Francis, V., 1995. Identification, isolation, and cloning of a Bacillus thuringiensis CrylAc toxin-binding protein from the midgut of the lepidopteran insect Heliothis virescens. J. Biol. Chem. 270, 27277-27282.
9. Hua, G., Tsukamoto, K., Rasilo, M.-L., Ikezawa, H., 1998a. Molecular cloning of a GPI-anchored aminopeptidase N from Bombyx mori midgut: a putative receptor for Bacillus thuringiensis CrylA toxin. Gene 214, 177-185.
10. Hua, G., Tsukamoto, K., Taguchi, R., Tomita, M., Miyajima, S., Ikezawa, H., 1998b. Characterization of aminopeptidase N from the brush border membrane of the larvae midgut of silkworm, Bombyx mori as a zinc enzyme. Biochim. Biophys. Acta 1383, 301-310.
11. Ihara, H., Kuroda, E., Wadano, A., Himeno, M., 1993. Specific toxicity of δ-endotoxins from Bacillus thuringiensis to Bombyx mori. Biosci. Biotech. Biochem. 57, 200-204.
12. Jarvis, D.L., Finn, E.E., 1995. Biochemical analysis of the N-glycosylation pathway in baculovirus-infected lepidopteran insect cells. Virology, 212(2), 500-511.
13. Jarvis, D.L., Fleming, J.-A., Kovacs, G.R., Summers, M.D., Gaurino, L.A., 1990. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Biotechnology 8, 950-955.
14. Keeton, T.P., Bulla, L.A., 1997. Ligand specificity and affinity of BT-R1, the Bacillus thuringiensis CrylA toxin receptor from Manduca Sexta, expressed in mammalian and insect cell cultures. Appl. Environ. Microbiol. 63, 3419-3425.
15. Knight, P.J.K., Crickmore, N., Ellar, D.J., 1994. The receptor for Bacillus thuringiensis CrylAc delta-endotoxin in the brush border of the lepidopteran Manduca sexta is aminopeptidase. J. Mol. Microbiol. 11, 429-436.
16. Lee, M.K., You, T.H., Gould, F.L., Dean, D.H., 1999. Identification of residues in domain III of Bacillus thuringiensis CrylAc toxin that affect binding and toxicity. Appl. Environ. Microbiol. 65, 4513-4520.
17. Luo, K., Sangadala, S., Masson, L., Mazza, A., Brousseau, R., Adang, M.J., 1997. The Heliothis virescens 170 kDa aminopeptidase functions as 'receptor A' by mediating specific Bacillus thuringiensis δ-endotoxin binding and pore formation. Insect Biochem. Mol. Biol. 27, 735-743.
18. Luo, K., McLachlin, J.R., Brown, M.R., Adang, M.J., 1999. Expression of a glycosylphosphatidylinositol-linked Manduca sexta aminopeptidase N in insect cells. Protein Expres. Purif. 17, 113-122.
19. Masson, L., Lu, Y.-J., Mazza, A., Brousseau, R., Adang, M.J., 1995. The CrylAc receptor purified from Manduca sexta displays multiple specificities. J. Biol. Chem. 270, 20309-20315.
20. 1, a cadherin-related receptor for Manduca sexta for Bacillus thuringiensis CrylAb toxin. Protein Expres. Purif. 22, 141-147.
21. Nagamatsu, Y., Toda, S., Koike, T., Miyoshi, Y., Shigematsu, S., Kogure, M., 1998. Cloning, sequencing, and expression of the Bombyx mori receptor for Bacillus thuringiensis insecticidal CrylAa toxin. Biosci. Biotechnol. Biochem. 62, 727-734.
22. Oltean, D.I., Pullikuth, A.K., Lee, H.-K., Gill, S.S., 1999. Partial purification and characterization of Bacillus thuringiensis CrylA toxin receptor A from Heliothis virescens and cloning of the corresponding cDNA. Appl. Environ. Microbiol. 65, 4760-4766.
23. Peferoen, M., 1997. Progress and prospects for field use of Bt genes in crops. Trends Biotechnol. 15, 173-177.
24. 2+ release from intracellular stores in Cf1 cells (Choristoneura fumiferana, Lepidoptera). J. Exp. Biol. 201, 1851-1858.
25. Rajagopal, R., Sivakumar, S., Agrawal, N., Malhotra, P., Bhatnagar, R.K., 2002. Silencing of midgut aminopeptidase N of Spodoptera litura by dsRNA establishes its role as Bt toxin receptor. J. Biol. Chem. 277, 46849-46851.
26. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
27. + efflux in vitro. J. Biol. Chem. 269, 10088-10092.
28. Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., et al. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62 (3), 775-806.
29. Schwartz, J.L., Garneau, L., Savaria, D., Masson, L., Brousseau, R., Rousseau, E., 1993. Lepidopteran specific crystal toxins from Bacillus thuringiensis form cation and anion selective channels in planar lipid bilayers. J. Membr. Biol. 132, 53-62.
30. Schwartz, J.L., Yiang-Jiang, L., Sohnlein, P., Brousseau, R., Laprade, R., Masson, L., et al. 1997. Ion channels formed in planar lipid bilayers by Bacillus thuringiensis toxins in the presence of Manduca sexta midgut receptors. FEBS Lett. 412, 270-276.
31. Simpson, R.M., Newcomb, R.D., 2000. Binding of Bacillus thuringiensis δ-endotoxins CrylAc and CrylBa to a 120 kDa aminopeptidase N of Epiphyas postvittana purified from both brush border membrane vesicles and baculovirus infected Sf9 cells. Insect Biochem. Mol. Biol. 30, 1069-1078.
32. Terra, W.R., Ferreira, C., 1994. Insect digestive enzymes: properties, compartmentalization and function. Comp. Biochem. Physiol. B 109, 1-62.
33. Vadlamudi, R.K., Weber, E., Inhae, J., Tae, H.J., Bulla, L.A., 1995. Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis. J. Biol. Chem. 270, 5490-5494.
34. Van Die, I., Van Tetering, A., Bakker, H., Van den Eijnden, D.H., Joziasse, D.H., 1996. Glycosylation in lepidopteran insect cells: identification of a β1 4-N-acetylgalactosaminyltransferase involved in the synthesis of complex-type oligosaccharide chains, Glycobiology 6, 157-164.
35. Varma, R., Mayor, S., 1998. GPI-anchored proteins are organized in submicron domains at the cell surface. Nature 20, 798-801.
36. Wolfersberger, M.G., Luthy, P., Maurer, A., Parenti, P., Sacchi, V.F., Giordana, B., et al. 1987. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae). Comp. Biochem. Physiol. 86, 301-308.
37. Zhuang, M., Oltean, D.I., Gomez, I., Pullikuth, A.K., Soberon, M., Bravo, A., et al. 2002. Heliothis virescens and Manduca sexta lipid rafts are involved in CrylA toxin binding to the midgut epithelium and subsequent pore formation. J. Biol. Chem. 277, 13863-13872.