Expression, purification, and isotope labeling of a gp120 v3 peptide and production of a fab from a hiv-1 neutralizing antibody for nmr studies

Protein Expression and Purification

Volumn 24, Issue 3, 2002, Pages 374-383

  Sharon, Michal ^a   Görlach, Matthias ^b   Levy, Rina ^a   Hayek, Yehezkiel ^a   Anglister, Jacob ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

Antibody; Isotope labeling; NMR; Peptide expression

Indexed keywords

ANIMALIA; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0036451443     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1577     Document Type: Article

References (36)

1. Rusche, J. R., Javaherian, K., McDanal, C., Petro, J., Lynn, D. L., Grimaila, R., Langlois, A., Gallo, R. C., Arthur, L. O., Fischinger, P. J., et al. (1988) Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120. Proc. Natl. Acad. Sci. USA 85, 3198-3202.
2. Palker, T. J., Clark, M. E., Langlois, A. J., Matthews, T. J., Weinhold, K. J., Randall, R. R., Bolognesi, D. P., and Haynes, B. F. (1988) Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides. Proc. Natl. Acad. Sci. USA 85, 1932-1936.
3. Goudsmit, J., Debouck, C., Meloen, R. H., Smit, L., Bakker, M., Asher, D. M., Wolff, A. V., Gibbs, C. J., Jr., and Gajdusek, D. C. (1988) Human immunodeficiency virus type 1 neutralization epitope with conserved architecture elicits early type-specific antibodies in experimentally infected chimpanzees. Proc. Natl. Acad. Sci. USA 85, 4478-4482.
4. LaRosa, G. J., Davide, J. P., Weinhold, K., Waterbury, J. A., Profy, A. T., Lewis, J. A., Langlois, A. J., Dreesman, G. R., Boswell, R. N., Shadduck, P., et al. (1990) Conserved sequence and structural elements in the HIV-1 principal neutralizing determinant. Science 249, 932-935.
5. Wahlberg, J., Albert, J., Lundeberg, J., Von Gegerfelt, A., Broliden, K., Utter, G., Fenyo, E. M., and Uhlen, M. (1991) Analysis of the V3 loop in neutralization-resistant human immunodeficiency virus type 1 variants by direct solid-phase DNA sequencing. AIDS Res. Hum. Retroviruses 7, 983-990.
6. Wu, L., Gerard, N. P., Wyatt, R., Choe, H., Parolin, C., Ruffing, N., Borsetti, A., Cardoso, A. A., Desjardin, E., Newman, W., Gerard, C., and Sodroski, J. (1996) CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5. Nature 384, 179-183.
7. Gorny, M. K., Moore, J. P., Conley, A. J., Karwowska, S., Sodroski, J., Williams, C., Burda, S., Boots, L. J., and Zolla Pazner, S. (1994) Human anti-V2 monoclonal antibody that neutralizes primary but not laboratory isolates of human immunodeficiency virus type 1. J. Virol. 68, 8312-8320.
8. Nyambi, P. N., Gorny, M. K., Bastiani, L., van der Groen, G., Williams, C., and Zolla-Pazner, S. (1998) Mapping of epitopes exposed on intact human immunodeficiency virus type 1 (HIV-1) virions: A new strategy for studying the immunologic relatedness of HIV-1. J. Virol. 72, 9384-9391.
9. Bou Habib, D. C., Roderiquez, G., Oravecz, T., Berman, P. W., Lusso, P., and Norcross, M. A. (1994) Cryptic nature of envelope V3 region epitopes protects primary monocytotropic human immunodeficiency virus type 1 from antibody neutralization. J. Virol. 68, 6006-6013.
10. Matthews, T. J. (1994) Tenth anniversary perspectives on AIDS. AIDS Res. Hum. Retroviruses 10, 631-632.
11. Vancott, T. C., Polonis, V. R., Loomis, L. D., Michael, N. L., Nara, P. L., and Birx, D. L. (1995) Differential role of V3-specific antibodies in neutralization assays involving primary and laboratory-adapted isolates of HIV type 1. AIDS Res. Hum. Retroviruses 11, 1379-1391.
12. VanCott, T. C., Bethke, F. R., Polonis, V. R., Gorny, M. K., Zolla-Pazner, S., Redfield, R. R., and Birx, D. L. (1994) Dissociation rate of antibody-gp120 binding interactions is predictive of V3-mediated neutralization of HIV-1. J. Immunol. 153, 449-459.
13. Neurath, A. R., and Strick, N. (1990) Confronting the hypervaria-bility of an immunodominant epitope eliciting virus neutralizing antibodies from the envelope glycoprotein of the human immunodeficiency virus type 1 (HIV-1). Mol. Immunol. 27, 539-549.
14. Holley, L. H., Goudsmit, J., and Karplus, M. (1991) Prediction of optimal peptide mixtures to induce broadly neutralizing antibodies to human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 88, 6800-6804.
15. Kwong, P. D., Wyatt, R., Robinson, J., Sweet, R. W., Sodroski, J., and Hendrickson, W. A. (1998) Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody. Nature 393, 648-659.
16. Rizzuto, C. D., Wyatt, R., Hernandez-Ramos, N., Sun, Y., Kwong, P. D., Hendrickson, W. A., and Sodroski, J. (1998) A conserved HIV gp120 glycoprotein structure involved in chemokine receptor binding. Science 280, 1949-1953.
17. Ghiara, J. B., Stura, E. A., Stanfield, R. L., Profy, A. T., and Wilson, I. A. (1994) Crystal structure of the principal neutralization site of HIV-1. Science 264, 82-85.
18. Stanfield, R., Cabezas, E., Satterthwait, A., Stura, E., Profy, A., and Wilson, I. (1999) Dual conformations for the HIV-1 gp120 V3 loop in complexes with different neutralizing fabs. Structure Fold. Des. 7, 131-142.
19. Rini, J. M., Stanfield, R. L., Stura, E. A., Salinas, P. A., Profy, A. T., and Wilson, I. A. (1993) Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen. Proc. Natl. Acad. Sci. USA 90, 6325-6329.
20. Zvi, A., Feigelson, D. J., Hayek, Y., and Anglister, J. (1997) Conformation of the principal neutralizing determinant of human immunodeficiency virus type 1 in complex with an anti-gp120 virus neutralizing antibody studied by two-dimensional nuclear magnetic resonance difference spectroscopy. Biochemistry 36, 8619-8627.
21. Tugarinov, V., Zvi, A., Levy, R., and Anglister, J. (1999) A cis proline turn linking two beta-hairpin strands in the solution structure of an antibody-bound HIV-1(IIIB) V3 peptide. Nat. Struct. Biol. 6, 331-335.
22. Weliky, D. P., Bennett, A. E., Zvi, A., Anglister, J., Steinbach, P. J., and Tycko, R. (1999) Solid-state NMR evidence for an antibody-dependent conformation of the V3 loop of HIV-1 gp120. Nat. Struct. Biol. 6, 141-145.
23. Wider, G., and Wuhrich, K. (1999) NMR spectroscopy of large molecules and multimolecular assemblies in solution. Curr. Opin. Struct. Biol. 9, 594-601.
24. Tjandra, N., and Bax, A. (1997) Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium. Science 278, 1111-1114.
25. Gorlach, M., Burd, C. G., and Dreyfuss, G. (1994) The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. J. Biol. Chem. 269, 23074-23078.
26. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60-89.
27. Venters, R. A., Huang, C. C., Farmer, B. T., II, Trolard, R., Spicer, L. D., and Fierke, C. A. (1995) High-level 2H/13C/15N labeling of proteins for NMR studies. J. Biomol. NMR 5, 339-344.
28. Gross, E. (1967) The cyanogen bromide reaction. Methods Enzymol. 11, 238-255.
29. Pervushin, K., Riek, R., Wider, G., and Wuthrich, K. (1997) Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc. Natl. Acad. Sci. USA 94, 12366-12371.
30. Cavanagh, J., and Rance, M. (1990) Sensitivity improvements in isotropic mixing (TOCSY) experiments. J. Magn. Reson. 88, 72.
31. Markley, J. L., Bax, A., Arata, Y., Hilbers, C. W., Kaptein, R., Sykes, B. D., Wright, P. E., and Wuthrich, K. (1998) Recommendations for the presentation of NMR structures of proteins and nucleic acids. J. Mol. Biol. 280, 933-952.
32. Folkers, P. J., Nilges, M., Folmer, R. H., Konings, R. N., and Hilbers, C. W. (1994) The solution structure of the Tyr41His mutant of the single-stranded. J. Mol. Biol. 236, 229-246.
33. Ikura, M., and Bax, I. (1992) Isotope filtered 2D NMR of a protein-peptide complex: Study of a skeletal muscle myosin light chain kinase bound to calmodulin. J. Am. Chem. Soc. 114, 2433-2440.
34. Zvi, A., Kustanovich, I., Feigelson, D., Levy, R., Eisenstein, M., Matsushita, S., Richalet-Secordel, P., Regenmortel, M. H., and Anglister, J. (1995) NMR mapping of the antigenic determinant recognized by an anti-gp120, human immunodeficiency virus neutralizing antibody. Eur. J. Biochem. 229, 178-187.
35. Kwong, P. D., Wyatt, R., Desjardins, E., Robinson, J., Culp, J.S., Hellmig, B. D., Sweet, R. W., Sodroski, J., and Hendrickson, W. A. (1999) Probability analysis of variational crystallization and its application to gp120, the exterior envelope glycoprotein of type 1 human immunodeficiency virus (HIV-1). J. Biol. Chem. 274, 4115-4123.
36. Zvi, A., Hiller, R., and Anglister, J. (1992) Solution conformation of a peptide corresponding to the principal neutralizing determinant of HIV-1IIIB: A two-dimensional NMR study. Biochemistry 31, 6972-6979.