Structural characterization of respiratory syncytial virus fusion inhibitor escape mutants: Homology model of the F protein and a syncytium formation assay

Virology

Volumn 311, Issue 2, 2003, Pages 275-288

  Morton, Craig J ^a,c   Cameron, Rachel ^a   Lawrence, Lynne J ^b   Lin, Bo ^a   Lowe, Melinda ^b   Luttick, Angela ^a   Mason, Anthony ^a   McKimm Breschkin, Jenny ^b   Parker, Michael W ^c   Ryan, Jane ^a   Smout, Michael ^d   Sullivan, Jayne ^a   Tucker, Simon P ^a   Young, Paul R ^d  

^a Biota Holdings Limited   (Australia)

^b CSIRO   (Australia)

^c ST VINCENT'S INSTITUTE OF MEDICAL RESEARCH   (Australia)

^d UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Antiviral; Assay; Fusion protein; Homology model; Inhibitor; Respiratory syncytial virus

Indexed keywords

VIRUS FUSION PROTEIN;

ARTICLE; ASSAY; BINDING SITE; CRYSTAL STRUCTURE; ELECTRON MICROSCOPY; IN VITRO STUDY; LOWER RESPIRATORY TRACT INFECTION; MEMBRANE FUSION; MOLECULAR MODEL; MUTANT; NEWCASTLE DISEASE PARAMYXOVIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; SYNCYTIUM;

NEWCASTLE DISEASE VIRUS; RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS; SYNCYTIAL VIRUS;

EID: 0038045777     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0042-6822(03)00115-6     Document Type: Article

References (38)

1. Bourgeois C., Corvaisier C., Bour J.B., Kohli E., Pothier P. Use of synthetic peptides to locate neutralizing antigenic domains on the fusion protein of respiratory syncytial virus. J. Gen. Virol. 72:1991;1051-1058.
2. Calder L.J., Gonzalez-Reyes L., Gracia-Barreno B., Wharton S.A., Skehel J.J., Wiley D.C., Melero J.A. Electron microscopy of the human respiratory syncytial virus fusion protein and complexes that it forms with monclonal antibodies. Virology. 271:2000;122-131.
3. Cane P.A. Molecular epidemiology of respiratory syncytial virus. Rev. Med. Virol. 11:2001;103-116.
4. Chambers P., Pringle C.R., Easton A.J. Heptad repeat sequences are located adjacent to hydrophobic regions in several types of virus fusion glycoproteins. J. Gen. Virol. 71:1990;3075-3080.
5. Chambers P., Pringle C.R., Easton A.J. Sequence analysis of the gene encoding the fusion glycoprotein of pneumonia virus of mice suggests possible conserved secondary structure elements in paramyxovirus fusion glycoproteins. J. Gen. Virol. 73:1992;1717-1724.
6. Chapman B.S., Thayer R.M., Vincent K.A., Haigwood N.L. Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells. Nucleic Acids Res. 19:1991;3979-3980.
7. Chen L., Gorman J.J., McKimm-Breschkin J., Lawrence L.J., Tulloch P.A., Smith B.J., Colman P.M., Lawrence M.C. The structure of the fusion glycoprotein of Newcastle disease virus suggests a novel paradigm for the molecular mechanism of membrane fusion. Structure. 9:2001;255-266.
8. Collins P.L., McIntosh K., Chanock R.M. Respiratory syncytial virus. Fields B.N., Knipe D.M., Howely P.M. Field's Virology. 3rd ed: 1996;Lippincott-Raven, Philadelphia.
9. Denizot F., Lang R. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability . J. Immunol. Methods. 89:1986;271-277.
10. Eckert D.M., Malashkevich V.N., Hong L.H., Carr P.A., Kim P.S. Inhibiting HIV-1 entry discovery of D-peptide inhibitors that target the gp41 coiled-coil pocket . Cell. 99:1999;103-115.
11. Eisenberg D., Luthy R., Bowie J.U. VERIFY3D assessment of protein models with three-dimensional profiles . Methods Enzymol. 277:1997;396-404.
12. Gonzalez-Reyes L., Begona Ruiz-Arguello M., Garcia-Barreno B., Cladér L., Lopez J.A., Albar J.P., Skehel J.J., Wiley D.C., Melero J.A. Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion. Proc. Natl. Acad. Sci. USA. 98:2001;9859-9864.
13. Groskreutz, D., Schenborn, E., 1994. Increased gene expression in mammalian cells lines using pAdVAntage DNA as a co-transfectant. Promega Notes Magazine 48, 8.
14. Guex N., Peitsch M.C. SWISS-MODEL and the Swiss-Pdb Viewe an environment for comparative protein modeling . Electrophoresis. 18:1997;2714-2723.
15. Haas J., Park E.C., Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6:1996;15-324.
16. Hallak L.K., Spillman D., Collins P.L., Peeples M.E. Glycosaminoglycan sulfation requirements for respiratory syncytial virus infection. J. Virol. 74:2000;10508-10513.
17. Heminway B.R., Yu Y., Tanaka Y., Perrine K.G., Gustafson E., Bernstein J.M., Galinski S. Analysis of respiratory syncytial virus F, G and SH protein in cell fusion. Virology. 200:1994;801-805.
18. Kahn J.S., Schnell M.J., Buonocore L., Rose J.K. Recombinant vesicular stomatitis virus expressing respiratory syncytial virus (RSV) glycoproteins RSV fusion protein can mediate infection and cell fusion . Virology. 254:1999;81-91.
19. Karger A., Schmidt U., Buchholz U.J. Recombinant bovine respiratory syncytial virus with deletions of the G and SH genes G and F proteins bind heparin . J. Gen. Virol. 82:2000;631-640.
20. Lawless-Delmedico M.K., Sista P., Sen R., Moore N.C., Antczak J.B., White J.M., Greene R.J., Leanza K.C., Matthews T.J., Lambert D.M. Heptad-repeat regions of respiratory syncytial virus F1 protein form a six-membered coiled-coil complex. Biochemistry. 39:2000;11684-11695.
21. Lopez J.A., Andreu D., Carreno C., Whyte P., Taylor G., Melero J.A. Conformational constraints of conserved neutralising epitopes from a major antigenic area of human respiratory syncyital virus fusion protein. J. Gen. Virol. 77:1996;649-660.
22. Lopez J.A., Bustos R., Orvell C., Berois M., Arbiza J., Garcia-Barreno B., Melero J.A. Antigenic structure of human respiratory syncytial virus fusion glycoprotein. J. Virol. 72:1998;6922-6928.
23. Martinez I., Melero J.A. Binding of human respiratory syncytial virus to cells implication of sulfated cell surface proteoglycans . J. Gen. Virol. 81:2000;2715-2722.
24. Mason, A., Young, P.R., Tucker, S.P., November 2000. Method of expression and agents identified thereby. U.S. Patent 60/262,767.
25. Matthews J.M., Young T.F., Tucker S.P., Mackay J.P. The core of the respiratory syncytial virus fusion protein is a trimeric coiled coil. J. Virol. 74:2000;5911-5920.
26. Monto A.S., Lim S.K. The Tecumseh study of respiratory illness. 3. Incidence and periodicity of respiratory syncytial virus and Mycoplasma pneumoniae infections. Am. J. Epidemiol. 94:1971;290-301.
27. Rost B., Sander C., Schneider R. PHD - an automatic mail server for protein secondary structure prediction. Comput. Appl. Biosci. 10:1994;53-60.
28. 4. Inorg. Nucl. Chem. Lett. 12:1976;247-250.
29. Smith B.J., Lawrence M.C., Colman P.M. Modelling the structure of the fusion protein from human respiratory syncytial virus. Protein Eng. 15:2002;365-371.
30. Techaarpornkul S., Barretto N., Peeples M.E. Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein. J. Virol. 75:2001;6825-6834.
31. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice . Nucleic Acids Res. 22:1994;4673-4680.
32. Toiron C., Lopez J.A., Rivas G., Andreu D., Melero J.A., Bruix M. Conformational studies of a short linear peptide corresponding to a major conserved neutralizing epitope of human respiratory syncytial virus fusion glycoprotein. Biopolymers. 39:1996;537-548.
33. Trudel M., Nadon F., Seguin C., Dionne G., Lacroix M. Identification of a synthetic peptide as a part of a major neutralization epitope of respiratory syncytial virus. J. Gen. Virol. 68:1987;2273-2280.
34. Watanabe W., Konno K., Ijichi K., Inoue H., Yokota T., Shigeta S. MTT colorimetric assay system for the screening of anti-orthomyxo- and anti-paramyxoviral agents. J. Virol. Methods. 48:1994;257-265.
35. Wyde P.R., Moore-Poveda D.K., O'Hara B., Ding W.-D., Mitsner B., Gilbert B.E. CL387626 exhibits marked and unusual antiviral activity against respiratory syncytial virus in tissue culture and in cotton rats. Antiviral Res. 38:1998;31-42.
36. Zhao X., Singh M., Malashkevich V.N., Kim P.S. Structural characterization of the human respiratory syncytial virus fusion protein core. Proc. Natl. Acad. Sci. USA. 97:2000;14172-14177.
37. Zhou G., Ferrer M., Chopra R., Kapoor T.M., Strassmaier T., Weissenhorn W., Skehel J.J., Oprian D., Schreiber S.L., Harrison S.C., Wiley D.C. The structure of an HIV-1 specific cell entry inhibitor in complex with the HIV-1 gp41 trimeric core. Bioorg. Med. Chem. 8:2000;2219-2227.
38. Zimmer G., Budz L., Herrler G. Proteolytic activation of respiratory syncytial virus fusion protein cleavage at two furin consensus sequences . J. Biol. Chem. 276:2001;31642-31650.