Presteady-state and steady-state kinetics and turnover rate of the mouse γ-aminobutyric acid transporter (mGAT3)

Journal of Membrane Biology

Volumn 190, Issue 1, 2002, Pages 57-73

  Sacher, A ^a   Nelson, N ^a   Ogi, J T ^b   Wright, E M ^c   Loo, D D F ^c   Eskandari, S ^b  

^a TEL AVIV UNIVERSITY   (Israel)

^b CALIFORNIA STATE POLYTECHNIC UNIVERSITY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Charge movement; Chloride dependence; GABA; Mouse GAT3; Neurotransmitter transporter

Indexed keywords

4 AMINOBUTYRIC ACID; CHLORIDE ION; COMPLEMENTARY RNA; EXCITATORY AMINO ACID TRANSPORTER; SODIUM ION;

ANIMAL CELL; ARTICLE; GENE EXPRESSION; KINETICS; MEMBRANE ELECTROPHYSIOLOGY; MEMBRANE TRANSPORT; MOLECULAR CLONING; MOUSE; NEUROTRANSMISSION; NONHUMAN; OOCYTE; STEADY STATE; SYNAPSE; XENOPUS;

ANIMALS; CELLS, CULTURED; CLONING, MOLECULAR; FEMALE; GABA PLASMA MEMBRANE TRANSPORT PROTEINS; HOMEOSTASIS; KINETICS; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT PROTEINS; MICE; OOCYTES; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; XENOPUS LAEVIS;

ANIMALIA; XENOPUS LAEVIS;

EID: 0037997484     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-002-1024-6     Document Type: Article

References (65)

1. Borden, L.A. 1996. GABA transporter heterogeneity: Pharmacology and cellular localization. Neurochem. Int. 29:335-356
2. Borden, L.A., Dhar, T.G., Smith, K.E., Branchek, T.A., Gluchowski, C., Weinshank, R.L. 1994. Cloning of the human homologue of the GABA transporter GAT-3 and identification of a novel inhibitor with selectivity for this site. Receptors Channels 2:207-213
3. Borden, L.A., Smith, K.E., Gustafson, E.L., Branchek, T.A., Weinshank, R.L. 1995. Cloning and expression of a betaine/GABA transporter from human brain. J. Neurochem. 64:977-984
4. Borden, L.A., Smith, K.E., Hartig, P.R., Branchek, T.A., Weinshank, R.L. 1992. Molecular heterogeneity of the γ-aminobutyric acid (GABA) transport system. Cloning of two novel high affinity GABA transporters from rat brain. J. Biol. Chem. 267:21098-21104
5. Cammack, J.N., Schwartz, E.A. 1996. Channel behavior in a γ-aminobutyrate transporter. Proc. Natl. Acad. Sci. USA 93:723-727
6. Cammack, J.N., Rakhilin, S.V., Schwartz, E.A. 1994. A GABA transporter operates asymmetrically and with variable stoichiometry. Neuron 13:949-960
7. Cao, Y., Li, M., Mager, S., Lester, H.A. 1998. Amino acid residues that control pH modulation of transport-associated current in mammalian serotonin transporters. J. Neurosci. 18:7739-7749
8. Clark, J.A., Amara, S.G. 1994. Stable expression of a neuronal γ-aminobutyric acid transporter, GAT-3, in mammalian cells demonstrates unique pharmacological properties and ion dependence. Mol. Pharmacol. 46:550-557
9. Clark, J.A., Deutch, A.Y., Gallipoli, P.Z., Amara, S.G. 1992. Functional expression and CNS distribution of a β-alanine-sensitive neuronal GABA transporter. Neuron 9:337-348
10. Eskandari, S., Kreman, M., Kavanaugh, M.P., Wright, E.M., Zampighi, G.A. 2000. Pentameric assembly of a neuronal glutamate transporter. Proc. Natl. Acad. Sci. USA. 97:8641-8646
11. - symporter. Mechanism, stoichiometry, and specificity. J. Biol. Chem. 272:27230-27238
12. Fairman, W.A., Vandenberg, R.J., Arriza, J.L., Kavanaugh, M.P., Amara, S.G. 1995. An excitatory amino-acid transporter with properties of a ligand-gated chloride channel. Nature 375:599-603
13. Forlani, G., Bossi, E., Ghirardelli, R., Giovannardi, S., Binda, F., Bonadiman, L., Ielmini, L., Peres, A. 2001a. Mutation K448E in the external loop 5 of rat GABA transporter rGAT1 induces pH sensitivity and alters substrate interactions. J. Physiol. 536:479-494
14. Forlani, G., Bossi, E., Perego, C., Giovannardi, S., Peres, A. 2001b. Three kinds of currents in the canine betaine-GABA transporter BGT-1 expressed in Xenopus laevis oocytes. Biochim. Biophys. Acta 1538:172-180
15. i-2). J. Gen. Physiol. 112:1-18
16. i-5) expressed in Xenopus Jaevis oocytes. J. Membrane Biol. 160:9-25
17. Gadea, A., López-Colomé, A.M. 2001. Glial transporters for glutamate, glycine, and GABA: II. GABA transporters. J. Neurosci. Res. 63:461-468
18. Galli, A., Blakely, R.D., DeFelice, L.J. 1996. Norepinephrine transporters have channel modes of conduction. Proc. Natl. Acad. Sci. USA 93:8671-8676
19. Galli, A., DeFelice, LJ., Duke, B.-J., Moore, K.R., Blakely, R.D. 1995. Sodium-dependent norepinephrine-induced currents in norepinephrine-transporter-transfected HEK-293 cells blocked by cocaine and antidepressants. J. Exp. Biol. 198:2197-2212
20. Green, A.R., Hainsworth, A.H., Jackson, D.M. 2000. GABA potentiation: A logical pharmacological approach for the treatment of acute ischaemic stroke. Neuropharmacology 39:1483-1494
21. Guastella, J., Nelson, N., Nelson, H., Czyzyk, L., Keynan, S., Miedel, M.C., Davidson, N., Lester, H.A., Kanner, B.I. 1990. Cloning and expression of a rat brain GABA transporter. Science 249:1303-1306
22. +/glucose cotransporter(SGLTI). J. Membrane Biol. 155:175-186
23. -) cotransport function. Database reconstruction with an alternating access model. J. Gen. Physiol. 114:459-475
24. +/glucose cotransporter expression by protein kinases in Xenopus laevis oocytes. J. Biol. Chem. 271:14740-14746
25. Jursky, F., Nelson, N. 1999. Developmental expression of the neurotransmitter transporter GAT3. J. Neurosci. Res. 55:394-399
26. Kavanaugh, M.P., Arriza, J.L., North, R.A., Amara, S.G. 1992. Electrogenic uptake of γ-aminobutyric acid by a cloned transporter expressed in Xenopus oocytes. J. Biol. Chem. 267:22007-22009
27. Keynan, S., Suh, Y.-J., Kanner, B.L, Rudnick, G. 1992. Expression of a cloned γ-aminobutyric acid transporter in mammalian cells. Biochemistry 31:1974-1979
28. Läuger, P. 1991. Electrogenic Ion Pumps. Sinauer Associates, Inc., Sunderland
29. Lester, H.A., Cao, Y., Mager, S. 1996. Listening to neurotransmitter transporters. Neuron 17:807-810
30. Lester, H.A., Mager, S., Quick, M.W., Corey J.L. 1994. Permeation properties of neurotransmitter transporters. Annu. Rev. Pharmacol. Toxicol. 34:219-249
31. Li, M., Farley, R.A., Lester, H.A. 2000. An intermediate state of the γ-aminobutyric acid transporter GAT1 revealed by simultaneous voltage clamp and fluorescence. J. Gen. Physiol. 115:491-508
32. - neurotransmitter transporters. Methods Enzymol. 296:425-436
33. + channel determined by construction of multimeric cDNAs. Neuron 9:861-871
34. Lin, F., Lester, H.A., Mager, S. 1996. Single-channel currents produced by the serotonin transporter and analysis of a mutation affecting ion permeation. Biophys. J. 71:3126-3135
35. Liu, Q.-R., López-Corcuera, B., Mandiyan, S., Nelson, H., Nelson, N. 1993. Molecular characterization of four pharmacologically distinct γ-aminobutyric acid transporters in mouse brain. J. Biol. Chem. 268:2106-2112
36. Liu, Q.-R., Mandiyan, S., Nelson, H., Nelson, N. 1992. A family of genes encoding neurotransmitter transporters. Proc. Natl. Acad. Sci. USA 89:6639-6643
37. +-coupled cotransporters GAT1 and SGLT1. J. Biol. Chem. 275:37414-37422
38. +/glucose cotransporter. Proc. Natl. Acad. Sci. USA 90:5767-5771
39. +/and glucose transport. Proc. Natl. Acad. Sci. USA 95:7789-7794
40. Loo, D.D.F., Hirayama, B.A., Meinild, A.-K., Chandy, G., Zeuthen, T., Wright, E.M. 1999. Passive water and ion transport by cotransporters. J. Physiol. 518:195-202
41. López-Corcuera, B., Liu, Q.-R., Mandiyan, S., Nelson, H., Nelson, N. 1992. Expression of a mouse brain cDNA encoding novel γ-aminobutyric acid transporter. J. Biol. Chem. 267:17491-17493
42. López-Corcuera, B., Martínez-Maza, R., Núñez, E., Roux, M., Supplisson, S., Aragón, C. 1998. Differential properties of two stably expressed brain-specific glycine transporters. J. Neurochem. 71:2211-2219
43. -) cotransport function. Steady state studies in giant Xenopus oocyte membrane patches. J. Gen. Physiol. 114:429-444
44. -) cotransport function. Kinetic studies in giant Xenopus oocyte membrane patches. J. Gen. Physiol. 114:445-457
45. Lu, C.-C, Kabakov, A., Markin, V.S., Mager, S., Frazier, G.A., Hilgemann, D.W. 1995. Membrane transport mechanisms probed by capacitance measurements with megahertz voltage clamp. Proc. Natl. Acad. Sci. USA 92:11220-11224
46. +-coupled oligopeptide transporter hPEPT1. J. Biol. Chem. 271:5430-5437
47. +/glucose cotransporter (SGLT1) currents and fluxes. J. Membrane Biol. 162:101-106
48. Mager, S., Kleinberger-Doron, N., Keshet, G.I., Davidson, N., Kanner, B.I., Lester, H.A. 1996. Ion binding and permeation at the GABA transporter GAT1. J. Neurosci. 16:5405-5414
49. Mager, S., Min, C., Henry, D.J., Chavkin, C., Hoffman, B.J., Davidson, N., Lester, H.A. 1994. Conducting states of a mammalian serotonin transporter. Neuron 12:845-859
50. Mager, S., Naeve, J., Quick, M., Labarca, C., Davidson, N., Lester, H.A. 1993. Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes. Neuron 10:177-188
51. Matskevitch, I., Wagner, C.A., Stegen, C., Bröer, S., Noll, B., Risler, T., Kwon, H.M., Handler, J.S., Waldegger, S., Busch, A.E., Lang, F. 1999. Functional characterization of the betaine/γ-aminobutyric acid transporter BGT-1 expressed in Xenopus oocytes. J. Biol. Chem. 274:16709-16716
52. - neurotransmitter transporters. J. Neurochem. 71:1785-1803
53. Nelson, H., Mandiyan, S., Nelson, N. 1990. Cloning of the human brain GABA transporter. FEBS Lett. 269:181-184
54. +/glucose cotransporter SGLT1. Biochemistry 37:10522-10528
55. Pastuszko, A., Wilson, D.F., Erecinska, M. 1982. Energetics of γ-aminobutyrate transport in rat brain synaptosomes. J. Biol. Chem. 257:7514-7519
56. Reizer, J., Reizer, A., Saier, M.H. Jr. 1994. A functional superfamily of sodium/solute symporters. Biochim. Biophys. Acta 1197:133-166
57. Roettger, V.R., Amara, S.G. 1999. GABA and glutamate transporters: Therapeutic and etiologic implications for epilepsy. Adv. Neurol. 79:551-560
58. Schachter, S.C. 1999. A review of the antiepileptic drug tiagabine. Clin. Neuropharmacol. 22:312-317
59. Sonders, M.S., Amara, S.G. 1996. Channels in transporters. Curr. Opin. Neurobiol. 6:294-302
60. Sonders, M.S., Zhu, S.-J., Zahniser, N.R., Kavanaugh, M.P., Amara, S.G. 1997. Multiple ionic conductances of the human dopamine transporter: The actions of dopamine and psychostimulants. J. Neurosci. 17:960-974
61. +/glucose cotransporter expressed in Xenogus oocytes is electrogenic. Biophys. J. 57:1217-1224
62. Wadiche, J.I., Arriza, J.L., Amara, S.G., Kavanaugh, M.P. 1995. Kinetics of a human glutamate transporter. Neuron 14:1019-1027
63. +/glucose cotransporter. Acta Physiol. Scand. 163 (Suppl. 643):257-264
64. --dependent betaine transporter that is regulated by hypertonicity. J. Biol. Chem. 267:649-652
65. Zampighi, G.A., Kreman, M., Boorer, K.J., Loo, D.D.F., Bezanilla, F., Chandy, G., Hall, J.E., Wright, E.M. 1995. A method for determining the unitary functional capacity of cloned channels and transporters expressed in Xenopus laevis oocytes. J. Membrane Biol. 148:65-78