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Volumn 19, Issue 5, 2003, Pages 1766-1774

Planar supported lipid bilayer polymers formed by vesicle fusion. 2. Adsorption of bovine serum albumin

Author keywords

[No Author keywords available]

Indexed keywords

VESICLE FUSION;

EID: 0037418461     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la026198d     Document Type: Article
Times cited : (61)

References (82)
  • 10
    • 0035162637 scopus 로고    scopus 로고
    • (b) Puu, G. Anal. Chem. 2001, 73, 72-79.
    • (2001) Anal. Chem. , vol.73 , pp. 72-79
    • Puu, G.1
  • 23
    • 0032693817 scopus 로고    scopus 로고
    • (c) Bieri, C.; Ernst, O. P.; Heyse, S.; Hofmann, K. P.; Vogel, H. Nat. Biotechnol. 1999, 17, 1105-1106. Naumann, R.; Jonczyk, A.; Hampel, C.; Ringsdorf, H.; Knoll, W.; Bunjes, N.; Gräber, P. Bioelectrochem. Bioenerg. 1997, 42, 241-247.
    • (1999) Nat. Biotechnol. , vol.17 , pp. 1105-1106
    • Bieri, C.1    Ernst, O.P.2    Heyse, S.3    Hofmann, K.P.4    Vogel, H.5
  • 45
  • 61
    • 0003373776 scopus 로고
    • Proteins at interfaces II
    • Horbett, T. A., Brash, J. L., Eds.; American Chemical Society: Washington, DC
    • Some reviews on protein adsorption are: (a) Brash, J. L.; Horbett, T. A. In Proteins at Interfaces II; Horbett, T. A., Brash, J. L., Eds.; ACS Symposium Series 602; American Chemical Society: Washington, DC, 1995; pp 1-23.
    • (1995) ACS Symposium Series , vol.602 , pp. 1-23
    • Brash, J.L.1    Horbett, T.A.2
  • 65
    • 0344279935 scopus 로고    scopus 로고
    • note
    • As discussed previously by a number of authors (refs 22, 23, 26, and 27), we note that using TIRF to determine the surface coverage of an adsorbed protein film can be problematic. There are several contributing factors. Of most concern here is self-quenching of RITC fluorescence due to energy transfer, which may occur when RITC-labeled protein molecules are closely packed in an adsorbed film. This will cause the quantum yield to decrease as the surface coverage increases. Considering the fractional surface coverages presented in Table 1, the net effect of self-quenching is that the values listed for the most protein-resistant surfaces (e.g., POPC) are probably overestimated. Cognizant of these limitations, the data can still be quantitatively evaluated, allowing the protein resistance of (poly)PSLBs to be compared to the reference surfaces.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.