Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions

Biotechnology and Bioprocess Engineering

Volumn 4, Issue 4, 1999, Pages 253-258

  Sharp, Janet M ^a   Doran, Pauline M ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Antibody stability; Bacitracin; Hairy roots; Monoclonal antibody; Suspended plant cells

Indexed keywords

EID: 0001025735     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02933748     Document Type: Article

References (24)

1. Hiatt, A. C. (1990) The potential of antibodies in plants. Agbiotechnol. News Informat. 2: 653-655.
2. Hiatt, A., R. Cafferkey, and K. Bowdisk (1989) Production of antibodies in transgenic plants. Nature 342: 76-78.
3. De Neve, M., M. De Loose, A. Jacobs, H. Van Houdt, B. Kaluza, U. Weidle, M. Van Montagu, and A. Depicker (1993) Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis. Transgenic Res. 2: 227-237.
4. van Engelen, F. A., A. Schouten, J. W. Molthoff, J. Roosien, J. Salinas, W. G. Dirkse, A. Schots, J. Bakker, F. J. Gommers, M. A. Jongsma, D. Bosch, and W. J. Stiekema (1994) Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco. Plant Mole. Biol. 26: 1701-1710.
5. ab fragments in transgenic Arabidopsis thaliana plants. Plant Cell Physiol. 39: 639-646.
6. Khoudi, H., S. Laberge, J.-M. Ferullo, R. Bazin, A. Darveau, Y. Castonguay, G. Allard, R. Lemieux, and L.-P. Vézina (1999) Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol. Bioeng. 64: 135-143.
7. Ma, J. K.-C. (1995) Antibody expression in plants. p. 56-59 In: H. Y. Wang and T. Imanaka (eds.) Antibody Expression and Engineering. ACS Symp. Series 604. American Chemical Society. Washington, DC, U.S.A.
8. Wongsamuth, R. and P. M. Doran (1997) Production of monoclonal antibodies by tobacco hairy roots. Biotechnol. Bioeng. 54: 401-415.
9. Wahl, M. F., G. An, and J. M. Lee (1995) Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture. Biotechnol. Lett. 17: 463-468.
10. Magnuson, N. S., P. M. Linzmaier, J.-W. Gao, R. Reeves, G. An, and J. M. Lee (1996) Enhanced recovery of a secreted mammalian protein from suspension culture of genetically modified tobacco cells. Protein Exp. Pur. 7: 220-228.
11. LaCount, W., G. An, and J. M. Lee (1997) The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures. Biotechnol. Lett. 19: 93-96.
12. Wongsamuth, R. and P. M. Doran (1997) Hairy roots as an expression system for production of antibodies. p. 89-97 In: P. M. Doran (ed.) Hairy Roots: Culture and Applications. Harwood Academic. Amsterdam.
13. Siewert, G. and J. L. Strominger (1967) Bacitracin: an inhibitor of the dephosphorylation of lipid pyrophosphate, an intermediate in biosynthesis of the peptidoglycan of bacterial cell walls. Proc. Natl. Acad. Sci. U.S.A. 57: 767-773.
14. 2-macroglobulin and polypeptide hormones. Nature 283: 162-167.
15. van Noort, J. M., P. van den Berg, and I. E. Mattern (1991) Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic. Anal. Biochem. 198: 385-390.
16. Roth, R. A. (1981) Bacitracin: an inhibitor of the insulin degrading activity of glutathione-insulin transhydrogenase. Biochem. Biophys. Res. Commun. 98: 431-438.
17. 125I- insulin is associated with an increase in plasma membrane bound insulin and a potentiation of glucose oxidation by adipocytes. J. Biol. Chem. 257: 11563-11570.
18. Findlay, D. M., V. P. Michelangeli, J. M. Moseley, and T. J. Martin (1981) Calcitonin binding and degradation by two cultured human breast cancer cell lines (MCF 7 and T 47D). Biochem. J. 196: 513-520.
19. Bateman, K. S., M. Congiu, G. W. Tregear, A. E. Clarke, and M. A. Anderson (1997) Bacitracin significantly reduces degradation of peptides in plant cell cultures. Biotechnol. Bioeng. 53: 226-231.
20. 1 and IgA heavy chain domains in transgenic tobacco plants. Eur. J. Immunol. 24: 131-138.
21. Sharp, J. M. and P. M. Doran (1990) Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes. J. Biotechnol. 16: 171-186.
22. 2+ on submerged mycelial growth of Streptomyces azureus. J. Ferment. Bioeng. 86: 28-33.
23. Desbuquois, B., F. Krug, and P. Cuatrecasas (1974) Inhibitors of glucagon inactivation. Effect on glucagon-receptor interactions and glucagon-stimulated adenylate cyclase activity in liver cell membranes. Biochim. Biophys. Acta 343: 101-120.
24. Ericson, M. C., J. Gafford, and A. D. Elbein (1978) Bacitracin inhibits the synthesis of lipid-linked saccharides and glycoproteins in plants. Plant Physiol. 62: 373-376.