PCR methodology as a valuable tool for identification of endodontic pathogens

Journal of Dentistry

Volumn 31, Issue 5, 2003, Pages 333-339

  Siqueira Jr , José F ^a,b   Rôças, Isabela N ^a,b  

^a ESTÁCIO DE SÁ UNIVERSITY   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Bacterial identification; Endodontic infection; Polymerase chain reaction

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTEROIDES FORSYTHUS; DIAGNOSTIC ACCURACY; DNA SEQUENCE; ENDODONTICS; EUBACTERIUM; INTERMETHOD COMPARISON; MEDICAL LITERATURE; MOLECULAR CLONING; PATHOGENESIS; POLYMERASE CHAIN REACTION; PORPHYROMONAS ENDODONTALIS; PORPHYROMONAS GINGIVALIS; PREVOTELLA; TOOTH PLAQUE; TOOTH ROOT CANAL; TREPONEMA DENTICOLA;

EID: 0037812020     PISSN: 03005712     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-5712(03)00051-4     Document Type: Article

References (44)

1. Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews. 59:1995;143-169.
2. Relman D.A. The identification of uncultured microbial pathogens. Journal of Infectious Diseases. 168:1993;1-8.
3. Relman D.A. The search for unrecognized pathogens. Science. 284:1999;1308-1310.
4. Wade W. Unculturable bacteria - the uncharacterized organisms that cause oral infections. Journal of the Royal Society of Medicine. 95:2002;81-83.
5. Mombelli A., Minder C.E., Gusberti F.A., Lang N.P. Reproducibility of microscopic and cultural data in repeated subgingival plaque samples. Journal of Clinical Periodontology. 16:1989;434-442.
6. Loesche W.J., Lopatin D.E., Stoll J., van Poperin N., Hujoel P.P. Comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard? Journal of Clinical Microbiology. 30:1992;418-426.
7. Ashimoto A., Chen C., Bakker I., Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiology and Immunology. 11:1996;266-273.
8. Riggio M.P., Macfarlane T.W., Mackenzie D., Lennon A., Smith A.J., Kinane D. Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples. Journal of Periodontal Research. 31:1996;496-501.
9. Sakamoto M., Takeuchi Y., Umeda M., Ishikawa I., Benno Y. Rapid detection and quantification of five periodontopathic bacteria by real-time PCR. Microbiology and Immunology. 45:2001;39-44.
10. Greisen K., Loeffelholz M., Purohit A., Leong D. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. Journal of Clinical Microbiology. 32:1994;335-351.
11. Hofstad T. Utility of newer techniques for classification and identification of pathogenic anaerobic bacteria. Clinical Infectious Diseases. 18:(Suppl. 4):1994;S250-S252.
12. Wang R.-F., Cao W.-W., Cerniglia C.E. PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Applied and Environmental Microbiology. 62:1996;1242-1247.
13. Jordan J.A., Durso M.B. Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia. Journal of Clinical Microbiology. 38:2000;2574-2578.
14. Kong F., Gordon S., Gilbert G.L. Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens. Journal of Clinical Microbiology. 38:2000;4256-4259.
15. Whelen A.C., Persing D.H. The role of nucleic acid amplification and detection in the clinical microbiology laboratory. Annual Reviews in Microbiology. 50:1996;349-373.
16. Pitt T.L., Saunders N.A. Molecular bacteriology: a diagnostic tool for the millennium. Journal of Clinical Pathology. 53:2000;71-75.
17. Mullis K. The unusual origin of the polymerase chain reaction. Scientific American. 262:1990;36-43.
18. Mullis K.B., Ferré F., Gibbs R.A. The polymerase chain reaction. 1994;Birkhäuser, Boston.
19. McPherson M.J., Moller S.G. PCR. 2000;BIOS Scientific Publishers Ltd, Oxford, UK.
20. Eisenstein B.I. The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis. New England Journal of Medicine. 322:1990;178-183.
21. Zambon J.J., Haraszthy V.I. The laboratory diagnosis of periodontal infections. Periodontology 2000. 7:1995;69-82.
22. Siqueira J.F. Jr, Rôças I.N., Souto R., Uzeda M., Colombo A.P. Checkerboard DNA-DNA hybridization analysis of endodontic infections. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics. 89:2000;744-748.
23. Rôças I.N., Siqueira J.F. Jr, Andrade A.F.B., Uzeda M. Oral treponemes in primary root canal infections as detected by nested PCR. International Endodontic Journal. 36:2003;20-26.
24. Belgrader P., Benett W., Hadley D., Richards J., Stratton P., Mariella R. Jr, Milanovich F. PCR detection of bacteria in seven minutes. Science. 284:1999;449-450.
25. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Research. 6:1996;986-994.
26. Woese C.R. Bacterial evolution. Microbiological Reviews. 51:1987;221-271.
27. Tanner A., Maiden M.E.J., Paster B.J., Dewhirst F.E. The impact of 16S ribosomal RNA-based phylogeny on the taxonomy of oral bacteria. Periodontology 2000. 5:1994;26-51.
28. Siqueira J.F. Jr, Rôças I.N., Favieri A., Santos K.R.N. Detection of Treponema denticola in endodontic infections by 16S rRNA gene directed Polymerase Chain Reaction. Oral Microbiology and Immunology. 15:2000;335-337.
29. Siqueira J.F. Jr, Rôças I.N., Oliveira J.C.M., Santos K.R.N. Detection of putative oral pathogens in acute periradicular abscesses by 16S rDNA directed PCR. Journal of Endodontics. 27:2001;164-167.
30. Siqueira J.F. Jr, Rôças I.N., Favieri A., Oliveira J.C.M., Santos K.R.N. Polymerase chain reaction detection of Treponema denticola in endodontic infections within root canals. International Endodontic Journal. 34:2001;280-284.
31. Conrads G., Gharbia S.E., Gulabivala K., Lampert F., Shah H.N. The use of a 16S rDNA PCR for the detection of endodontopathogenic bacteria. Journal of Endodontics. 23:1997;433-438.
32. Gonçalves R.B., Mouton C. Molecular detection of Bacteroides forsythus in infected root canals. Journal of Endodontics. 25:1999;336-340.
33. Rôças I.N., Siqueira J.F. Jr, Santos K.R., Coelho A.M. Red complex (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics. 91:2001;468-471.
34. Xia T., Baumgartner J.C., David L.L. Isolation and identification of Prevotella tannerae from endodontic infections. Oral Microbiology and Immunology. 15:2000;273-275.
35. Sundqvist G., Johansson E., Sjögren U. Prevalence of black-pigmented Bacteroides species in root canal infections. Journal of Endodontics. 15:1989;13-19.
36. Oliveira J.C.M., Siqueira J.F. Jr, Alves G.B., Hirata R. Jr, Andrade A.F.B. Detection of Porphyromonas endodontalis in infected root canals by 16s rRNA gene-directed polymerase chain reaction. Journal of Endodontics. 26:2000;729-732.
37. Siqueira J.F., Rôças I.N., Oliveira J.C.M., Santos K.R.N. Molecular detection of black-pigmented bacteria in infections of endodontic origin. Journal of Endodontics. 27:2001;563-566.
38. Socransky S.S., Haffajee A.D., Cugini M.A., Smith C., Kent R.L. Jr. Microbial complexes in subgingival plaque. Journal of Clinical Periodontology. 25:1998;134-144.
39. Hashimura T., Sato M., Hoshino E. Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method. International Endodontic Journal. 34:2001;463-470.
40. Fouad A.F., Barry J., Caimano M., Clawson M., Zhu Q., Carver R., Hazlett K., Radolf J.D. PCR-based identification of bacteria associated with endodontic infections. Journal of Clinical Microbiology. 40:2002;3223-3231.
41. Rôças I.N., Siqueira J.F. Jr. Identification of Dialister pneumosintes in acute periradicular abscesses of humans by nested PCR. Anaerobe. 8:2002;75-78.
42. Siqueira J.F. Jr, Rôças I.N. Dialister pneumosintes can be a suspected endodontic pathogen. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics. 94:2002;494-498.
43. Rolph H.J., Lennon A., Riggio M.P., Saunders W.P., MacKenzie D., Coldero L., Bagg J. Molecular identification of microorganisms from endodontic infections. Journal of Clinical Microbiology. 39:2001;3282-3289.
44. Munson M.A., Pitt-Ford T., Chong B., Weightman A., Wade W.G. Molecular and cultural analysis of the microflora associated with endodontic infections. Journal of Dental Research. 81:2002;761-766.