Metabolic flux analysis of hepatocyte function in hormone- and amino acid-supplemented plasma

Metabolic Engineering

Volumn 5, Issue 1, 2003, Pages 1-15

  Chan, Christina ^a,b   Berthiaume, François ^a   Lee, Kyongbum ^a   Yarmush, Martin L ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b Michigan State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0037837641     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1096-7176(02)00011-3     Document Type: Article

References (40)

1. Agius, L., Alberti, K.G., 1985. Regulation of flux through pyruvate dehydrogenase and pyruvate carboxylase in rat hepatocytes. Effects of fatty acids and glucagon. Eur. J. Biochem. 152, 699-707.
2. Arai, K., et al., 2001. Intrahepatic amino acid and glucose metabolism in a D-Galactosamine-induced rat liver failure model. Hepatology 34, 360-371.
3. Balis, U.J., et al., 1999. Oxygen consumption characteristics of porcine hepatocytes. Metabol. Eng. 1, 49.
4. Berry, M.N., et al., 1988. Evidence that stimulation of gluconeogenesis by fatty acid is mediated through thermodynamic mechanisms. FEBS Lett. 231, 19-24.
5. Brosnan, J.T., Williamson, D.H., 1979. Mechanisms for the formation of alanine and aspartate on rat liver in vivo after administration of ammonium chloride. Biochem J. 138, 453-462.
6. Chan, C., et al., 2002. Metabolic pre-conditioning of cultured cells in physiological levels of insulin: generating resistance to the lipid accumulating effects of plasma in hepatocytes. Biotechnol. Bioeng. 78, 753-760.
7. Chan, C., et al., 2003. Metabolic flux analysis of cultured hepatocytes exposed to plasma. Biotechnol. Bioeng. 81, 33-49.
8. 2-Capture Assay for Evaluation of Substrate Oxidation Rates of Cells in Culture. BioTechniques 24, 803.
9. Dunn, J.C., et al., 1991. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol. Prog. 7, 237-245.
10. Exton, J.H., et al., 1976. Carbohydrate metabolism in perfused livers of adrenalectomized and steroid-replaced rats. Am. J. Physiol. 230, 163-170.
11. Fleig, W.E., et al., 1984. Hormonal regulation of key gluconeogenic enzymes and glucose release in cultured hepatocytes: effects of dexamethasone and gastrointestinal hormones on glucagon action. Biochem. Biophys. 229, 368-378.
12. Foy, B.D., et al., 1994. A device to measure the oxygen uptake rate of attached cells: importance in bioartificial organ design. Cell Transplantation 3, 515.
13. Goldstein, R.E., et al., 1993. Effects of chronic elevation in plasma cortisol on hepatic carbohydrate metabolism. Am. J. Physiol. 264, E119-E127.
14. Hoofnagle, J.H., et al., 1995. Fulminant hepatic failure: summary of a workshop. Hepatology 21, 240.
15. Lee, K., et al., 2000. Metabolic flux analysis of post-burn hepatic hypermetabolism. Metabol. Eng. 2, 312-327.
16. Matthew, H.W.T., et al., 1993. Performance of plasma-perfused, microencapsulated hepatocytes: prospects for extracorpeal liver support. J. Pediatr. Surg. 28, 1423-1428.
17. Matthew, H.W.T., et al., 1996. Effects of plasma exposure on cultured hepatocytes: implications for bioartificial liver support. Biotechnol. Bioeng. 51, 100-111.
18. Meyer, R.D., et al., 1983. Glycerol-3-phosphate dehydrogenase is induced by glucocorticoids in hepatocytes and hepatoma cells in vitro. J. Cell. Phys. 114, 203-208.
19. Nissim, I., et al., 1993. Acid-base regulation of hepatic glutamine metabolism and ureaenesis: study with 15N. J. Am. Soc. Nephrol. 3, 1416-1427.
20. Nissim, I., et al., 1996. Regulation of [15N]urea synthesis from [5-15N]glutamine: role of pH, hormones and pyruvate. J. Biol. Chem. 271, 31234-31242.
21. Nyberg, S.L., Misra, S.P., 1998. Hepatocyte liver-assist systems-a clinical update. Mayo Clin. Proc. 73, 765.
22. OPTN, U. Adapted from UNOS OPTN data as of August 5, 2000.
23. Plas, C., Nunez, J., 1976. Role of cortisol on the glycogenolytic effect of glucagon and on the glycogenic response to insulin in fetal hepatocyte culture. J. Biol. Chem. 251, 1431-1437.
24. Seglen, P.O., 1976. Preparation of isolated rat liver cells. Methods Biol. 13, 29-83.
25. Stefanovich, P., et al., 1996. Extracorporeal plasma perfusion of cultured hepatocytes: effect of intermittent perfusion on hepatocyte function and morphology. J. Surg. Res. 66, 57-63.
26. Tomomura, M., et al., 1996. Long-chain fatty acids suppress the induction of urea cycle enzyme genes by glucocorticoid action. FEBS Lett. 399, 310-312.
27. Tortora, G.J., et al., 2000. Principles of Anatomy and Physiology. Harper & Row, New York.
28. Uchino, J., et al., 1991. A hybrid artificial liver system. Function of cultured monolayer pig hepatocytes in plasma from hepatic failure patients. ASAIO Trans. 37, M337-M338.
29. Wang, N.S., Stephanopoulos, G., 1983. Application of macroscopic balances to the identification of gross measurement errors. Biotechnol. Bioeng. 25, 2177-2208.
30. Washizu, J., et al., 2000aa. Optimization of rat hepatocyte culture in citrated human plasma. J. Surg. Res. 93, 237-246.
31. Washizu, J., et al., 2000bb. Amino acid supplementation improves cell-specific functions of the rat hepatocytes exposed to human plasma. Tissue Eng. 6, 497-504.
32. Watanabe, F.D., et al., 1997. Clinical experience with a bioartificial liver in the treatment of severe liver failure. A phase I clinical trial. Ann. Surg. 225, 484.
33. Yamaguchi, Y., et al., 1997. Effect of burn injury on glucose and nitrogen metabolism in the liver: preliminary studies in a perfused liver system. Surgery 121, 295.
34. Yarmush, M.L., et al., 1992. Assessment of artificial liver support technology. Cell Transplant. 1, 323.
35. Zupke, C., et al., 1995. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl. Microbiol. Biotechnol. 44, 27-36.
36. Zupke, C.A., et al., 1998. Metabolic effects of stress mediators on cultured hepatocytes. Biotechnol. Bioeng. 58, 222-230.
37. Nawa, K., et al.,1986. Glucocorticoid-dependent expression of the albumin gene in adult rat hepatocytes. J. Biol Chem. 261, 16883-16888.
38. Straus, D.S., et al., 1994. Protein restriction specifically decreases the abundance of serum albumin and transthyretin nuclear transcripts in rat liver. J. Nutr. 124, 1041-1051.
39. Wang, C.N., et al., 1995. Effects of dexamethasone on the synthesis, degradation, and secretion of apolipoprotein b in cultured rat hepatocytes. Arterioscler. Thromb. Vasc. Biol. 9, 1481-1491.
40. Yahagi, N., et al., 1999. A crucial role of sterol regulatory element-binding protein-1 in the regulation of lipogenic gene expression by polyunsaturated fatty acids. J. Biol. Chem. 274, 35840-35844.