Efficient expression of isotopically labeled peptides for high resolution NMR studies: Application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans

Journal of Biomolecular NMR

Volumn 26, Issue 4, 2003, Pages 317-326

  Osborne, Michael J ^a   Su, Zhengding ^a   Sridaran, Vasanth ^a   Ni, Feng ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Isotope labelling; NMR; Peptide expression

Indexed keywords

CARRIER PROTEIN; CYANOGEN BROMIDE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; METHIONINE; PEPTIDE; PROTEIN CDC42; PROTEIN KINASE; PROTEINASE; RAC PROTEIN;

ARTICLE; CELL INCLUSION; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SOLID; VIRULENCE;

AMINO ACID SEQUENCE; BINDING SITES; CANDIDA ALBICANS; CARBON ISOTOPES; CDC42 GTP-BINDING PROTEIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CONSENSUS SEQUENCE; CYANOGEN BROMIDE; DEUTERIUM; ESCHERICHIA COLI; INCLUSION BODIES; ISOTOPE LABELING; METHIONINE; MOLECULAR SEQUENCE DATA; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDE FRAGMENTS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; RAC GTP-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS;

CANDIDA; CANDIDA ALBICANS; ESCHERICHIA COLI;

EID: 0037710753     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1024043620972     Document Type: Article

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