A molecular switch between alternative conformational states in the complex of Ran and importin β1

Nature Structural Biology

Volumn 10, Issue 7, 2003, Pages 553-557

  Nevo, Reinat ^a   Stroh, Cordula ^b   Kienberger, Ferry ^b   Kaftan, David ^a   Brumfeld, Vlad ^a   Elbaum, Michael ^a   Reich, Ziv ^a   Hinterdorfer, Peter ^b  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS RECEPTOR; IMPORTIN BETA1; KARYOPHERIN BETA; MUTANT PROTEIN; RAN PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC FORCE MICROSCOPY; CELL NUCLEUS; COMPLEX FORMATION; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; INTRACELLULAR TRANSPORT; MACROMOLECULE; NONHUMAN; NUCLEAR PORE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; SPECTROSCOPY;

BETA KARYOPHERINS; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; MICROSCOPY, ATOMIC FORCE; PROTEIN BINDING; PROTEIN CONFORMATION; RAN GTP-BINDING PROTEIN;

ESCHERICHIA COLI;

EID: 0037743522     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/nsb940     Document Type: Article

References (37)

1. Smith, A.E., Slepchenko, B.M., Schaff, J.C., Loew, L.M. & Macara, I.G. Systems analysis of Ran transport. Science 295, 488-91 (2002).
2. Kuersten, S., Ohno, M. & Mattaj, I.W. Nucleocytoplasmic transport: Ran, beta and beyond. Trends Cell. Biol. 11, 497-503 (2001).
3. Gorlich, D. & Kutay, U. Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell. Dev. Biol. 15, 607-660 (1999).
4. Mattaj, I.W. & Englmeier, L. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67, 265-306 (1998).
5. Kumar, S., Ma, B., Tsai, C.J., Sinha, N. & Nussinov, R. Folding and binding cascades: dynamic landscapes and population shifts. Protein Sci. 9, 10-19 (2000).
6. Tang, K.E.S. & Dill, K.A. How experiments see fluctuations of native proteins: perspective from an exact model. Int. J. Quantum Chem. 75, 147-164 (1999).
7. Binnig, G., Quate, C.F. & Gerber, C. Atomic force microscope. Phys. Rev. Lett. 56, 930-933 (1986).
8. Florin, E.L., Moy, V.T. & Gaub, H.E. Adhesion forces between individual ligand-receptor pairs. Science 264, 415-417 (1994).
9. Lee, G.U., Kidwell, D.A. & Colton, R.J. Sensing discrete streptavidin biotin interactions with atomic-force microscopy. Langmuir 10, 354-357 (1994).
10. Hinterdorfer, P., Baumgartner, W., Gruber, H.J., Schilcher, K. & Schindler, H. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. Proc. Natl. Acad. Sci. USA 93, 3477-3481 (1996).
11. Evans, E. Probing the relation between force - lifetime - and chemistry in single molecular bonds. Annu. Rev. Biophys. Biomol. Struct. 30, 105-128 (2001).
12. Evans, E. Energy landscapes of biomolecular adhesion and receptor anchoring at interfaces explored with dynamic force spectroscopy. Faraday Discuss. 111, 1-16 (1998).
13. Evans, E. & Ritchie, K. Dynamic strength of molecular adhesion bonds. Biophys. J. 72, 1541-1555 (1997).
14. Merkel, R., Nassoy, P., Leung, A., Ritchie, K. & Evans, E. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy. Nature 397, 50-53 (1999).
15. Strunz, T., Oroszlan, K., Schafer, R. & Guntherodt, H.J. Dynamic force spectroscopy of single DNA molecules. Proc. Natl. Acad. Sci. USA 96, 11277-11282. (1999).
16. Baumgartner, W. et al. Cadherin interaction probed by atomic force microscopy. Proc. Natl. Acad. Sci. USA 97, 4005-4010 (2000).
17. Scheffzek, K., Klebe, C., Fritz-Wolf, K., Kabsch, W. & Wittinghofer, A. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form. Nature 374, 378-381 (1995).
18. Stewart, M., Kent, H.M. & McCoy, A.J. Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran. J. Mol. Biol. 277, 635-646 (1998).
19. Vetter, I.R., Arndt, A., Kutay, U., Gorlich, D. & Wittinghofer, A. Structural view of the Ran-Importin β interaction at 2.3 Å resolution. Cell 97, 635-646 (1999).
20. Vetter, I.R., Nowak, C., Nishimoto, T., Kuhlmann, J. & Wittinghofer, A. Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport. Nature 398, 39-46 (1999).
21. Villa Braslavsky, C.I., Nowak, C., Gorlich, D., Wittinghofer, A. & Kuhlmann, J. Different structural and kinetic requirements for the interaction of Ran with the Ran-binding domains from RanBP2 and importin-β. Biochemistry 39, 11629-11639 (2000).
22. Bartolo, D., Derenyi, I. & Ajdari, A. Dynamic response of adhesion complexes: beyond the single-path picture. Phys. Rev. E65, 051910 (2002).
23. Klebe, C., Bischoff, F.R., Ponstingl, H. & Wittinghofer, A. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1. Biochemistry 34, 639-647 (1995).
24. Stewart, M., Kent, H.M. & McCoy, A.J. The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2). J. Mol. Biol. 284, 1517-1527 (1998).
25. Volkman, B.F., Lipson, D., Wemmer, D.E. & Kern, D. Two-state allosteric behavior in a single-domain signaling protein. Science 291, 2429-2433 (2001).
26. Malmendal, A., Evenas, J., Forsen, S. & Akke, M. Structural dynamics in the C-terminal domain of calmodulin at low calcium levels. J. Mol. Biol. 293, 883-899 (1999).
27. Cingolani, G., Petosa, C., Weis, K. & Muller, C.W. Structure of importin-β bound to the IBB domain of importin-β. Nature 399, 221-229 (1999).
28. Lee, S.J. et al. The adoption of a twisted structure of importin-β is essential for the protein-protein interaction required for nuclear transport. J. Mol. Biol. 302, 251-264 (2000).
29. Chook, Y.M. & Blobel, G. Structure of the nuclear transport complex karyopherin-β2-Ran x GppNHp. Nature 399, 230-237 (1999).
30. Chook, Y.M. & Blobel, G. Karyopherins and nuclear import. Curr. Opin. Struct. Biol. 11, 703-715 (2001).
31. Geyer, M. et al. Conformational states of the nuclear GTP-binding protein Ran and its complexes with the exchange factor RCC1 and the effector protein RanBP1. Biochemistry 38, 11250-11260 (1999).
32. Geyer, M. et al. Conformational transitions in p21ras and in its complexes with the effector protein Raf-RBD and the GTPase activating protein GAP. Biochemistry 35, 10308-10320 (1996).
33. Chi, N.C., Adam, E.J., Visser, G.D. & Adam, S.A. RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import. J. Cell Biol. 135, 559-569 (1996).
34. Hinterdorfer, P. et al. Surface attachment of ligands and receptors for molecular recognition force microscopy. Colloids Surf. B 23, 115-123 (2002).
35. Raab, A. et al. Antibody recognition imaging by force microscopy. Nat. Biotechnol. 17, 901-905 (1999).
36. Hutter, J.L. & Bechhoefer, J. Calibration of atomic-force microscope tips. Rev. Sci. Instrum. 64, 1868-1873 (1993).
37. Baumgartner, W., Hinterdorfer, P. & Schindler, H. Data analysis of interaction forces measured with the atomic force microscope. Ultramicroscopy 82, 85-95 (2000).