Two-step purification of His-tagged Nef protein in native condition using heparin and immobilized metal ion affinity chromatographies

Journal of Virological Methods

Volumn 111, Issue 1, 2003, Pages 69-73

  Finzi, Andrés ^a   Cloutier, Jonathan ^a   Cohen, Éric A ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

GCHI; Heparin affinity chromatography; His Nef; IMAC; Purification; SlyD

Indexed keywords

BACTERIAL PROTEIN; HEPARIN; NEF PROTEIN;

ARTICLE; BACTERIUM; CONTAMINATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

BACTERIA (MICROORGANISMS); HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0037735215     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(03)00154-X     Document Type: Article

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