Rapid detection and quantification of five periodontopathic bacteria by real-time PCR

Microbiology and Immunology

Volumn 45, Issue 1, 2001, Pages 39-44

  Sakamoto, Mitsuo ^a   Takeuchi, Yasuo ^b   Umeda, Makoto ^b   Ishikawa, Isao ^b   Benno, Yoshimi ^a  

^a RIKEN BIORESOURCE CENTER   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Periodontitis; Periodontopathic bacteria; Quantitative PCR

Indexed keywords

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ANALYTIC METHOD; ARTICLE; BACTERIAL COUNT; BACTERIUM CULTURE; BACTERIUM DETECTION; DISEASE ASSOCIATION; INTERMETHOD COMPARISON; NONHUMAN; PERIODONTAL DISEASE; POLYMERASE CHAIN REACTION; PORPHYROMONAS GINGIVALIS; TANNERELLA FORSYTHENSIS; TREPONEMA; TREPONEMA DENTICOLA; TREPONEMA SOCRANSKII;

ACTINOBACILLUS; ADULT; BACTERIOLOGICAL TECHNIQUES; BACTEROIDES; DNA, BACTERIAL; HUMANS; MIDDLE AGED; PERIODONTAL DISEASES; PERIODONTIUM; POLYMERASE CHAIN REACTION; PORPHYROMONAS GINGIVALIS; SALIVA; SENSITIVITY AND SPECIFICITY; TREPONEMA;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; BACTERIA (MICROORGANISMS); BACTEROIDES; NEGIBACTERIA; PORPHYROMONAS GINGIVALIS; TANNERELLA FORSYTHENSIS; TREPONEMA; TREPONEMA DENTICOLA; TREPONEMA SOCRANSKII;

EID: 0035128715     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2001.tb01272.x     Document Type: Article

References (10)

1. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions
2. The measurement of interrater agreement
3. Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria
4. Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method
5. Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR
6. Detection of Treponema socranskii associated with human periodontitis by PCR
7. Quantitation of Bacteroides forsythus in subgingival plaque. Comparison of immunoassay and quantitative polymerase chain reaction
8. Microbial flora in the acute phase of periodontitis and the effect of local administration of minocycline
9. The utility of whole saliva to detect the oral presence of periodontopathic bacteria
10. Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR