Self-assembled monolayers of alkanethiolates presenting mannitol groups are inert to protein adsorption and cell attachment

Langmuir

Volumn 16, Issue 24, 2000, Pages 9604-9608

  Luk, Yan Yeung ^a   Kato, Mihoko ^a   Mrksich, Milan ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; INTERFACES (MATERIALS); MONOLAYERS; OLIGOMERS; POLYETHYLENE GLYCOLS; PROTEINS; SPECTROSCOPIC ANALYSIS;

ALKANETHIOLATES; SELF ASSEMBLY MONOLAYERS (SAM); SURFACE PLASMON RESONANCE SPECTROSCOPY;

SULFUR COMPOUNDS;

EID: 0034317224     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la0004653     Document Type: Article

References (34)

1. (a) Mrksich, M. Cell Mol. Life Sci. 1998, 54, 653.
2. (b) Mrksich, M.; Whitesides, G. M. Annu. Rev. Biophys. Biomol. Struct. 1996, 25, 55-78.
3. (a) Prime, K. L.; Whitesides, G. D. J. Am. Chem. Soc. 1993, 115, 10714-10721.
4. (b) Prime, K. L.; Whitesides, G. M. Science 1991, 252, 1164-1167.
5. (a) Sigal, G. B.; Mrksich, M.; Whitesides, G. M. J. Am. Chem. Soc. 1998, 120, 3464-3473.
6. (b) Mrksich, M.; Whitesides, G. M. Chemistry and Biological Applications of Polyethylene Glycol. Am. Chem. Soc. Symp. Ser. 1997, 680, 361-373.
7. (a) Mrksich, M.; Dike, L. E.; Tien, J.; Ingber, D. E.; Whitesides, G. M. Exp. Cell Res. 1997 235, 305-313.
8. (b) Chen, C. S.; Mrksich, M.; Huang, S.; Whitesides, G. M.; Ingber, D. E. Science 1997, 276, 1425-1428.
9. (c) Mrksich, M.; Whitesides, G. M. TIBTECH. 1995, 13, 228-235.
10. Patterned alkylsiloxanes on hydroxylated substrates have been widely used to pattern cells. It remains unclear whether cells are confined by regions of substrate that are truly inert or rendered inert by the adsorption of protein. For examples, see (a) Jo, S.; Park, K. Biomaterials 2000, 21, 605-616.
11. (b) Saneinejad, S.; Shoichet, M. S. J. Biomed. Mater. Res. 1998, 42, 13-19.
12. (c) Murphy, E. F.; Lu, J. R.; Brewer, J.; Russell, J.; Penfold, J. Langmuir 1999, 15, 1313-1322.
13. (a) Mrksich, M.; Grunwell, J. R.; Whitesides, G. M. J. Am. Chem. Soc. 1995, 117, 12009-12010.
14. (b) Houseman, B. T.; Mrksich, M. Angew. Chem., Int. Ed. Engl. 1999, 38, 782-785.
15. (c) Spinke, J.; Liley, M.; Schmitt, F. J.; Guder, H. J.; Angermaier, L.; Knoll, W. J. Chem. Phys. 1993, 99, 7012-7019.
16. (a) Yousaf, M. N.; Mrksich, M. J. Am. Chem. Soc. 1999, 121, 4286-4287.
17. (b) Hodneland, C. H.; Mrksich, M. Langmuir 1997, 13, 6001-6003.
18. (c) Hodneland, C. H.; Mrksich, M. J. Am. Chem. Soc. 2000, 122, 4235-4236.
19. (d) Yousaf, M. N.; Houseman, B. T.; Mrksich, M. Submitted for publication.
20. Deng, L.; Mrksich, M.; Whitesides, G. M. J. Am. Chem. Soc. 1996, 118, 5136-5137.
21. 2 in ethanolic solutions of alkanethiol (2 mM) for 9 h. The SAMs were rinsed with ethanol and dried with nitrogen before use.
22. (a) Mrksich, M.; Sigal, G. B.; Whitesides, G. M. Langmuir 1995, 11, 4383-4385.
23. (b) Sigal, G. B.; Mrksich, M.; Whitesides, G. M. Langmuir 1997, 13, 2749-2755.
24. All SPR experiments were performed on a BIAcore 1000 instrument. Monolayer substrates were glued into plastic BIACore cassettes with a two-part epoxy (Devcon). Phosphate-buffered saline (PBS; 10 mM phosphate, 150 mM sodium chloride, pH 7.6) was degassed under vacuum, and all protein solutions were filtered through 0.45-μm filters before use.
25. Albino 3T3-Swiss fibroblasts (American Type Culture Collector) were harvested from culture dishes with trypsin-EDTA, washed, and resuspended in DMEM supplemented with 10% fetal bovine serum. The suspended cells were added to wells (10 000/ well) containing patterned monolayer substrates immersed in DMEM supplemented with serum. Serum-supplemented medium was exchanged with fresh medium after 12 h and then every 5 days. Cell cultures were maintained at 37 °C and photographed daily with a Hamamatsu CCD camera through a Zeiss axiovert phase-contrast microscope (5 × magnification).
26. Wieland, B.; Lancaster, J. P.; Hoaglund, C. S.; Holota, P.; Tornquist, W. J. Langmuir 1996, 12, 2594-2601.
27. (a) Harris, J. M. Poly(Ethyl Glycol) Chemistry; Plenum: New York, 1992.
28. (b) Jeon, S. I.; Andrade, J. D. J. Colloid Interface Sci. 1991, 142, 159-166.
29. (c) Taunton, H. J.; Toprakciohlu, C.; Fetters, L. J.; Klein, J. Nature 1988, 332, 712-714.
30. Harder, P.; Grunze, M.; Dahint, R.; Whitesides, G. M.; Laibinius, P. E. J. Phys Chem. B 1998, 102, 426-436.
31. Feldman, K.; Hahner, G.; Spencer, N. D.; Harder, P.; Grunze, M. J. Am. Chem. Soc. 1999, 121, 10134-10141.
32. Pertsin, A. J.; Grunze, M.; Garbuzova, I. J. Phys. Chem. B 1998, 102, 4918-4926.
33. Borkholder, D. A.; Bao, J.; Maluf, N. I.; Perl, E. R.; Kovacs, G. T. A. J. Neurosci. Methods 1997, 77, 61-66.
34. Kapur, R.; Giuliano, K. A.; Campana, M.; Adams, T.; Olson, K.; Jung, D.; Mrksich, M.; Vasudevan, C.; Taylor, D. L. Biomed. Microdevices 1999, 2, 99-109.