Purification of bacteriocin AS-48 from an Enterococcus faecium strain and analysis of the gene cluster involved in its production1

FEMS Microbiology Letters

Volumn 221, Issue 1, 2003, Pages 143-149

  Folli, Claudia ^a   Ramazzina, Ileana ^a   Arcidiaco, Patrizia ^b   Stoppini, Monica ^b   Berni, Rodolfo ^a  

^a UNIVERSITY OF PARMA   (Italy)

^b UNIVERSITY OF PAVIA   (Italy)

Author keywords

AS 48; Bacteriocin; Enterococcus faecium 7C5; PPD1 plasmid

Indexed keywords

BACTERIAL PROTEIN; BACTERIOCIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOSYNTHESIS; CONTROLLED STUDY; CYCLIZATION; DNA HYBRIDIZATION; ENTEROCOCCUS FAECIUM; GENE CLUSTER; GENE FUNCTION; GENETIC CODE; IMMUNITY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; STRUCTURAL GENE;

ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM;

EID: 0037432704     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00176-9     Document Type: Article

References (26)

1. Diep D.B., Nes I.F. Ribosomally synthesized antibacterial peptides in gram positive bacteria. Curr. Drug Targets. 3:2002;107-122.
2. Riley M.A., Wertz J.E. Bacteriocins: Evolution, Ecology, and Application. Annu. Rev. Microbiol. 56:2002;117-137.
3. van Kraaij C., de Vos W.M., Siezen R.J., Kuipers O.P. Lantibiotics: biosynthesis, mode of action and applications. Nat. Prod. Rep. 16:1999;575-587.
4. Cleveland J., Montville T.J., Nes I.F., Chikindas M.L. Bacteriocins: safe, natural antimicrobials for food preservation. Int. J. Food Microbiol. 71:2001;1-20.
5. Torri Tarelli G., Carminati D., Giraffa G. Production of bacteriocins active against Listeria monocytogenes and Listeria innocua from dairy enterococci. Food Microbiol. 11:1994;243-252.
6. Gálvez A., Maqueda M., Valdivia E., Quesada A., Montoya E. Characterization and partial purification of a broad spectrum antibiotic AS-48 produced by Streptococcus faecalis. Can. J. Microbiol. 32:1986;765-771.
7. Joosten H.M.L.J., Nuòez M., Devreese B., Van Beeumen J., Marugg J.D. Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Appl. Environ. Microbiol. 62:1996;4220-4223.
8. Maisnier-Patin S., Forni E., Richard J. Purification, partial characterisation and mode of action of enterococcin EFS2, an antilisterial bacteriocin produced by a strain of Enterococcus faecalis isolated from a cheese. Int. J. Food Microbiol. 30:1996;255-270.
9. Tomita H., Fujimoto S., Tanimoto K., Ike Y. Cloning and genetic and sequence analyses of the bacteriocin 21 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pPD1. J. Bacteriol. 179:1997;7843-7855.
10. Gonzales C., Langdon G.M., Bruix M., Gálvez A., Vadivia E., Maqueda M., Rico M. Bacteriocin AS-48, a microbial cyclic polypeptide structurally and functionally related to mammalian NK-lysin. Proc. Natl. Acad. Sci. USA. 97:2000;11221-11226.
11. Cobos E.S., Filimonov V.V., Gálvez A., Valdivia E., Maqueda M., Martínez J.C., Mateo P.L. The denaturation of circular enterocin AS-48 by urea and guanidinium hydrochloride. Biochim. Biophys. Acta. 1598:2002;98-107.
12. Cobos E.S., Filimonov V.V., Gálvez A., Maqueda M., Valdivia E., Martínez J.C., Mateo P.L. AS-48: a circular protein with an extremely stable globular structure. FEBS Lett. 505:2001;379-382.
13. Mann M., Meng C.K., Fenn J.B. Interpreting mass spectra of multiply charged ions. Anal. Chem. 61:1989;1702-1708.
14. Altschul M., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410.
15. Ehrenfeld E.E., Clewell D.B. Transfer functions of the Streptococcus faecalis plasmid pAD1: organization of plasmid DNA encoding response to sex pheromone. J. Bacteriol. 169:1987;3473-3481.
16. Martínez-Bueno M., Maqueda M., Gálvez A., Samyn B., Van Beeumen J., Coyette J., Vadivia E. Determination of the gene sequence and the molecular structure of the enterococcal peptide antibiotic AS-48. J. Bacteriol. 176:1994;6334-6339.
17. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
18. Martínez-Bueno M., Vadivia E., Gálvez A., Coyette J., Maqueda M. Analysis of the gene cluster involved in production and immunity of the peptide antibiotic AS-48 in Enterococcus faecalis. Mol. Microbiol. 27:1998;347-358.
19. Ehrenfeld E.E., Kessler R.E., Clewell D.B. Identification of pheromone-induced surface proteins in Streptococcus faecalis and evidence of a role for lipoteichoic acid in formation of mating aggregates. J. Bacteriol. 168:1986;6-12.
20. Nakayama J., Nagasawa H., Isogai A., Clewell D.B., Suzuki A. Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1. FEBS Lett. 268:1990;245-248.
21. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Samyn B., Martínez-Bueno M., Devreese B., Maqueda M., Gálvez A., Vadivia E., Coyette J., Van Beeumen J. The cyclic structure of the enterococcal peptide antibiotic AS-48. FEBS Lett. 352:1994;87-90.
23. Martínez-Bueno M., Gálvez A., Vadivia E., Maqueda M. A transferable plasmid associated with AS-48 production in Enterococcus faecalis. J. Bacteriol. 172:1990;2817-2818.
24. Fujimoto S., Tomita H., Wakamatsu E., Tanimoto K., Ike Y. Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response. J. Bacteriol. 177:1995;5574-5581.
25. Heaton M.P., Discotto L.F., Pucci M.J., Handwerger S. Mobilization of vancomycin resistance by transposon-mediated fusion of a VanA plasmid with an Enterococcus faecium sex pheromone-response plasmid. Gene. 171:1996;9-17.
26. Diaz M., Valdivia E., Martínez-Bueno M., Fernández M., Soler-González A.S., Ramírez-Rodrigo H., Maquedo M. Characterization of a new operon, as-48EFGH, from the as-48 gene cluster involved in immunity to enterocin AS-48. Appl. Environ. Microbiol. 69:2003;1229-1236.