Comparison of different techniques of quantitative PCR for determination of Streptococcus mutans counts in saliva samples

Oral Microbiology and Immunology

Volumn 18, Issue 1, 2003, Pages 50-53

  Rupf, S ^a   Merte, K ^a   Kneist, S ^b   Al Robaiy, S ^a   Eschrich, Klaus ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Competitive PCR; Melting curve analysis; Real time PCR; Streptococcus mutans

Indexed keywords

DNA;

ARTICLE; BACTERIUM DETECTION; CHILD; CLINICAL ARTICLE; DENTAL CARIES; HUMAN; INTERMETHOD COMPARISON; MOUTH CAVITY; NONHUMAN; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SALIVA ANALYSIS; STREPTOCOCCUS; STREPTOCOCCUS MUTANS;

BACTERIOLOGICAL TECHNIQUES; CHILD; COLONY COUNT, MICROBIAL; DENTAL CARIES; DENTAL CARIES ACTIVITY TESTS; DNA, BACTERIAL; HUMANS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SALIVA; SENSITIVITY AND SPECIFICITY; STATISTICS, NONPARAMETRIC; STREPTOCOCCUS MUTANS;

EID: 0037906442     PISSN: 09020055     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-302X.2003.180108.x     Document Type: Article

References (22)

1. Al-Robaiy S, Rupf S, Eschrich K. Rapid competitive PCR on the LightCycler using melting curve analysis for DNA quantification. Biotechniques 2001: 6: 1382-1389.
2. Gold OG, Jordan HV, van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol 1973: 18: 1356-1364.
3. Gutgemann A, Golob M, Muller S, Buettner R, Bosserhoff AK. Isolation of invasion-associated cDNAs in melanoma. Arch Dermatol Res 2001: 293: 283-290.
4. Hein I, Lehner A, Rieck P, Klein K, Brandl E, Wagner M. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Appl Environ Microbiol 2001: 67: 3122-3126.
5. Kneist S, Heinrich-Weltzien R, Tietze W, Stößer L. Die Mikrobielle Mundhöhlenbesiedlung Als Grundvoraussetzung Des Kariesrisikos - Eine Übersicht der Befunde der Kinder Aus der Erfurter Studie [The Microbial Colonization of the Oral Cavity as the Basic Condition of Caries Risk - an Overview of the Findings in Children of the Erfurt Caries Risk Study]. In: Stößer L, ed. Kariesdynamik und Kariesrisiko. Berlin: Quintessenz, 1998: 201-213.
6. Kneist S, Heinrich-Weltzien R, Stößer. L. A comparison between commercial kits and conventional methods for the enumeration of mutans streptococci. Caries Res 1996: 30: 286.
7. Lehmann U, Bock O, Glockner S, Kreipe. H. Quantitative molecular analysis of laser-microdissected paraffin-embedded human tissues. Pathobiology 2000: 68: 202-208.
8. Lyons SR, Griffen AL, Leys EJ. Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria. J Clin Microbiol 2000: 38: 2362-2365.
9. Macrina FL, Dertzbaugh MT, Halula MC, Krah ER, Jones KR. Genetic approaches to the study of oral microflora: a review. Crit Rev Oral Biol Med 1990: 3: 207-227.
10. Morrison TB, Weis JJ, Wittwer CT. Quantification of low copy transcripts by continuous SYBR-Green I monitoring during amplification. Biotechniques 1998: 24: 954-962.
11. Najioullah F, Thouvenot D, Lina B. Development of a real-time PCR procedure including an internal control for the measurement of HCMV viral load. J Virol Methods 2001: 92: 55-64.
12. Oho T, Yamashita Y, Shimazaki Y, Kushiyama M, Koga T. Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction. Oral Microbiol Immunol 2000: 15: 258-262.
13. Paster BJ, Boches SK, Galvin JL et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001: 183: 3770-3783.
14. Ririe KM, Rasmussen RP, Wittwer T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997: 245: 154-160.
15. Rocas IN, Siqueira JF Jr, Santos KR, Coelho AM. 'Red complex' (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001: 91: 468-471.
16. Rupf S, Merte K, Eschrich K, Stößer L, Kneist S. The peroxidase reaction as a parameter for discrimination of Streptococcus mutans and Streptococcus sobrinus. Caries Res 2001: 35: 258-264.
17. Rupf S, Merte K, Eschrich K. Quantification of bacteria in oral samples by competitive polymerase chain reaction. J Dent Res 1999: 78: 850-856.
18. Rupf S, Merte K, Eschrich K. Quantitative determination of Streptococcus mutans by using competitive polymerase chain reaction. Eur J Oral Sci 1999: 107: 75-81.
19. Socransky SS, Smith C, Martin L, Paster BJ, Dewhirst FE, Levin AE. 'Checkerboard' DNA-DNA hybridization. Biotechniques 1994: 4: 788-792.
20. Stößer L, ed. Kariesdynamik und Kariesrisiko. Berlin: Quintessenz, 1998.
21. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 1998: 4: 195-216.
22. Yuan K, Hsu PC, Tseng CC, Kiang D, Wang JR. Detection rate of Actinobacillus actinomycetemcomitans on the permanent 1st molars of primary school children in Taiwan by polymerase chain reaction. J Clin Periodontol 2001: 28: 348-352.