Phe 771 of Escherichia coli DNA polymerase I (Klenow fragment) is the major site for the interaction with the template overhang and the stabilization of the pre-polymerase ternary complex

Biochemistry

Volumn 42, Issue 13, 2003, Pages 3645-3654

  Srivastava, Aashish ^a   Singh, Kamalendra ^a   Modak, Mukund J ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEXATION; CROSSLINKING; ENZYMES; ESCHERICHIA COLI; IONIC STRENGTH;

TERNARY COMPLEX;

DNA;

DNA; DNA DIRECTED DNA POLYMERASE BETA; ESCHERICHIA COLI PROTEIN; MUTANT PROTEIN; PHENYLALANINE; SODIUM CHLORIDE;

AMINO ACID SEQUENCE; ARTICLE; CHEMOSENSITIVITY; COLUMN CHROMATOGRAPHY; COMPLEX FORMATION; CONTROLLED STUDY; CROSS LINKING; DNA STRAND; DNA TEMPLATE; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; IONIC STRENGTH; ISOTOPE LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; RADIOACTIVITY;

BINDING SITES; CROSS-LINKING REAGENTS; DIDEOXYNUCLEOSIDES; DNA POLYMERASE I; DNA PRIMERS; ENZYME PRECURSORS; ENZYME STABILITY; ESCHERICHIA COLI; MACROMOLECULAR SUBSTANCES; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; PROTEIN CONFORMATION; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC; TRYPSIN; ULTRAVIOLET RAYS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0037426366     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi026699y     Document Type: Article

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