Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids

In Vitro Cellular and Developmental Biology - Animal

Volumn 32, Issue 4, 1996, Pages 197-203

  Peshwa, Madhusudan V ^a   Wu, Florence J ^a   Sharp, Harvey L ^b   Cerra, Frank B ^b   Hu, Wei Shou ^a  

^a University of Minnesota   (United States)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

hepatocyte; multicellular aggregate; spheroid; time lapse microscopy

Indexed keywords

ALBUMIN; LIDOCAINE;

ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL MOTION; CELL ULTRASTRUCTURE; CELL VIABILITY; CONTROLLED STUDY; INTRAHEPATIC BILE DUCT; LIVER CELL; MALE; NONHUMAN; PROTEIN SYNTHESIS; RAT; SPHEROID CELL;

ANIMALIA;

EID: 0029945884     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02722946     Document Type: Article

References (14)

1. Asano, K.; Koide, N.; Tsuji, T. Ultrastructure of multicellular spheroids formed in the primary culture of adult rat hepatocytes. J. Clin. Electron Microsc. 22:243-252; 1989.
2. Barcellos-Hoff, M. H.; Aggeler, J.; Ram, T. G., et al. Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane. Development 105:223-235; 1989.
3. Ben-Ze'ev, A.; Robinson, G. S.; Bucher, N. L., et al. Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. Proc. Natl. Acad. Sci. USA 85:2161-2165; 1988.
4. Halban, P. A.; Powers, S. L.; George, K. L., et al. Spontaneous reassociation of dispersed adult rat pancreatic islet cells into aggregates with three-dimensional architecture typical of native islets. Diabetes 36:783-790; 1987.
5. Koide, N.; Asano, K.; Sakaguchi, K., et al. Electron microscopic observation of vermipodia-like processes of multicellular spheroids formed in the primary culture. J. Clin. Electron Microsc. 22:5-6; 1989.
6. Koide, N.; Sakaguchi, K.; Koide, Y., et al. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp. Cell Res. 186:227-235; 1990.
7. Koide, N.; Shinji, T.; Tanabe, T., et al. Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans. Biochem. Biophys. Res. Commun. 161:385-391; 1989.
8. Nyberg, S. L.; Shatford, R. A.; Peshwa, M. V., et al. Evaluation of hepatocyte function in a bioartificial liver: a potential device for the treatment of liver failure. Biotechnol. Bioeng. 41:194-203; 1993.
9. Nyberg, S. L.; Shirabe, K.; Peshwa, M., et al. Extracorporeal application of a gel-entrapment, bioartificial liver: demonstration of drug metabolism and other biochemical functions. Cell Transplantation 2:441-452; 1993.
10. Peshwa, M. V.; Wu, F. J.; Follstad, B. D., et al. Kinetics of hepatocyte spheroid formation. Biotechnol. Prog. 10:460-466; 1994.
11. Seglen, P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 13:29-38; 1976.
12. Shannon, J. M.; Mason, R. J.; Jennings, S. D. Functional differentiation of alveolar type II epithelial cells in vitro: effects of cell shape, cell-matrix interactions and cell-cell interactions. Biochim. Biophys. Acta 931:143-156; 1987.
13. Shinji, T.; Koide, N.; Tsuji, T. Glycosaminoglycans partially substitute for proteoglycans in spheroid formation of adult rat hepatocytes in primary culture. Cell Struct. Funct. 13:179-188; 1988.
14. Tong, J. Z.; De, L. P.; Furlan, V., et al. Long-term culture of adult rat hepatocyte spheroids. Exp. Cell Res. 200:326-332; 1992.