Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus

Proceedings of the National Academy of Sciences of the United States of America

Volumn 88, Issue 20, 1991, Pages 8972-8976

  Janknecht, Ralf ^a   De Martynoff, Guy ^b   Lou, Jueren ^b,c   Hipskind, Robert A ^a   Nordheim, Alfred ^a   Stunnenberg, Hendrik G ^b  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c BRISTOL MYERS SQUIBB   (United States)

Author keywords

In vitro transcription; Ni2+ chelate affinity chromatography; Serum response factor

Indexed keywords

VACCINIA; VACCINIA VIRUS;

VIRUS VECTOR;

ARTICLE; GENE EXPRESSION; GENETIC TRANSCRIPTION; HELA CELL; HUMAN; HUMAN CELL; OSTEOSARCOMA; PRIORITY JOURNAL; PROTEIN PROCESSING; PROTEIN PURIFICATION; VACCINIA VIRUS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA-BINDING PROTEINS; GENETIC VECTORS; HELA CELLS; HISTIDINE; HUMAN; INDICATORS AND REAGENTS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; OSTEOSARCOMA; PLASMIDS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SERUM RESPONSE FACTOR; SUPPORT, NON-U.S. GOV'T; TATA BOX; TRANSCRIPTION FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED; VACCINIA VIRUS;

EID: 0025946803     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.88.20.8972     Document Type: Article

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