Comparison of the comet assay and the oxygen microelectrode for measuring tumor oxygenation in head-and-neck cancer patients

International Journal of Radiation Oncology Biology Physics

Volumn 56, Issue 2, 2003, Pages 375-383

  Le, Quynh Thu ^a   Kovacs, Mary S ^a   Dorie, Mary Jo ^a   Koong, Albert ^a   Terris, David J ^a   Pinto, Harlan A ^b   Goffinet, Don R ^a   Nowels, Kent ^b   Bloch, Dan ^a   Brown, J Martin ^a  

^a STANFORD UNIVERSITY   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Comet assay; Eppendorf microelectrode; Head and neck cancer; Hypoxia; Tumor oxygenation

Indexed keywords

CARCINOGENS; DNA; MICROELECTRODES; STATISTICAL METHODS; TUMORS;

HISTOGRAPHS;

ONCOLOGY;

DNA; OXYGEN;

ADULT; ALKALINITY; ANALYTIC METHOD; ARTICLE; CLINICAL TRIAL; COMET ASSAY; CORRELATION ANALYSIS; DNA DAMAGE; DNA STRAND BREAKAGE; FEMALE; GEL ELECTROPHORESIS; HEAD AND NECK CARCINOMA; HISTOGRAM; HUMAN; HYPOXIA; IN VITRO STUDY; IN VIVO STUDY; INTERMETHOD COMPARISON; IRRADIATION; MAJOR CLINICAL STUDY; MALE; METASTASIS; MICROELECTRODE; OXYGENATION; PRIORITY JOURNAL; SQUAMOUS CELL CARCINOMA; STATISTICAL SIGNIFICANCE; TIME; TUMOR CELL;

EID: 0037963317     PISSN: 03603016     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0360-3016(02)04503-0     Document Type: Article

References (26)

1. Brown J.M., Giaccia A.J. The unique physiology of solid tumors Opportunities (and problems) for cancer therapy . Cancer Res. 58:1998;1408-1416.
2. Brizel D.M., Dodge R.K., Clough R.W., et al. Oxygenation of head and neck cancer Changes during radiotherapy and impact on treatment outcome . Radiother Oncol. 53:1999;113-117.
3. Rudat V., Vanselow B., Wollensack P., et al. Repeatability and prognostic impact of the pretreatment pO(2) histography in patients with advanced head and neck cancer. Radiother Oncol. 57:2000;31-37.
4. Nordsmark M., Overgaard J. A confirmatory prognostic study on oxygenation status and loco-regional control in advanced head and neck squamous cell carcinoma treated by radiation therapy. Radiother Oncol. 57:2000;39-43.
5. Brizel D.M., Scully S.P., Harrelson J.M. Tumor oxygenation predicts for likelihood of distant metastasis in human soft tissue sarcoma. Cancer Res. 56:1996;941-943.
6. Hockel M., Schlenger K., Hockel S., et al. Hypoxic cervical cancers with low apoptotic index are highly aggressive. Cancer Res. 59:1999;4525-4528.
7. Fyles A., Milosevic M., Hedley D., et al. Tumor hypoxia has independent predictor impact only in patients with node-negative cervix cancer. J Clin Oncol. 20:2002;680-687.
8. Chia S.K., Wykoff C.C., Watson P.H., et al. Prognostic significance of a novel hypoxia-regulated marker, carbonic anhydrase IX, in invasive breast carcinoma. J Clin Oncol. 19:2001;3660-3668.
9. Aebersold D.M., Burri P., Beer K.T., et al. Expression of hypoxia-inducible factor-1alpha A novel predictive and prognostic parameter in the radiotherapy of oropharyngeal cancer . Cancer Res. 61:2001;2911-2916.
10. Fyles A.W., Milosevic M., Wong R., et al. Oxygenation predicts radiation response and survival in patients with cervix cancer. Radiother Oncol. 48:1998;149-156.
11. Aquino-Parsons C., Luo C., Vikse C.M., et al. Comparison between the comet assay and the oxygen microelectrode for measurement of tumor hypoxia. Radiother Oncol. 51:1999;179-185.
12. Kavanagh M.C., Tsang V., Chow S., et al. A comparison in individual murine tumors of techniques for measuring oxygen levels. Int J Radiat Oncol Biol Phys. 44:1999;1137-1146.
13. Gabalski E.C., Adam M., Pinto H., et al. Pretreatment and midtreatment measurement of oxygen tension levels in head and neck cancers. Laryngoscope. 108:1998;1856-1860.
14. Olive P.L., Durand R.E. Detection of hypoxic cells in a murine tumor with the use of the comet assay. Natl Cancer Inst. 84:1992;707-711.
15. Zhang H., Koch C.J., Wallen C.A., et al. Radiation-induced DNA damage in tumors and normal tissues. III. Oxygen dependence of the formation of strand breaks and DNA-protein crosslinks. Radiat Res. 142:1995;163-168.
16. Gehan E.A., Tefft M.C. Will there be resistance to the RECIST (Response Evaluation Criteria in Solid Tumors)? J Natl Cancer Inst. 92:2000;179-181.
17. Dorie M.J., Kovacs M.S., Gabalski E.C., et al. DNA damage measured by the comet assay in head and neck cancer patients treated with tirapazamine. Neoplasia. 1:1999;461-467.
18. Nordsmark M., Overgaard M., Overgaard J. Pretreatment oxygenation predicts radiation response in advanced squamous cell carcinoma of the head and neck. Radiother Oncol. 41:1996;31-39.
19. Olive P.L., Durand R.E., Jackson S.M., et al. The comet assay in clinical practice. Acta Oncol. 38:1999;839-844.
20. Robson T., Price M.E., Moore M.L., et al. Increased repair and cell survival in cells treated with DIR1 antisense oligonucleotides Implications for induced radioresistance . Int J Radiat Biol. 76:2000;617-623.
21. Wouters B.G., Brown J.M. Cells at intermediate oxygen levels can be more important than the"hypoxic fraction" in determining tumor response to fractionated radiotherapy. Radiat Res. 147:1997;541-550.
22. Nordsmark M., Loncaster J., Chou S.C., et al. Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma. Int J Radiat Oncol Biol Phys. 49:2001;581-586.
23. Jenkins W.T., Evans S.M., Koch C.J. Hypoxia and necrosis in rat 9L glioma and Morris 7777 hepatoma tumors Comparative measurements using EF5 binding and the Eppendorf needle electrode . Int J Radiat Oncol Biol Phys. 46:2000;1005-1017.
24. Loncaster J.A., Harris A.L., Davidson S.E., et al. Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia Correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix . Cancer Res. 61:2001;6394-6399.
25. Khalil A.A., Horsman M.R., Overgaard J. The importance of determining necrotic fraction when studying the effect of tumour volume on tissue oxygenation. Acta Oncol. 34:1995;297-300.
26. Terris D. Head and neck cancer The importance of oxygen . Laryngoscope. 110:2000;697-707.