Bacillus thuringiensis Cry1Ac and Cry1Fa δ-endotoxin binding to a novel 110 kDa aminopeptidase in Heliothis virescens is not N-acetylgalactosamine mediated

Insect Biochemistry and Molecular Biology

Volumn 31, Issue 9, 2001, Pages 909-918

  Banks, David J ^a   Jurat Fuentes, Juan L ^b   Dean, Donald H ^c   Adang, Michael J ^a,b  

^a University of Georgia ^*  (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c OHIO STATE UNIVERSITY   (United States)

Author keywords

Endotoxin; Aminopeptidase; Bacillus thuringiensis; Binding protein; Cry1; Heliothis virescens; N acetylgalactosamine

Indexed keywords

AMINOPEPTIDASE; AMINOPEPTIDASE N, INSECT; BACILLUS THURINGIENSIS CRYSTAL PROTEIN; BACTERIAL PROTEIN; BIOTIN; CRY1AC ENDOTOXIN; CRY1FA SIGMA ENDOTOXIN; DODECYL SULFATE SODIUM; ENDOTOXIN; INSECT PROTEIN; IODINE 125; LECTIN; LIGAND; N ACETYLGALACTOSAMINE; PERIODATE; PERIODIC ACID; RADIOACTIVE IODINE; SOYBEAN AGGLUTININ; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BACILLUS THURINGIENSIS; BRUSH BORDER; HELIOTHIS VIRESCENS; ISOTOPE LABELING; LEPIDOPTERA; MEMBRANE VESICLE; MOLECULAR GENETICS; MOTH; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; SEQUENCE HOMOLOGY; STAINING; WESTERN BLOTTING;

ACETYLGALACTOSAMINE; AMINO ACID SEQUENCE; AMINOPEPTIDASES; ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BIOTIN; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; HEMOLYSIN PROTEINS; INSECT PROTEINS; IODINE RADIOISOTOPES; LECTINS; LIGANDS; MOLECULAR SEQUENCE DATA; MOTHS; PERIODIC ACID; PLANT LECTINS; POLYMERASE CHAIN REACTION; SODIUM DODECYL SULFATE; SOYBEAN PROTEINS; STAINING AND LABELING;

BACILLUS THURINGIENSIS; GLYCINE MAX; HELICOVERPA PUNCTIGERA; HELIOTHIS PUNCTIGERA; HELIOTHIS VIRESCENS;

EID: 0035954588     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(01)00038-8     Document Type: Article

References (35)

1. Integrative model for binding of Bacillus thuringiensis toxins in susceptible and resistant larvae of the diamond back moth (Plutella xylostella)
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
3. N-acetylgalactosamine on the putative insect receptor aminopeptidase N is recognized by a site on the domain III lectin-like fold of a Bacillus thuringiensis insecticidal toxin
4. The Bacillus thuringiensis Cry1Ac toxin-induced permeability change in Manduca sexta midgut brush border membrane vesicles proceeds by more than one mechanism
5. Isolation and characterization of a novel insecticidal crystal protein gene from Bacillus thuringiensis subsp. aizawai
6. A new aminopeptidase from diamondback moth provides evidence for a gene duplication event in Lepidoptera
7. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis
8. Biotinylation of Bacillus thuringiensis insecticidal crystal proteins
9. Identification of putative insect brush border membrane-binding molecules specific to Bacillus thuringiensis δ-endotoxin by protein blot analysis
10. Identification, isolation, and cloning of a Bacillus thuringiensis Cry1Ac toxin-binding protein from the midgut of the lepidopteran insect Heliothis virescens
11. Selection and genetic analysis of a Heliothis virescens (Lepidoptera: Noctuidae) strain with high levels of resistance to Bacillus thuringiensis toxins
12. Bacillus thuringiensis crystal protein Cry1Ab and Cry1Fa share a high affinity binding site in Plutella xylostella (L.)
13. Binding of Bacillus thuringiensis Cry1Ac toxin to Manduca sexta aminopeptidase-N receptor is not directly related to toxicity
14. Importance of Cry1 δ-endotoxin domain II loops for binding specificity in Heliothis virescens (L.)
15. The receptor for Bacillus thuringiensis Cry1Ac delta-endotoxin in the brush border of the lepidopteran Manduca sexta is aminopeptidase
16. The crystal δ-endotoxin of Bacillus thuringiensis: Models for their mechanisms of action on the insect gut
17. Aminopeptidase N purified from gypsy moth brush border membrane vesicles is a specific receptor for Bacillus thuringiensis Cry1Ac toxin
18. Identification of residues in domain III of Bacillus thuringiensis Cry1Ac toxin that affect binding and toxicity
19. A 106 kDa form of aminopepidase is a receptor for Bacillus thuringiensis Cry1C δ-endotoxin in the brush border membrane of Manduca sexta
20. The Heliothis virescens 170 kDa aminopeptidase functions as "receptor A" by mediating specific Bacillus thuringiensis δ-endotoxin binding and pore formation
21. Toxicity, binding and permeability analyses of four Bacillus thuringiensis Cry1 δ-endotoxins using brush border membrane vesicles of Spodoptera exigua and Spodoptera frugiperda
22. Expression of a glycosylphosphatidylinositol-linked Manduca sexta Aminopeptidase N in insect cells
23. Partial purification and characterization of Bacillus thuringiensis CrylA toxin receptor A from Heliothis virescens and cloning of the corresponding cDNA
24. Progress and prospects for field use of Bt genes in crops
25. Role of domain II, loop 2 residues of Bacillus thuringiensis Cry1Ab δ-endotoxin in reversible and irreversible binding to Manduca sexta and Heliothis virescens
26. Protein engineering of Bacillus thuringiensis δ-endotoxin: Mutations at domain II of Cry1Ab enhance receptor affinity and toxicity toward gypsy moth larvae
27. A mixture of Manduca sexta aminopeptidase and phosphatase enhances Bacillus thuringiensis insecticidal Cry1Ac toxin binding and 86Rb+-K+ efflux in vitro
28. Cross-resistance of the diamondback moth indicates altered interaction with domain II of Bacillus thuringiensis toxins
29. Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis
30. Specificity of Bacillus thuringiensis δ-endotoxins: Importance of specific receptors on the brush border membrane of the midgut of target insects
31. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae)
32. Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation
33. Aminopeptidase N from Bombyx mori as a candidate for the receptor of Bacillus thuringiensis Cry1Aa toxin