Sequential inactivation of reactive oxygen species by combined overexpression of SOD isoforms and catalase in insulin-producing cells

Free Radical Biology and Medicine

Volumn 34, Issue 6, 2003, Pages 683-688

  Lortz, Stephan ^a   Tiedge, Markus ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Autoimmune diabetes; Catalase; Insulin producing cells; Oxygen free radicals; RINm5F; Superoxide dismutase; cells

Indexed keywords

ANTIOXIDANT; CATALASE; COPPER ZINC SUPEROXIDE DISMUTASE; FREE RADICAL; HYDROGEN PEROXIDE; INSULIN; ISOENZYME; MANGANESE SUPEROXIDE DISMUTASE; MENADIONE; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE; XANTHINE OXIDASE;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; AUTOIMMUNE DISEASE; CELL PROTECTION; CONTROLLED STUDY; DIABETES MELLITUS; DISEASE COURSE; GENE OVEREXPRESSION; GENETIC TRANSFECTION; INSULIN SYNTHESIS; NONHUMAN; OXIDATIVE STRESS; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; TISSUE CULTURE; TOXICITY;

EID: 0037443701     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(02)01371-0     Document Type: Article

References (21)

1. Oberley L.W. Free radicals and diabetes. Free Radic. Biol. Med. 5:1988;113-124.
2. Horio F., Fukuda M., Katoh H., Petruzzelli M., Yano N., Rittershaus C., Bonner Weir S., Hattori M. Reactive oxygen intermediates in autoimmune islet cell destruction of the NOD mouse induced by peritoneal exudate cells (rich in macrophages) but not T cells. Diabetologia. 37:1994;22-31.
3. Lenzen S., Drinkgern J., Tiedge M. Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues. Free Radic. Biol. Med. 20:1996;463-466.
4. Tiedge M., Lortz S., Drinkgern J., Lenzen S. Relation between antioxidant enzyme gene expression and antioxidative defense status of insulin-producing cells. Diabetes. 46:1997;1733-1742.
5. Tiedge M., Lortz S., Munday R., Lenzen S. Complementary action of antioxidant enzymes in the protection of bioengineered insulin-producing RINm5F cells against the toxicity of reactive oxygen species. Diabetes. 47:1998;1578-1585.
6. Tiedge M., Lortz S., Munday R., Lenzen S. Protection against the cooperative toxicity of nitric oxide and oxygen free radicals by overexpression of antioxidant enzymes in bioengineered insulin-producing RINm5F cells. Diabetologia. 42:1999;849-855.
7. Fridovich I. Xanthine oxidase. Greenwald R.A. CRC handbook of methods for oxygen radical research. 1985;51-53 CRC Press, Boca Raton, FL.
8. Powis G. Free radical formation by antitumor quinones. Free Radic. Biol. Med. 6:1989;63-101.
9. Trujillo M., Alvarez M.N., Peluffo G., Freeman B.A., Radi R. Xanthine oxidase-mediated decomposition of S-nitrosothiols. J. Biol. Chem. 273:1998;7828-7834.
10. Mosmann T. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays . J. Immunol. Methods. 65:1983;55-63.
11. Lortz S., Tiedge M., Nachtwey T., Karlsen A.E., Nerup J., Lenzen S. Protection of insulin-producing RINm5F cells against cytokine-mediated toxicity through overexpression of antioxidant enzymes. Diabetes. 49:2000;1123-1130.
12. Korneluk R.G., Quan F., Lewis W.H., Guise K.S., Willard H.F., Holmes M.T., Gravel R.A. Isolation of human fibroblast catalase cDNA clones. Sequence of clones derived from spliced and unspliced mRNA. J. Biol. Chem. 259:1984;13819-13823.
13. Ho Y.S., Crapo J.D. cDNA and deduced amino acid sequence of rat copper-zinc-containing superoxide dismutase. Nucleic Acids Res. 15:1987;6746.
14. Beck Y., Oren R., Amit B., Levanon A., Gorecki M., Hartman J. Human Mn superoxide dismutase cDNA sequence. Nucleic Acids Res. 15:1987;9076.
15. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning a laboratory manual . 2nd ed :1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Nakamura M., Hayashi T. One- and two-electron reduction of quinones by rat liver subcellular fractions. J. Biochem. (Tokyo). 115:1994;1141-1147.
17. Rabinovitch A., Suarez Pinzon W.L., Strynadka K., Lakey J.R., Rajotte R.V. Human pancreatic islet β-cell destruction by cytokines involves oxygen free radicals and aldehyde production. J. Clin. Endocrinol. Metab. 81:1996;3197-3202.
18. Rabinovitch A., Suarez Pinzon W.L. Cytokines and their roles in pancreatic islet β-cell destruction and insulin-dependent diabetes mellitus. Biochem. Pharmacol. 55:1998;1139-1149.
19. Suarez Pinzon W.L., Strynadka K., Schulz R., Rabinovitch A. Mechanisms of cytokine-induced destruction of rat insulinoma cells the role of nitric oxide . Endocrinology. 134:1994;1006-1010.
20. Eizirik D.L., Mandrup-Poulsen T. A choice of death - the signal transduction of immune-mediated β-cell apoptosis. Diabetologia. 44:2001;2115-2133.
21. Hohmeier H.E., Thigpen A., Tran V.V., Davis R., Newgard C.B. Stable expression of manganese superoxide dismutase (MnSOD) in insulinoma cells prevents IL-1β-induced cytotoxicity and reduces nitric oxide production. J. Clin. Invest. 101:1998;1811-1820.