Pulsed feeding during fed-batch fungal fermentation leads to reduced viscosity without detrimentally affecting protein expression

Biotechnology and Bioengineering

Volumn 81, Issue 3, 2003, Pages 341-347

  Bhargava, Swapnil ^a   Nandakumar, M P ^a   Roy, Anindya ^a   Wenger, Kevin S ^b   Marten, Mark R ^b  

^a UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^b North America Inc   (United States)

Author keywords

Agitation intensity; Fermentation; Filamentous fungi; Morphology; Pulse fraction; Rheology

Indexed keywords

BIOMASS; FERMENTATION; GLUCOSE; METABOLISM; MORPHOLOGY; PH EFFECTS; PROTEINS; VISCOSITY;

PROTEIN EXPRESSION;

FUNGI;

FUNGAL PROTEIN; GLUCOSE;

ARTICLE; ASPERGILLUS ORYZAE; BIOREACTOR; CONCENTRATION (PARAMETERS); CONTINUOUS FERMENTATION; CONTROLLED STUDY; CULTURE MEDIUM; FED BATCH FERMENTATION; FEEDING; FUNGAL METABOLISM; FUNGAL MORPHOLOGY; FUNGAL STRAIN; FUNGUS GROWTH; MICROBIAL BIOMASS; MYCELIUM; NONHUMAN; NUTRIENT; OXYGEN CONSUMPTION; PH; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; TECHNIQUE; VISCOSITY;

ASPERGILLUS ORYZAE; FUNGI;

EID: 0037419715     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10481     Document Type: Article

References (33)

1. Amanullah A, Blair R, Davies A, Riley GR, Thomas CR, Nienow AW. 1999. Effects of agitation intensity on mycelial morphology and protein production in chemostat cultures of recombinant Aspergillus oryzae. Biotechnol Bioeng 62:434-446.
2. Arora DK, Elander RP, Mukerji KG. 1992. Introduction. In: Arora DK, Elander RP, Mukerji KG, editors. Handbook of applied mycology, Vol 4:3-4. fungal biotechnology. New York: Marcel Dekker.
3. Bainbridge BW, Bull AT, Pirt SJ, Trinci APJ. 1971. Biochemical and structural changes in non-growing maintained and autolysing cultures of Aspergillus nidulans. Trans Br Mycol Soc 56:371-385.
4. Blanch HW, Bhavaraju SM. 1976. Bioengineering report: non-Newtonian fermentation broths - rheology and mass transfer. Biotechnol Bioeng 18:745-790.
5. Buckland BC, Gwewonyo K, DiMasi D, Hunt G, Westerfield G, Nienow AW. 1988. Improved performance in viscous mycelial fermentations by agitator retrofitting. Biotechnol Bioeng 31:737-742.
6. Carlsen M. 1994. α-Amylase production by Aspergillus oryzae. Ph.D. dissertation. Lyngby, Denmark: Technical University of Denmark.
7. Carlsen M, Nielsen J, Villadsen J. 1996a. Growth and α-amylase production by Aspergillus oryzae during continuous cultivation. J Biotechnol 45:81-93.
8. Carlsen M, Spohr AB, Nielsen J, Villadsen J. 1996b. Morphology and physiology of an α-amylase producing strain of Aspergillus oryzae during batch cultivations. Biotechnol Bioeng 49:266-276.
9. Charles M. 1978. Technical aspects of the rheological properties of microbial cultures. Adv Biochem Eng 8:1-62.
10. Gharahdaghi F, Weinberg C, Meagher D, Imai B, Mische S. 1999. Mass spectrometric identification of proteins from silver stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity. Electrophoresis 20:601-605.
11. Lahoz R, Reyes F, Alarcon GG, Criberio L, Lahoz-Beltra R. 1986. Behaviour of the cell walls of Aspergillus niger during the autolytic phase of growth. FEMS Microbiol Lett 36:265-268.
12. Li ZJ, Shukla V, Pedersen AG, Wenger KS, Fordyce AP, Marten MR. 2002. Estimation of hyphal tensile strength in production-scale Aspergillus oryzae fungal fermentations. Biotechnol Bioeng 77:601-613.
13. Marten MR, Wenger KS, Khan SA. 1997. Rheology, mixing time, and regime analysis for a production- scale Aspergillus oryzae fermentation. In: Nienow AW, editor. Bioreactor and bioprocess fluid dynamics. Cranfield, U.K.: BHR Group. P 295-313.
14. McIntyre M, Berry DR, McNeil B. 1999. Response of P. chrysogenum to oxygen starvation in glucose and nitrogen limited chemostat cultures. Enzyme Microb Tech 24:447-454.
15. McIntyre M, Berry DR, McNeil B. 2000. Role of proteases in autolysis of Penicillium chrysogenum chemostat cultures in response to nutrient depletion. Appl Microbiol Biotechnol 53:235-242.
16. McNeil B, Berry DR, Harvey LM, Grant A, White S. 1998. Measurement of autolysis in submerged batch cultures of Penicillium chrysogenum. Biotechnol Bioeng 57:297-305.
17. Metzner AB, Feehs RH, Ramos HL, Otto RE, Tuthill JD. 1961. Agitation of viscous Newtonian and non-Newtonian fluids. AIChE J 7:3-9.
18. Morkeberg R, Carlsen M, Nielsen J. 1995. Induction and repression of α-amylase production in batch and continuous cultures of Aspergillus oryzae. Microbiology 141:2449-2454.
19. Myers RH. 1990. Classical and modem regression with applications. Pacific Grove, CA: Duxbury Press.
20. Olsvik E, Kristiansen B. 1994. Rheology of filamentous fermentations. Biotechnol Adv 12:1-39.
21. Olsvik ES, Kristiansen B. 1992a. Influence of oxygen tension, biomass concentration, and specific growth rate on the rheological properties of a filamentous fermentation broth. Biotechnol Bioeng 40:1293-1299.
22. Olsvik ES, Kristiansen B. 1992b. On-line rheological measurements and control in fungal fermentations. Biotechnol Bioeng 40:375-387.
23. Paul GC, Thomas CR. 1998. Characterization of mycelial morphology using image analysis. Adv Biochem Eng 60:1-59.
24. Perez-Leblic MI, Reyes F, Lahoz R, Archer SA. 1982a. Autolysis of Penicillium oxalicum with special reference to its cell walls. Can J Microbiol 28:1289-1295.
25. Perez-Leblic MI, Reyes F, Martinez MJ, Lahoz R. 1982b. Cell wall degradation in the autolysis of filamentous fungi. Mycopathologia 80: 147-155.
26. Pitson S, Sevoiur RJ, Bott R, Stasinopoulos SJ. 1991. Production and regulation of β-glucanases in Acremonium and Cephalosporium isolates. Mycol Res 95:352-356.
27. 14C-L-aspartic acid. J Basic Microbiol 8: 475-482.
28. Rodriguez J, Copa-Patino JL, Perez-Leblic MI. 1995. Purification and properties of a chitinase from Penicillium oxalicum autolysates. Lett Appl Microbiol 20:46-49.
29. Sahai AS, Manocha MS. 1993. Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction. FEMS Microbiol Rev 11:317-338.
30. Smith JJ, Lilly MD, Fox RI. 1990. The effect of agitation on the morphology and penicillin production of Penicillium chrysogenum. Biotechnol Bioeng 35:1011-1023.
31. Spohr A, Dam-Mikkelsen C, Carlsen M, Nielsen J, Villadsen J. 1998. On-line study of fungal morphology during submerged growth in a small flow-through cell. Biotechnol Bioeng 58:541-553.
32. Taguchi H, Miyamoto S. 1966. Power requirement in non-Newtonian fermentation broth. Biotechnol Bioeng 8:43-54.
33. van Suijdam JC, Metz B. 1981. Influence of engineering variables upon the morphology of filamentous molds. Biotechnol Bioeng 23:111-148.