Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 4982, Issue , 2003, Pages 8-20

  Kim, Jung Kyung ^a   Bang, Hyunwoo ^b   Chung, Seok ^a   Chin Yu, Alexey Dan ^a   Chung, Chanil ^a   Jo, Han Sang ^a   Yoo, Jung Yul ^b   Chang, Jun Keun ^a,b  

^a Digital Bio Technology Co   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Bio MEMS; Flow cytometry; Hydrodynamic focusing; Lab on a chip; Microchip; Microfabrication; Microfluidics; Microsystem; Single cell manipulation; Soft lithography

Indexed keywords

FLUIDICS; FLUORESCENCE; LASER BEAMS; MICROELECTROMECHANICAL DEVICES;

PLASTIC MICROFLUIDIC CHIPS;

MICROPROCESSOR CHIPS;

EID: 0037721716     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.472741     Document Type: Conference Paper

References (24)

1. L. Shapiro and T. Harris, "Finding function through structural genomics," Curr. Opin. Biotechnol. 11, pp. 31-35, 2000.
2. P. S. Lee and K. H. Lee, "Genomic analysis," Curr. Opin. Biotechnol. 11, pp. 171-175, 2000.
3. G. H. W. Sanders and A. Manz, "Chip-based microsystems for genomic and proteomic analysis," Trends Anal. Chem. 19, pp. 364-378, 2000.
4. D. Vetter, "Miniaturization for drug discovery applications," Drug Discovery Today 3, pp. 404-408, 1998.
5. S. A. Sundberg, "High-throughput and ultra-high-throughput screening: solution- and cell-based approaches," Curr.Opin. Biotechnol. 11, pp. 47-53, 2000.
6. J. P. Nolan, Lauer, S., E. R. Prossnitz, and L. A. Sklar, "Flow cytometry: a versatile tool for all phases of drug discovery," Drug Discovery Today 4, pp. 173-180, 1999.
7. H. M. Shapiro, "Microbial analysis at the single-cell level: tasks and techniques," J. Microbiol. Methods 42, pp. 3-16, 2000.
8. D. Beebe, "Microfabricated fluidic devices for single cell handling and analysis," In Emerging Tools for Single Cell Analysis: Advances in Optical Measurement Technologies ed. G Durack, J Robinson., Wiley, New York, 2000.
9. A. Y. Fu, C. Spence, A. Scherer, F. H. Arnold, and S. R. Quake, "A microfabricated fluorescence-activated cell sorter," Nat. Biotechnol. 17, pp. 1109-1111, 1999.
10. S. Chung, S.-J. Park, J. K. Kim, C. Chung, D. C. Han, and J. K. Chang, "Plastic microchip flow cytometer based on 2 and 3 dimensional hydrodynamic flow focusing," Microsystem Technol., accepted for publication.
11. nd ed., MCGraw Hill, New York, 1991.
12. J. K. Kim, H. Bang, Y. Lee, C. Chung, J. Y. Yoo, S. S. Yang, J. S. Kim, S. Park, and J. K. Chang, "Micro-imaging techniques for evaluation of plastic microfluidic chip," J. Semiconductor Tech. and Sci. 1, pp. 239-247, 2001.
13. L.-Q. Li and L. M. Davis, "Rapid and efficient detection of single chromophore molecules in aqueous solution," Appl. Optics 34, pp. 3208-3217, 1995.
14. J. Enderlein, D. L. Robbins, W. P. Ambrose, P. M. Goodwin, and R. A. Keller, "The statistics of single molecule detection: an overview," Bioimaging 5, pp. 88-98, 1997.
15. D. H. Bunfield and L. M. Davis, "Monte Carlo simulation of a single-molecule detection experiment," Appl. Optics 37, pp. 2315-2326, 1998.
16. H. L. Goldsmith and V. T. Turitto, "Rheological aspects of thrombosis and haemostasis: basic principles and applications," Thromb. Haemost. 55, pp. 415-435, 1986.
17. X. Tong and K. D. Caldwell, "Separation and characterization of red blood cells with different membrane deformability using steric field-flow fractionation," J. Chromatogr. B 674, pp. 39-47, 1995.
18. Y. Zhao and M. K. Sharp, "Finite element analysis of the lift on a slightly deformable and freely rotating and translating cylinder in two-dimensional channel flow," J. Biomech. Eng. 121, pp. 148-152, 1999.
19. P. S. Williams, M. H. Moon, Y. Xu, and J. C. Giddings, "Effect of viscosity on retention time and hydrodynamic lift force in sedimentation/steric field-flow fractionation," Chem. Eng. Sci. 51, pp. 4477-4488, 1996.
20. S. Mortazavi and G. Tryggvason, "A numerical study of the motion of drops in Poiseuille flow. Part 1. Lateral migration of one drop," J. Fluid Mech. 411, pp. 325-350, 2000.
21. D. Joseph and D. Ocando, "Slip velocity and lift," J. Fluid Mech. 454, pp. 263-286, 2002.
22. V. Kachel, E. Kordwig, and G. Glossner, "Uniform lateral orientation, caused by flow forces, of flat particles in flow-through systems," J. Histochem. Cytochem. 25, pp. 774-780, 1977.
23. M. J. Fulwyler, "Hydrodynamic orientation of cells," J. Histochem. Cytochem. 25, pp. 781-783, 1977.
24. R. T. Stovel, R. G. Sweet, and L. A. Herzenberg, "A means for orienting flat cells in flow systems," Biophys. J. 23, pp. 1-5, 1978.