Colony lift assay using cell-coated filters: A fast and efficient method to screen phage libraries for cell-binding clones

Journal of Immunological Methods

Volumn 272, Issue 1-2, 2003, Pages 219-233

  Radošević, Katarina ^a,b   Voerman, Jane S A ^a   Hemmes, Annabrita ^a,b   Muskens, Femke ^a,b   Speleman, Luciënne ^a   De Weers, Michel ^a,b   Rosmalen, Judith G M ^a,c   Knegt, Paul ^a   Van Ewijk, Willem ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b CRUCELL HOLLAND BV   (Netherlands)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Cell coated filter; Colony lift; Phage library; scFv

Indexed keywords

SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ADHERENT CELL; ARTICLE; BACTERIUM COLONY; BIOASSAY; BIOFILTER; CELL GROWTH; CELL SPECIFICITY; CELL WALL; COLONY LIFT ASSAY; CONTROLLED STUDY; FLOW CYTOMETRY; GENE LIBRARY; HEAD AND NECK CARCINOMA; HUMAN; HUMAN CELL; LABORATORY; MOLECULAR CLONING; PRIORITY JOURNAL; SCREENING; SIGNAL DETECTION; TECHNIQUE;

ANIMALS; ANTIBODY SPECIFICITY; CELL LINE; CELLS, IMMOBILIZED; CLONE CELLS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOLOGIC TECHNIQUES; MICE; PEPTIDE LIBRARY;

BACTERIA (MICROORGANISMS);

EID: 0037438284     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00208-9     Document Type: Article

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