Focal adhesion kinase N-terminus in breast carcinoma cells induces rounding, detachment and apoptosis

Biochemical Journal

Volumn 373, Issue 1, 2003, Pages 201-210

  Beviglia, Lucia ^a,c   Golubovskaya, Vita ^a   Xu, LiHui ^a   Yang, XiHui ^a   Craven, Rolf J ^a,d   Cance, William G ^b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

^c CELERA   (United States)

^d UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Apoptosis; Breast cancer cell; Caspase activation; Protein degradation

Indexed keywords

ADHESION; CELLS; DEGRADATION; PROTEINS;

FOCAL ADHESION KINASE (FAK);

ENZYMES;

CASPASE 3; FOCAL ADHESION KINASE; TYROSINE;

AMINO TERMINAL SEQUENCE; APOPTOSIS; ARTICLE; BREAST CARCINOMA; CELL ADHESION; CELL STRAIN MCF 7; CELL SURVIVAL; CONTROLLED STUDY; ENZYME ACTIVATION; EPITHELIUM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DEPHOSPHORYLATION; PROTEIN DOMAIN; PROTEIN EXPRESSION;

APOPTOSIS; BASE SEQUENCE; BREAST NEOPLASMS; CASPASE 3; CASPASES; CELL SIZE; DNA PRIMERS; DNA, COMPLEMENTARY; ENZYME ACTIVATION; FEMALE; FOCAL ADHESION KINASE 1; FOCAL ADHESION PROTEIN-TYROSINE KINASES; HUMANS; PEPTIDE FRAGMENTS; POLYMERASE CHAIN REACTION; PROTEIN-TYROSINE KINASES; RECOMBINANT FUSION PROTEINS; TRANSFECTION; TUMOR CELLS, CULTURED;

MINK CELL FOCUS-FORMING VIRUS;

EID: 0038005805     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021846     Document Type: Article

References (51)

1. FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. U.S.A. 89, 5192-5196
2. Zachary, I. (1997) Focal adhesion kinase. Int. J. Biochem. Cell Biol. 29, 929-934
3. Kanner, S. B., Reynolds, A. B., Vines, R. R. and Parsons, J. T. (1990) Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases Proc. Natl. Acad. Sci. U.S.A. 87, 3328-3332
4. FAK. Mol. Cell. Biol. 14, 147-155
5. Kornberg, L. J., Earp, H. S., Turner, C. E., Prockop, C. and Juliano, R. L. (1991) Signal transduction by integrins: increased protein tyrosine phosphorylation caused by clustering of β1 integrins. Proc. Natl. Acad. Sci. U.S.A. 88, 8392-8398
6. FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119, 893-903
7. Cary, L. A. and Guan, J. L. (1999) Focal adhesion kinase in integrin-mediated signaling. Front. Biosci. 4, D102-D113
8. FAK): a point of convergence in the action of neuropeptides, integrins, and oncogenes. Cell (Cambridge, Mass.) 71, 891-894
9. FAK) and paxillin tyrosine phosphorylation in Swiss 3T3 cells. Bell-shaped dose response and cross-talk with bombesin. J. Biol. Chem. 269, 704-710
10. FAK) and promotes migration and invasion by oral squamous cell carcinoma cells. J. Biol. Chem. 269, 31807-31813
11. Beviglia, L. and Kramer, R. H. (1999) HGF induces FAK activation and integrin-mediated adhesion in MTLn3 breast carcinoma cells. Int. J. Cancer 83, 640-649
12. Chen, H. C. and Guan, J. L. (1994) Association of focal adhesion kinase with its potential substrate phosphatidylinositol 3-kinase. Proc. Natl. Acad. Sci. U.S.A. 91, 10148-10152
13. Cas. Proc. Natl. Acad. Sci. U.S.A. 92, 10678-10682
14. cas, a substrate associated with v-Src and v-Crk, localizes to focal adhesions and binds to focal adhesion kinase. J. Biol. Chem. 271, 13649-13655
15. Schlaepfer, D. D., Hanks, S. K., Hunter, T. and van der Geer, P. (1994) Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature (London) 372, 786-791
16. Schlaepfer, D. D. and Hunter, T. (1996) Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases. Mol. Cell. Biol. 16, 5623-5633
17. Weiner, T. M., Liu, E. T., Craven, R. J. and Cance, W. G. (1994) Expression of growth factor receptors, the focal adhesion kinase, and other tyrosine kinases in human soft tissue tumors. Ann. Surg. Oncol. 1, 18-27
18. Maung, K., Easty, D. J., Hill, S. P. and Bennett, D. C. (1999) Requirement for focal adhesion kinase in tumor cell adhesion. Oncogene 18, 6824-6828
19. Agochiya, M., Brunton, V. G., Owens, D. W., Parkinson, E. K., Paraskeva, C., Keith, W. N. and Frame, M. C. (1999) Increased dosage and amplification of the focal adhesion kinase gene in human cancer cells. Oncogene 18, 5646-5653
20. Xu, L. H., Yang, X., Craven, R. J. and Cance, W. G. (1998) The COOH-terminal domain of the focal adhesion kinase induces loss of adhesion and cell death in human tumor cells. Cell. Growth. Differ. 9, 999-1005
21. Frisch, S. M., Vuori, K., Ruoslahti, E. and Chan-Hui, P. Y. (1996) Control of adhesion-dependent cell survival by focal adhesion kinase. J. Cell Biol. 134, 793-799
22. FAK in cultured fibroblasts results in apoptosis. J. Cell Biol. 135, 1383-1390
23. Ilic, D., Almeida, E. A., Schlaepfer, D. D., Dazin, P., Aizawa, S. and Damsky, C. H. (1998) Extracellular matrix survival signals transduced by focal adhesion kinase suppress p53-mediated apoptosis. J. Cell Biol. 143, 547-560
24. IIIc, D., Furuta, Y., Kanazawa, S., Takeda, N., Sobue, K., Nakatsuji, N., Nomura, S., Fujimoto, J., Okada, M. and Yamamoto, T. (1995) Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature (London) 377, 539-544
25. Cary, L. A., Chang, J. F. and Guan, J. L. (1998) Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn. J. Cell Sci. 109, 1787-1794
26. Nolan, K., Lacoste, J. and Parsons, J. T. (1999) Regulated expression of focal adhesion kinase-related nonkinase, the autonomously expressed C-terminal domain of focal adhesion kinase. Mol. Cell. Biol. 19, 6120-6129
27. FAK. Mol. Cell. Biol. 13, 785-791
28. FAK, to cellular focal adhesions. J. Cell Biol. 123, 993-1005
29. FAK. Nature (London) 380, 538-540
30. Xu, L.-h., Yang, X.-h., Bradham, C. A., Brenner, D. A., Baldwin, A. S., Craven, R. J. and Cance, W. G. (2000) The focal adhesion kinase suppresses transformation-associated, anchorage-independent apoptosis in human breast cancer cells. J. Biol. Chem. 275, 30597-30604
31. Golubovskaya, V., Beviglia, L., Xu, L. H., Earp, III, H. S., Craven, R. and Cance, W. (2002) Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells. J Biol. Chem. 277, 38978-38987
32. Schaller, M. D., Otey, C. A., Hildebrand, J. D. and Parsons, J. T. (1995) Focal adhesion kinase and paxillin bind to peptides mimicking beta integrin cytoplasmic domains. J. Cell. Biol. 130, 1181-1187
33. Horwitz, A., Duggan, K, Buck, C., Beckerle, M. C. and Burridge, K. (1986) Interaction of plasma membrane fibronectin receptor with talin - a transmembrane linkage. Nature (London) 320, 531-533
34. src. Mol. Cell. Biol. 14, 1680-1688
35. Chen, H. C., Appeddu, P. A., Isoda, H. and Guan, J. L. (1996) Phosphorylation of tyrosine 397 in focal adhesion kinase is required for binding phosphatidylinositol 3-kinase. J. Biol. Chem. 271, 26329-26334
36. Thomas, J. W., Ellis, B., Boemer, R. J., Knight, W. B., White, II, G. C. and Schaller, M. D. (1998) SH2- and SH3-mediated interactions between focal adhesion kinase and Src. J. Biol. Chem. 273, 577-583
37. Han, D. C. and Guan, J. L. (1999) Association of focal adhesion kinase with Grb7 and its role in cell migration. J. Biol. Chem. 274, 24425-24430
38. FAK. Mol. Cell. Biol. 15, 2819-2827
39. 2-terminal FAK in the nuclei of apoptotic glioblastoma cells. Cancer Res 61, 4978-4981
40. Lobo, M. and Zachary, I. (2000) Nuclear localization and apoptotic regulation of an N-terminal domain focal adhesion kinase fragment in endothelial cells. Biochem. Biophys. Res. Commun. 276, 1068-1074
41. Stewart, A., Ham, C. and Zachary, I. (2002) The focal adhesion kinase N-terminal domain localises to nuclei and intercellular junctions in HEK 293 and MDCK ceils independently of tyrosine 397 and the C-terminal domain. Biochem. Biophys. Res. Commun. 299, 62-73
42. Xu, L. H., Owens, L. V., Sturge, G. C., Yang, X., Liu, E. T., Craven, R. J. and Cance, W. G. (1996) Attenuation of the expression of the focal adhesion kinase induces apoptosis in tumor cells. Cell. Growth Differ 7, 413-418
43. FAK protein tyrosine kinase in hydrogen peroxide-induced apoptosis of T98G cells. Biochem. Biophys. Res. Commun. 241, 769-774
44. van De Water, B., Houtepen, F., Huigsloot, M. and Tijdens, I. B. (2001) Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury. J. Biol. Chem. 276, 36183-36193
45. Gilmore, A. P. and Romer, L. H. (1996) Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation. Mol. Biol. Cell 7, 1209-1224
46. Frisch, S. M. and Francis, H. (1994) Disruption of epithelial cell-matrix interactions induces apoptosis. J. Cell Biol. 124, 619-626
47. Richardson, A., Malik, R. K., Hildebrand, J. D. and Parsons, J. T. (1997) Inhibition of cell spreading by expression of the C-terminal domain of focal adhesion kinase (FAK) is rescued by coexpression of Src or catalytically inactive FAK: a role for paxillin tyrosine phosphorylation. Mol. Cell. Biol. 17, 6906-6914
48. Zhang, X., Chattopadhyay, A., Ji, Q. S., Owen, J. D., Ruest, P. J., Carpenter, G. and Hanks, S. K. (1999) Focal adhesion kinase promotes phospholipase C-γ1 activity. Proc. Natl. Acad. Sci. U.S.A. 96, 9021-9026
49. Sieg, D. J., Hauck, C. R. and Schlaepfer, D. D. (1999) Required role of focal adhesion kinase (FAK) for integrin-stimulated cell migration. J. Cell Sci. 112, 2877-2891
50. Dunty, J. M. and Schaller, M. D. (2002) The N termini of focal adhesion kinase family members regulate substrate phosphorylation, localization, and cell morphology. J. Biol. Chem. 277, 45844-45854
51. Martin, S. S. and Leder, P. (2001) Human MCF10A mammary epithelial cells undergo apoptosis following actin depolymerization that is independent of attachment and rescued by Bcl-2. Mol. Cell. Biol. 21, 6529-6536