Expression of a glycosylphosphatidylinositol-linked Manduca sexta aminopeptidase N in insect cells

Protein Expression and Purification

Volumn 17, Issue 1, 1999, Pages 113-122

  Luo, Ke ^a   McLachlin, Jeanne R ^a   Brown, Mark R ^a   Adang, Michael J ^a,b  

^a UNIVERSITY OF GEORGIA   (United States)

^b University of Georgia   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIBODY SPECIFICITY; CELL MEMBRANE; COMPLEMENTARY DNA; ENZYME ANALYSIS; ENZYME SYNTHESIS; GLYCOSYLATION; GLYCOSYLPHOSPHATIDYLINOSITOL; INSECT CELL CULTURE; LEPIDOPTERA; MICROSOMAL AMINOPEPTIDASE; MOLECULAR CLONING; MOLECULAR SIZE; PHOSPHOLIPASE; PLASMID VECTOR; TOBACCO HORN WORM; TRANSIENT TRANSFECTION; WESTERN BLOTTING;

HEXAPODA; INSECTA; LEPIDOPTERA; MANDUCA SEXTA; NICOTIANA TABACUM; SPODOPTERA FRUGIPERDA; VERTEBRATA;

EID: 0033213235     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1122     Document Type: Article

References (44)

1. Kenny A. J., Stephenson S. L., Turner A. J. Cell surface peptidases. Kenny A. J., Turner A. J. Mammalian Ectoenzymes. 1987;169-210 Elsevier, Amsterdam.
2. Takasaki S., Erickson R. H., Kim Y. S., Kochibe N., Kobata A. N-linked neutral sugar chains of aminopeptidase N purified from rat small intestinal brush-border membrane. Biochemistry. 30:1991;9102-9110.
3. Tomita M., Obara H., Takesue Y., Tamura H.-O., Miyajima S., Taguchi R., Ikezawa H. Purification of glycosylphosphatidylinositol-anchoring aminopeptidase N from the plasma membrane of larval midgut epithelial cells of the silkworm, Bombyx mori. Int. J. Biochem. 26:1994;977-986.
4. Garczynski S. F., Adang M. J. Bacillus thuringiensis CryIA(c) δ-endotoxin binding aminopeptidase in the Manduca sexta midgut has a glycosyl-phosphatidylinositol anchor. Insect Biochem. Mol. Biol. 25:1995;409-415.
5. Luo K., Lu Y. J., Adang M. J. A 106 kDa form of aminopeptidase is a receptor for Bacillus thuringiensis CryIC δ-endotoxin in the brush border membrane of Manduca sexta. Insect Biochem. Mol. Biol. 26:1996;783-791.
6. Luo K., Sangadala S., Masson L., Mazza A., Brousseau R., Adang M. J. The Heliothis virescens 170-kDa aminopeptidase functions as 'Receptor A' by mediating specific Bacillus thuringiensis Cry1A δ-endotoxin binding and pore formation. Insect Biochem. Mol. Biol. 27:1997;735-743.
7. Luo K., Tabashnik B. E., Adang M. J. Binding of Bacillus thuringiensis Cry1Ac toxin to aminopeptidase in susceptible and resistant diamondback moths (Plutella xylostella). Appl. Environ. Microbiol. 63:1997;1024-1027.
8. McConville M. J., Ferguson M. A. J. The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes. Biochem. J. 294:1993;305-324.
9. Fujii H., Nakajima M., Saiki I., Yoneda J., Azuma I., Tsuruo T. Human-melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13. Clin. Exp. Metastasis. 13:1995;337-344.
10. +-dependent amino acid transport in bovine renal brush-border membranes. Biochem. J. 290:1993;59-65.
11. Delmas B., Gelfi J., Haridon R. L., Vogel L. K., Sjostrom H., Noren O., Laude H. Aminopeptidase N is a major receptor for the enteropathogenic coronavirus TGEV. Nature. 357:1992;417-419.
12. Yeager C. L., Ashmun R. A., Williams R. K., Cardellichio C. B., Shapiro L. H., Look A. T., Holmes K. V. Human aminopeptidase N is a receptor for human coronavirus 229E. Nature. 357:1992;420-422.
13. Tresnan N. B., Levis R., Holmes K. V. Feline aminopeptidase N serve as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70:1996;8669-8674.
14. Benbacer L., Kut E., Besnardeau L., Laude H., Delmas B. Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus. J. Virol. 71:1997;734-737.
15. McLaughlin S., Aderem A. The myristoyl-electrostatic switch: A modulator of reversible protein-membrane interactions. Trends Biochem. Sci. 20:1995;272-276.
16. Kolb A. F., Maile J., Heister A., Siddell S. G. Characterization of functional domains in the human coronavirus HCV 229E receptor. J. Gen. Virol. 77:1996;2515-2521.
17. Knight P. J. K., Crickmore N., Ellar D. J. The receptor for Bacillus thuringiensis CryIA(c) delta-endotoxin in the brush border membrane is aminopeptidase N. Mol. Microbiol. 11:1994;429-436.
18. + leakage in vitro. J. Biol. Chem. 269:1994;10088-10092.
19. Gill S., Cowles E. A., Francis V. Identification, isolation, and cloning of a Bacillus thuringiensis CryIAc toxin-binding protein from the midgut of the lepidopteran insect Heliothis virescens. J. Biol. Chem. 270:1995;27277-27282.
20. Valaitis A., Lee M. K., Rajamohan F., Dean D. H. Brush border membrane aminopeptidase-N in the midgut of the gypsy moth serves as the receptor for the CryIA(c) δ-endotoxin of Bacillus thuringiensis. Insect Biochem. Mol. Biol. 25:1995;1143-1151.
21. Yaoi K., Kadotani T., Kuwana H., Shinkawa A., Takahashi T., Iwahana H., Sato M. Aminopeptidase N from Bombyx mori as a candidate for the receptor of Bacillus thuringiensis Cry1Aa toxin. Eur. J. Biochem. 246:1997;652-657.
22. Masson L., Lu Y., Mazza A., Brosseau R., Adang M. J. The CryIA(c) receptor purified from Manduca sexta displays multiple specificities. J. Biol. Chem. 270:1995;20309-20315.
23. Schwartz J.-L., Lu Y. J., Soehnlein P., Brousseau R., Masson L., Laprade R., Adang M. J. Ion channels formed in planar lipid bilayers by Bacillus thuringiensis toxins in the presence of Manduca sexta midgut receptors. FEBS Lett. 412:1997;270-276.
24. Knight P. J. K., Knowles B. H., Ellar D. J. Molecular cloning of an insect aminopeptidase N that serves as a receptor for Bacillus thuringiensis CryIA(c) toxin. J. Biol. Chem. 270:1995;17765-17770.
25. Denolf P., Hendrickx K., Van Damme J., Jansens S., Peferoen M., Degheele D., Van Rie J. Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N enzymes related to Bacillus thuringiensis toxin-binding proteins. Eur. J. Biochem. 248:1997;748-761.
26. Hua G., Tsukamoto K., Rasilo M., Ikezawa H. Molecular cloning of a GPI-anchored aminopeptidase N from Bombyx mori midgut: A putative receptor for Bacillus thuringiensis CryIA toxin. Gene. 214:1998;177-185.
27. Davies A., Morgan B. P. Expression of the glycosylphosphatidylinositol-linked complement-inhibiting protein CD59 antigen in insect cells using a baculovirus vector. Biochem. J. 295:1993;889-896.
28. Richardson M. A., Smith D. R. J., Kemp D. H., Tellam R. L. Native and baculovirus-expressed forms of the immunoprotective protein BM86 from Boophilus microplus are anchored to the cell membrane by a glycosylphosphatidylinositol linkage. Insect Mol. Biol. 1:1993;139-147.
29. Chaudhri M., Steverding D., Kittelberger D., Tjia S., Overath P. Expression of glycosylphosphatidylinositol-anchored Trypanosoma brucei transferrin-binding protein complex in insect cells. Proc. Natl. Acad. Sci. USA. 91:1994;6443-6447.
30. Kennard M. L., Shimizu K. Y., Gabathuler R., Rothenberger S., Therlmann D., Jefferies W. A. Expression of cell surface GPI-anchored human p97 in baculovirus-infected insect cells. Biotech. Bioeng. 55:1997;41-53.
31. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
32. Morris T. D., Miller L. K. Promoter influence on baculovirus-mediated gene expression in permissive and nonpermissive insect cell lines. J. Virol. 66:1992;7397-7405.
33. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Dossec R. D. The complete DNA sequence of Autograph californica nuclear polyhedrosis virus. Virology. 202:1994;586-605.
34. Vaughn J. L., Goodwin R. H., Tompkins G. L., McCawley P. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera:Noctuidae). In Vitro Cell. Dev. Biol. 13:1977;213-217.
35. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
36. Wolfersberger M. G., Luthy P., Maurer A., Parenti P., Sacchi V. F., Giordana B., Hanozet G. M. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae). Comp. Biochem. Physiol. 86A:1987;301-308.
37. Ferre J., Real M. D., Van Rie J., Jansens S., Peferoen M. Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor. Proc. Natl. Acad. Sci. USA. 88:1991;5119-5123.
38. Lu Y., Adang M. J. Conversion of Bacillus thuringiensis CryIAc-binding aminopeptidase to a soluble form by endogenous phosphatidylinositol phospholipase C. Insect Biochem. Mol. Biol. 226:1996;33-40.
39. Kozak M. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:1987;8125-8132.
40. van Die A., van Tetering A., Bakker H., van den Eijnden D. H., Jozaisse D. H. Glycosylation in lepidopteran insect cells: Identification of a B-1,4-N-acetylgalactosaminyltransferase involved in the synthesis of complex-type oligosaccharides. Glycobiology. 6:1996;157-164.
41. Singh N., Singleton D., Tartakoff A. M. Anchoring and degradation of glycolipid-anchored proteins by L929 versus by LM-TK-mouse fibroblasts: Implications for anchor biosynthesis. Mol. Cell Biol. 11:1991;2362-2374.
42. Delahunty M. D., Stafford F. J., Yuan L. C., Shaz D., Bonifacino J. S. Uncleaved signals for glycosylphosphatidylinositol anchoring cause retention of precursor proteins in the endoplasmic reticulum. J. Biol. Chem. 16:1993;12017-12027.
43. Look A. T., Ashmun R. A., Shapiro L. H., Peiper S. C. Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N. J. Clin. Invest. 83:1989;1299-1307.
44. Garczynski S. F., Crim J. W., Adang M. J. Identification of putative brush border membrane binding proteins specific to Bacillus thuringiensis δ-endotoxin by protein blot analysis. Appl. Environ. Microbiol. 57:1991;2816-2820.