Inhibition of an activated Ras protein with genetically selected peptide aptamers

Biotechnology and Bioengineering

Volumn 82, Issue 1, 2003, Pages 38-46

  Kurtz, Stephen E ^a   Esposito, Kim ^b   Tang, Weimin ^b   Menzel, Rolf ^c  

^a PORTLAND VA MEDICAL CENTER   (United States)

^b BRISTOL MYERS SQUIBB   (United States)

^c Optigenix Inc   (United States)

Author keywords

Genetic suppressor; Peptide aptamer library; Ras; Saccharomyces cerevisiae

Indexed keywords

CONFORMATIONS; MODIFICATION; PROTEINS; YEAST;

HEAT SHOCK;

BIOTECHNOLOGY;

APTAMER; CYCLIC AMP; GUANOSINE TRIPHOSPHATE; RAS PROTEIN;

ALLELISM; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; FUNGUS GROWTH; GENE MUTATION; GENE REPRESSION; IN VIVO STUDY; NONHUMAN; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

BINDING SITES; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, SUPPRESSOR; LIGANDS; PEPTIDE LIBRARY; PEPTIDES; PROTEIN ENGINEERING; RAS PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037420409     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10538     Document Type: Article

References (47)

1. Abedi MR, Caponigro G, and Kamb A. 1998. Green fluorescent protein as a scaffold for intracellular presentation of peptides. Nucl Acids Res 26: 623-630.
2. Allen P, Worland S, and Gold L. 1995. Isolation of high affinity RNA ligands to HIV-1 integrase from a random pool. Virology 209: 327-336.
3. Anderegg RJ, Betz R, Carr SA, Crabb JW, Duntze W. 1988. Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component. J Biol Chem 263: 18236-40.
4. Blum JH, Dove SL, Hochschild A, Mekalanos JJ. 2000. Isolation of peptide aptamers that inhibit intracellular processes. Proc Natl Acad Sci USA 97:2241-2246.
5. Bock LC, Griffin LC, Latham JA, Vermaas EH, Toole JJ. 1992. Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature 355:564-566.
6. Broach JR. 1991. RAS genes in Saccharomyces cerevisiae: Signal transduction in search of a pathway. Trends Genet 7:28-33.
7. Broek D, Samiy N, Fasano O, Fujiyama A, Tamanoi F, Northup J, Wigler M. 1985. Differential activation of yeast adenylate cyclase by wildtype and mutant RAS proteins. Cell 41:763-769.
8. Colas P, Cohen B, Jessen T, Grishina I, McCoy J, Brent R. 1996. Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2. Nature 380:548-550.
9. Cull MG, Miller JF, Schatz PJ. 1992. Screening for receptor ligands using large libraries of peptides linked to the C terminus of the lac repressor. Proc Natl Acad Sci USA 89:1865-1869.
10. Cwirla SE, Balasubramanian P, Duffin DJ, Wagstrom CR, Gates CM, Singer SC, Davis AM, Tansik RL, Mattheakis LC, Boytos CM, Schatz PJ, Baccanari DP, Wrighton NC, Barrett RW, Dower WJ. 1997. Peptide agonist of the thrombopoietin receptor as potent as the natural cytokine. Science 276:1696-9.
11. Cwirla SE, Peters EA, Barrett RW, Dower WJ. 1990. Peptides on phage: A vast library of peptides for identifying ligands. Proc Natl Acad Sci USA 87:6378-6382.
12. DeFeo-Jones D, Scolnick EM, Koller R, Dhar R. 1983. ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae. Nature 306:707-709.
13. DeFeo-Jones D, Tatchell K, Robinson LC, Sigal IS, Vass WC, Lowy DR, Scolnick EM. 1985. Mammalian and yeast ras gene products: Biological function in their heterologous systems. Science 228:179-184.
14. Devlin JJ, Panganiban LC, Devlin PE 1990. Random peptide libraries: A source of specific protein binding molecules. Science 249:404-406.
15. Fabbrizio E, Le Cam L, Polanowska J, Kaczorek M, Lamb N, Brent R, Sardet C. 1999. Inhibition of mammalian cell proliferation by genetically selected peptide aptamers that functionally antagonize E2F activity. Oncogene 18:4357-4363.
16. Famulok M, Blind M, Mayer G. 2001. Intramers as promising new tools in functionalgenomics. Chem & Biol 8:931-939.
17. Fedor-Chaiken M, Deschenes RJ, Broach Jr. 1990. SRV2, a gene required for RAS activation of adenylate cyclase in yeast. Cell 61:329-340.
18. Fujiyama A, Tamanoi F. 1986. Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 83:1266-1270.
19. Gates CM, Stemmer WP, Kaptein R, Schatz PJ. 1996. Affinity selective isolation of ligands from peptide libraries through display on a lac repressor "headpiece dimer." J Mol Biol 255:373-386.
20. Giver L, Bartel DP, Zapp ML, Green MR, Ellington AD. 1993. Selective optimization of the Rev-binding element of HIV-1. Nucl Acids Res 21: 5509-5516.
21. Gold L, Polinsky B, Uhlenbeck OC, Yarus M. 1995 Diversity of oligonucleotide functions. Ann Rev Biochem 64:763-797.
22. Gudkov AV, Zelnick CR, Kazarov AR, Thimmapaya R, Suttle DP, Beck WT, Roninson IB. 1993. Isolation of genetic suppressor elements, inducing resistance to topoisomerase II-interactive cytotoxic drugs, from human topoisomerase II cDNA. Proc Natl Acad Sci USA 90: 3231-3235.
23. He B, Chen P, Chen SY, Vancura KL, Michaelis S, Powers S. 1991. RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proc Natl Acad Sci USA 88:11373-11377.
24. Herskowitz I. 1987. Functional inactivation of genes by dominant negative mutations. Nature 329:219-222.
25. Holmgren A. 1985. Thioredoxin. Ann Rev Biochem 54:237-271.
26. Kataoka T, Powers S, Cameron S, Fasano O, Goldfarb M, Broach J, and Wigler M. 1985. Functional homology of mammalian and yeast RAS genes. Cell 40:19-26.
27. Kataoka T, Powers S, McGill C, Fasano O, Strathern J, Broach J, Wigler M. 1984. Genetic analysis of yeast RAS1 and RAS2 genes. Cell 37: 437-445.
28. Kimoto M, Shirouza M, Mizutani S, Koide H, Kaziro Y, Hiroa I, Yokoyama S. 2002. Anti-(Raf-1) RNA aptamers that inhibit Ras-induced Raf-1 activation. Eur J Biochem 269:697-704.
29. Norman TC, Smith DL, Sorger PK, Drees BL, O'Rourke SM, Hughes TR, Roberts CJ, Friend SH, Fields S, Murray AW. 1999. Genetic selection of peptide inhibitors of biological pathways. Science 285:591-595.
30. Powers S, Gonzales E, Christensen T, Cubert J, and Broek D. 1991. Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant. Cell 65:1225-31.
31. Powers S, Kataoka T, Fasano O, Goldfarb M, Strathern J, Broach J, Wigler M. 1984. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. Cell 36:607-612.
32. Powers S, Michaelis S, Broek D, Santa Anna S, Field J, Herskowitz I, Wigler M. 1986. RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor. Cell 47:413-422.
33. Powers S, O'Neill K, Wigler M. 1989. Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae. Mol Cell Biol 9: 390-395.
34. Rose MD, Fink GR. 1987. KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast. Cell 48: 1047-1060.
35. Scott JK, Smith GP. 1990. Searching for peptide ligands with an epitope library. Science 249:386-390.
36. Sherman F, Fink GR, Hicks JB. 1986. Methods in yeast genetics. Cold Spring Harbor, NY: Cold Spring Harbor Press.
37. Shi H, Hoffman BE, Lis JT. 1999. RNA aptamers as effective protein antagonists in a multicellular organism. Proc Natl Acad Sci USA 96: 10033-1138.
38. Tanaka K, Nakafuku M, Satoh T, Marshall MS, Gibbs JB, Matsumoto K, Kaziro Y, Toh-e A. 1990. S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein. Cell 60:803-807.
39. Tang W, Ruknudin A, Yang WP, Shaw SY, Knickerbocker A, Kurtz S. 1995. Functional expression of a vertebrate inwardly rectifying K+ channel in yeast. Mol Biol Cell 6:1231-1240.
40. Taparowsky E, Suard Y, Fasano O, Shimizu K, Goldfarb M, Wigler M. 1982. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change. Nature 300:762-765.
41. Thomas M, Chedin S, Carles C, Riva M, Famoluk M, Sentenac A. 1997. Selective targeting and inhibition of yeast RNA polymerase II by RNA aptamers. J Biol Chem 272:27980-27986.
42. Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S, Broach J, Matsumoto K, Wigler M. 1985. In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell 40:27-36.
43. Tuerk C, Macdougal S, Gold L. 1992. RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase. Proc Natl Acad Sci USA 89:6988-6992.
44. Wrighton NC, Farrell FX, Chang R, Kashyap AK, Barbone FP, Mulcahy LS, Johnson DL, Barrett RW, Jolliffe LK, Dower WJ. 1996. Small peptides as potent mimetics of the protein hormone erythropoietin. Science 273:458-464.
45. Xu CW, Mendelsohn A, Brent R. 1997. Cells that register logical relationships among proteins. Proc Natl Acad Sci USA 94:12473-12478.
46. Yang M, Wu Z, Fields S. 1995. Protein-peptide interactions analyzed with the yeast two-hybrid system. Nucl Acids Res 23:1152-1156.
47. Yanofsky SD, Baldwin DN, Butler JH, Holden FR, Jacobs JW, Balasubramanian P, Chinn JP, Cwirla SE, Peters-Bhatt E, Whitehorn EA, Tate EH, Akeson A, Bowlin T, Dower WJ, Barrett RW. 1996. High affinity type I interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries. Proc Natl Acad Sci USA 93: 7381-7386.