GAT1 (GABA:Na+:Cl-) cotransport function: Kinetic studies in giant Xenopus oocyte membrane patches

Journal of General Physiology

Volumn 114, Issue 3, 1999, Pages 445-457

  Lu, Chin Chih ^a   Hilgemann, Donald W ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Charge movement; Neurotransmitter transporter; NO 711; Transport kinetics; Voltage dependence

Indexed keywords

CHLORIDE ION; MEMBRANE PROTEIN; SODIUM ION;

ARTICLE; CONFORMATIONAL TRANSITION; MEMBRANE BINDING; MEMBRANE POTENTIAL; MEMBRANE TRANSPORT; NONHUMAN; SODIUM TRANSPORT; STEADY STATE; TRANSPORT KINETICS; XENOPUS;

ALGORITHMS; ANIMALS; CARRIER PROTEINS; CELL MEMBRANE; CHLORIDES; CYTOPLASM; ELECTROPHYSIOLOGY; EXTRACELLULAR SPACE; GABA PLASMA MEMBRANE TRANSPORT PROTEINS; GAMMA-AMINOBUTYRIC ACID; KINETICS; MEMBRANE POTENTIALS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; OOCYTES; ORGANIC ANION TRANSPORTERS; PATCH-CLAMP TECHNIQUES; SODIUM; SODIUM CHLORIDE SYMPORTERS; SYMPORTERS; XENOPUS LAEVIS;

EID: 0032886824     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.114.3.445     Document Type: Article

References (25)

1. +-glucose cotransporter. Biophys. J. 71:2544-2552.
2. Gadsby, D.C., R.F. Rakowski, and P. De Weer. 1993. Extracellular access to the Na,K pump: pathway similar to ion channel. Science. 260:100-103.
3. 2+ exchange in giant excised sarcolemmal membrane patches. Nature. 344:242-245.
4. 2+ exchanger. Nature. 352:715-718.
5. Hilgemann, D.W. 1994. Channel-like function of the Na,K pump probed at microsecond resolution in giant membrane patches. Science. 263:1429-1432.
6. 2+ exchange current magnitudes determined from channel-like noise and charge movements of ion transport. Biophys. J. 71:759-768.
7. Hilgemann, D.W. 1997. Recent electrical snapshots of the cardiac Na,K pump. Ann. NY Acad. Sci. 834:260-269.
8. -) cotransport function: database reconstruction with an alternating access model. J. Gen. Physiol. 114:459-475.
9. Kavanaugh, M.P., J.L. Arriza, R.A. North, and S.G. Amara. 1992. Electrogenic uptake of γ-aminobutyric acid by a cloned transporter expressed in Xenopus oocytes. J. Biol. Chem. 267:22007-22009.
10. Keynan, S., and B.I. Kanner. 1988. γ-Aminobutyric acid transport in reconstituted preparations from rat brain: coupled sodium and chloride fluxes. Biochemistry. 27:12-17.
11. Lagnado, L., and P.A. McNaughton. 1990. Electrogenic properties of the Na:Ca exchange. J. Membr. Biol. 113:177-191.
12. +-pump: microscopic analysis of nonstationary ion-translocation. Biochim. Biophys. Acta. 944:451-464.
13. Läuger, P. 1991. Electrogenic Ion Pumps. Sinauer Associates, Inc., Sunderland, MA. 74-81.
14. +/glucose cotransporter. Proc. Natl. Acad. Sci. USA. 90:5767-5771.
15. -) cotransport function: steady state studies in giant Xenopus oocyte membrane patches. J. Gen. Physiol 114:429-444.
16. Lu, C.C., A. Kabakov, V.S. Markin, S. Mager, G.A. Frazier, and D.W. Hilgemann. 1995. Membrane transport mechanisms probed by capacitance measurements with megahertz voltage clamp. Proc. Natl. Acad. Sci. USA. 92:11220-11224.
17. Mager, S., N. Kleinberger-Doron, G.I. Keshet, N. Davidson, B.I. Kanner, and H.A. Lester. 1996. Ion binding and permeation at the GABA transporter GAT1. J. Neurosci. 16:5405-5414.
18. Mager, S., J. Naeve, M. Quick, C. Labarca, N. Davidson, and H.A. Lester. 1993. Steady-states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes. Neuron. 10:177-188.
19. Nakao, M., and D.C. Gadsby. 1986. Voltage dependence of Na translocation by the Na/K pump. Nature. 323:628-630.
20. +/glucose cotransporter: II. A transport model under nonrapid equilibrium conditions [published erratum appears in J. Membr. Biol. 1992. 130:203]. J. Membr. Biol. 125:63-79.
21. Radian, R., and B.I. Kanner. 1983. Stoichiometry of sodium-and chloride-coupled γ-aminobutyric acid transport by synaptic plasma membrane vesicles isolated from rat brain. Biochemistry. 22:1236-1241.
22. Rakowski, R.F. 1993. Charge movement by the Na/K pump in Xenopus oocytes. J. Gen. Physiol. 101:117-144.
23. Rakowski, R.F., D.C. Gadsby, and P. De Weer. 1997. Voltage dependence of the Na/K pump. J. Membr. Biol. 155:105-112.
24. Sturmer, W., R. Buhler, H.J. Apell, and P. Länger. 1991. Charge translocation by the Na,K-pump: II. Ion binding and release at the extracellular face. J. Membr. Biol. 121:163-176.
25. Wadiche, J.I., J.L. Arriza, S.G. Amara, and M.P. Kavanaugh. 1995. Kinetics of a human glutamate transporter. Neuron. 14:1019-1027.