Quantitative determination of Streptococcus mutans by using competitive polymerase chain reaction

European Journal of Oral Sciences

Volumn 107, Issue 2, 1999, Pages 75-81

  Rupf, Stefan ^a   Kneist, Susanne ^b   Merte, Knut ^a   Eschrich, Klaus ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

Author keywords

Bacterial* detection; Competitive PCR; Homologous competitor; S. mutans

Indexed keywords

BACTERIAL DNA; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS;

ADULT; ARTICLE; BACTERIAL COUNT; CHILD; COMPARATIVE STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SALIVA; STREPTOCOCCUS MUTANS;

ADULT; CHILD; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; HUMANS; INDICATORS AND REAGENTS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SALIVA; STREPTOCOCCUS MUTANS;

EID: 0033110778     PISSN: 09098836     EISSN: 16000722     Source Type: Journal    
DOI: 10.1046/j.0909-8836.1999.eos107201.x     Document Type: Article

References (28)

1. ORLAND FJ, BLAYNEA JR, HARRISON RW, RAYNIERS JA, TREXLER PC, WAGNER M, GORDON HA, LUCKEY TD. Use of germfree animal technique in the study of experimental dental caries. I. Basic observation on rats were free of all microorganisms. J Dent Res 1954; 33: 141-147.
2. KEYES PH. Research in dental caries. J Am Dent Assoc 1968; 76: 1357-1373.
3. LOESCHE WJ, ROWAN J, STRAFFON ICH, LOOS PJ. Association of S. mutans with human dental decay. Infect Immun 1975; 11: 1252-1260.
4. LOESCHE W. Role of S. mutans in human dental decay. Microbiol Rev 1986; 50: 353-380.
5. KÖNIG KG. Mikroorganismen als Ursache von Karies und Parodontopathien. In: KÖNIG KG, ed. Karies und Parodontopathien. Stuttgart: Thieme Verlag, 1987; 112-220.
6. VAN HOUTE J. Microbiological predictors of caries risk. Adv Dent Res 1993; 7: 87-96.
7. STÖSSER L, KATZMANN M, GROSSER W. The aciduric properties of S. mutans. Zahn Mund Kieferhk Zentralbl 1989; 77: 576-577.
8. KRASSE B. Biological factors as indicators of future caries. Int Dent J 1988; 38: 219-225.
9. ZICKERT I, EMILSON CG, KRASSE B. Effect of caries preventive measures in children highly infected with the bacterium S. mutans. Arch Oral Biol 1982; 27: 861-868.
10. BRATTHALL D. Dental caries: intervented - interrupted - interpreted. Concluding remarks and cariography. Eur J Oral Sci 1996; 104: 486-491.
11. GOLD OG, JORDAN HV, VAN HOUTE J. A selective medium for S. mutans. Arch Oral Biol 1973; 18: 1356-1364.
12. CLARK JK. On the bacterial factor in the etiology of dental caries. Br J Exp Pathol 1924; 5: 141-147.
13. JENSEN B, BRATTHALL D. A new method for the estimation of mutans streptococci in human saliva. J Dent Res 1989; 68: 468-471.
14. WATANABE K, FROMMEL TO. Detection of Porphyromonas gingivalis in oral plaque samples by use of the polymerase chain reaction. J Dent Res 1993; 72: 1040-1044.
15. LISTGARTEN M, WONG MY, LAI CH. Detection of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Bacteroides forsythus in an A. actinomycetemcomitans-positive patient population. J Periodontol 1995; 66: 158-164.
16. WATANABE K, FROMMEL TO. Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola detection in oral plaque samples using the polymerase chain reaction. J Clin Periodontol 1996; 23: 212-219.
17. FURCHT C, ESCHRICH K, MERTE K. Detection of Eikenella corrodens and Actinobacillus actinomycetemcomitans by use of the polymerase chain reaction (PCR) in vitro and in subgingival plaque. J Clin Periodontol 1996; 23: 891-897.
18. ONO T, HIROTA K, NEMOTO K, FERNANDEZ EJ, OTA F, FUKUI K. Detection of S. mutans by PCR amplification of spaP gene. J Med Microbiol 1994; 41: 231-235.
19. IGARASHI T, YAMAMOTO A, GOTO N. Rapid identification of mutans streptococcal species. Microbiol Immunol 1996; 40: 867-871.
20. SLOTS J, ASHIMOTO A, FLYNN MJ, LI G, CHEN C. Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin Inf Dis 1995; 20: 304-307.
21. LARRICK JW. The PCR Technique: Quantitative PCR. Palo Alto, CA: Clontech Laboratories, 1997; 1-325.
22. DE KANT E, ROCHLITZ CF, HERRMANN R. Gene expression analysis by a competitive and differential PCR with antisense competitors. Biotechniques 1994; 19: 934-942.
23. KÖHLER T, LASSNER D, ROST AK, LEIBLEIN S, REMKE H. Quantitation of mRNA by Polymerase Chain Reaction - Nonradioactive Methods. Berlin, Heidelberg: Springer Verlag, 1995; 1-165.
24. SERTH J, PANITZ F, HERRMANN H, ALVES J. Single-tube nested competitive PCR with homologous competitor for quantification of DNA target sequences: theoretical description of heteroduplex formation, evaluation of sensitivity, precision and linearrange of the method. Nucleic Acids Res 1998; 26: 4401-4408.
25. CORPET F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 1988; 16: 10881-10890.
26. KING TC, SCHLESSINGER D. Processing of RNA transcripts. In: NEIDHARD FC, INGRAHAM JL, BROOKS LOW K, MAGASANIK B, SCHAECHTER M, UMBARGER HE, eds. Escherichia coli and Salmonella typhimurium. Cellular and molecular biology. Vol. 1. Washington, D.C.: American Society for Microbiology, 1987; 703-718.
27. GOLD OG, JORDAN HV, VAN HOUTE J. A selective medium for Streptococcus mutans. Arch Oral Biol 1973; 18: 1357-1364.
28. Ethical guidelines of the Fédération Dentaire Internationale with respect to experiments with human beings in clinical investigation. Resolution of the F.D.I, council no. 10 on the 78th Annual World Congress of Dentists. Singapore, 1990.