Reproducible and reliable immunohistochemical demonstration of thymidylate synthase in formalin-fixed, paraffin-embedded sections: Application of antigen retrieval in EDTA solution

Acta Histochemica et Cytochemica

Volumn 36, Issue 2, 2003, Pages 115-118

  Kamoshida, Shingo ^a   Matsuoka, Hiroshi ^a   Matsuyama, Atsuji ^a   Shimomura, Ryoichi ^a   Maruta, Morito ^a   Tsutsumi, Yutaka ^a  

^a FUJITA HEALTH UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Antigen retrieval; EDTA; Immunohistochemistry; Pressure cooking; Thymidylate synthase

Indexed keywords

ANTIGEN; BUFFER; CITRIC ACID; EDETIC ACID; FLUOROURACIL; FORMALDEHYDE; MONOCLONAL ANTIBODY; PARAFFIN; POLYCLONAL ANTIBODY; PROTEINASE K; THYMIDYLATE SYNTHASE; TRYPSIN;

ABSORPTION; ANTIGEN RETRIEVAL; ANTIGENICITY; ARTICLE; CANCER; CANCER CELL; CASE REPORT; CONTROLLED STUDY; COOKING; DOSE RESPONSE; ENZYME LOCALIZATION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; PH; PROGNOSIS; PROTEIN EXPRESSION; RELIABILITY; REPRODUCIBILITY;

EID: 0037657682     PISSN: 00445991     EISSN: None     Source Type: Journal    
DOI: 10.1267/ahc.36.115     Document Type: Article

References (20)

1. Aschele, C., Debemardis, D., Casazza, S., Antonelli, G., Tunesi, G., Baldo, C., Lionetto, R., Maley, F. and Sobrero, A. (1999) Immunohistochemical quantitation of thymidylate synthase expression in colorectal cancer metastases predicts for clinical outcome to fluorouracil-based chemotherapy. J. Clin. Oncol. 17; 1760-1770.
2. Beck, A., Etienne, M. C., Cheradame, S., Fischel, J. L., Formento, P., Renee, N. and Milano, G. (1994) A role for dihydropyrimidine dehydrogenase and thymidylate synthase in tumour sensitivity to fluorouracil. Eur. J. Cancer 30A; 1517-1522.
3. Bussolati, G., Gugliotta, P., Volante, M., Pace, M. and Papotti, M. (1997) Retrieved endogenous biotin: A novel marker and a potential pitfall in diagnostic immunohistochemistry. Histopathology 31; 400-407.
4. Cascinu, S., Aschele, C., Bami, S., Debemardis, D., Baldo, C., Tunesi, G., Catalano, V., Staccioli, M. P., Brenna, A., Muretto, P. and Catalano, G. (1999) Thymidylate synthase protein expression in advanced colon cancer: Correlation with the site of metastasis and the clinical response to leucovorin-modulated bolus 5-fluorouracil. Clin. Cancer Res. 5; 1996-1999.
5. Corsi, D. C., Ciaparrone, M., Zannoni, G., Mancini, M., Cassano, A., Specchia, M., Pozzo, C., Martini, M. and Barone, C. (2002) Predictive value of thymidylate synthase expression in resected metastases of colorectal cancer. Eur. J. Cancer 38; 527-534.
6. Edler, D., Hallström, M., Johnston, P. G., Magnusson, I., Ragnhammar, P. and Blomgren, H. (2000) Thymidylate synthase expression: An independent prognostic factor for local recurrence, distant metastasis, disease-free and overall survival in rectal cancer. Clin. Cancer Res. 6; 1378-1384.
7. Johnston, P. G., Fisher, E. R., Rockette, H. E., Fisher, B., Wolmark, N., Drake, J. C., Chabner, B. A. and Allegra, C. J. (1994) The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer. J. Clin. Oncol. 12; 2640-2647.
8. Johnston, P. G., Lenz, H.-J., Leichman, C. G., Danenberg, K. D., Allegra, C. J., Danenberg, P. V. and Leichman, L. (1995) Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors. Cancer Res. 55; 1407-1412.
9. Kuniyasu, T., Nakamura, T., Tabuchi, Y. and Kuroda, Y. (1998) Immunohistochemical evaluation of thymidylate synthase in gastric carcinoma using a new polyclonal antibody. Cancer 83; 1300-1306.
10. McKay, J. A., Lloret, C., Murray, G. I., Johnston, P. G., Bicknell, R., Ahmed, F. Y., Cassidy, J. and McLeod, H. L. (2000) Application of the enrichment approach to identify putative markers of response to 5-fluorouracil therapy in advanced colorectal carcinomas. Int. J. Oncol. 17; 153-158.
11. Morgan, J. M., Navabi, H., Schmid, K. W. and Jasani, B. (1994) Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J. Pathol. 174; 301-307.
12. Peters, G. J., van der Wilt, C. L. and van Groeningen, C. J. (1994) Predictive value of thymidylate synthase and dihydropyrimidine dehydrogenase. Eur. J. Cancer 30A; 1408-1411.
13. Pileri, S. A., Roncador, G., Ceccarelli, C., Piccioli, M., Briskomatis, A., Sabattini, E., Ascani, S., Santini, D., Piccaluga, P. P., Leone, O., Damiani, S., Ercolessi, C., Sandri, F., Pieri, F., Leoncini, L. and Falini, B. (1997) Antigen retrieval techniques in immunohistochemistry: Comparison of different methods. J. Pathol. 183; 116-123.
14. Spears, C. P., Shahinian, A. H., Moran, R. G., Heidelberger, C. and Corbett, T. H. (1982) In vivo kinetics of thymidylate synthetase inhibition in 5-fluorouracil-sensitive and -resistant murine colon adenocarcinomas. Cancer Res. 42; 450-456.
15. Suda, Y., Kuwashima, Y., Tanaka, Y., Uchida, K. and Akazawa, S. (1999) Immunohistochemical detection of thymidylate synthase in advanced gastric cancer: A prognostic indicator in patients undergoing gastrectomy followed by adjuvant chemotherapy with 5-fluoropyrimidines. Anticancer Res. 19; 805-810.
16. Takenoue, T., Nagawa, H., Matsuda, K., Fujii, S., Nita, M. E., Hatano, K., Kitayama, J., Tsuruo, T. and Muto, T. (2000) Relation between thymidylate synthase expression and survival in colon carcinoma, and determination of appropriate application of 5-fluorouracil by immunohistochemical method. Ann. Surg. Oncol. 7; 193-198.
17. Tomiak, A., Vincent, M., Earle, C. C., Johnston, P. G., Kocha, W., Taylor, M., Maroun, J., Eidus, L., Whinston, F. and Stitt, L. (2001) Thymidylate synthase expression in stage II and III colon cancer. Am. J. Clin. Oncol 24; 597-602.
18. Tsujitani, S., Konishi, I., Suzuki, K., Oka, S., Gomyo, Y., Matsumoto, S., Hirooka, Y. and Kaibara, N. (2000) Expression of thymidylate synthase in relation to survival and chemosensitivity in gastric cancer patients. J. Exp. Clin. Cancer Res. 19; 189-195.
19. Van Triest, B., Loftus, B. M., Pinedo, H. M., Backus, H. H. J., Schoenmakers, P., Telleman, F., Tadema, T., Aherne, G. W., Van Groeningen, C. J., Zoetmulder, F. A. N., Taal, B. G., Johnston, P. G. and Peters, G. J. (2000) Thymidylate synthase expression in patients with colorectal carcinoma using a polyclonal thymidylate synthase antibody in comparison to the TS 106 monoclonal antibody. J. Histochem. Cytochem. 48; 755-760.
20. Yamachika, T., Nakanishi, H., Inada, K., Tsukamoto, T., Kato, T., Fukushima, M., Inoue, M. and Tatematsu, M. (1998) A new prognostic factor for colorectal carcinoma, thymidylate synthase, and its therapeutic significance. Cancer 82; 70-77.