Inhibition of HCV NS3 protease by RNA aptamers in cells

Nucleic Acids Research

Volumn 31, Issue 7, 2003, Pages 1935-1943

  Nishikawa, Fumiko ^a   Kakiuchi, Nobuko ^a,c   Funaji, Kohei ^b   Fukuda, Kotaro ^a   Sekiya, Satoru ^a   Nishikawa, Satoshi ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b MITSUBISHI GAS CHEMICAL COMPANY INC   (Japan)

^c KANAZAWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIVIRUS AGENT; APTAMER; BETA ACTIN; CHIMERIC PROTEIN; CIS ACTING ELEMENT; GLOBIN; HELICASE; NONSTRUCTURAL PROTEIN 3; PROTEINASE; PROTEINASE INHIBITOR; RIBOZYME; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA; VIRUS VECTOR;

ANTIVIRAL ACTIVITY; CELL CULTURE; CONTROLLED STUDY; CYTOMEGALOVIRUS; ENHANCER REGION; ENZYME ACTIVITY; ENZYME INHIBITION; GENE CONSTRUCT; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; HEPATITIS C VIRUS; HEPATITIS DELTA VIRUS; HUMAN; HUMAN CELL; HUMAN GENOME; IN VITRO STUDY; IN VIVO STUDY; MUTANT; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; RETROVIRUS; REVIEW; RNA STABILITY; RNA TRANSCRIPTION; TANDEM REPEAT; VIRAL GENE DELIVERY SYSTEM;

BASE SEQUENCE; BINDING, COMPETITIVE; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; OLIGORIBONUCLEOTIDES; PLASMIDS; RNA, CATALYTIC; SERINE ENDOPEPTIDASES; TANDEM REPEAT SEQUENCES; TRANSFECTION; TUMOR CELLS, CULTURED; VIRAL NONSTRUCTURAL PROTEINS;

CYTOMEGALOVIRUS; GALLUS GALLUS; HEPATITIS C VIRUS; HEPATITIS DELTA VIRUS; RNA VIRUSES; TYPE D RETROVIRUSES; UNIDENTIFIED RETROVIRUS;

EID: 0037390892     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg291     Document Type: Review

References (27)

1. Rosenberg,S. (2001) Recent advances in the molecular biology of hepatitis C virus. J. Mol. Biol., 313, 451-464.
2. De Francesco,R., Pessi,A. and Steinkuhler,C. (1998) The hepatitis C virus NS3 proteinase: structure and function of a zinc-containing serine proteinase. Antiviral Ther., 3, 99-109.
3. Dymock,B.W., Jones,P.S. and Wilson,F.X. (2001) Novel approaches to the treatment of hepatitis C virus infection. Antiviral Chem. Chemother., 11, 79-96.
4. Bianchi,E. and Pessi,A. (2002) Inhibiting viral proteases: challenges and opportunities. Biopolymers, 66, 101-114.
5. Fukuda,K., Vishnuvardhan,D., Sekiya,S., Hwang,J., Kakiuchi,N., Taira,K., Shimotohno,K., Kumar,P.K.R. and Nishikawa,S. (2000) Isolation and characterization of RNA aptamers specific for the hepatitis C virus NS3 protease. Eur. J. Biochem., 267, 3685-3694.
6. Hwang,J., Fauzi,H., Fukuda,K., Sekiya,S., Kakiuchi,N., Shimotohno,K., Taira,K., Kusakabe,I. and Nishikawa,S. (2000) The RNA aptamer-binding site of hepatitis C virus NS3 protease. Biochem. Biophys. Res. Commun., 279, 557-562.
7. Yao,N., Reichert,P., Taremi,S.S., Prosise,W.W. and Weber,P.C. (1999) Molecular views of viral polyprotein processing revealed by the crystal structure of the hepatitis C virus bifunctional protease-helicase. Struct. Fold. Design, 15, 1353-1363.
8. Nishikawa,S., Hwang,J., Fauzi,H., Fukuda,K., Nishikawa,F. and Kakiuchi,N. (2002) Aptamers (nucleic acids ligands) for trypsin-like serine proteases: finding the enzyme's Achilles heel. Curr. Topics Anal. Chem., 3, 69-81
9. Vishnuvardhan,D., Kakiuchi,N., Urvil,P.T., Shimotohno,K., Kumar,P.K.R. and Nishikawa,S. (1997) Expression of highly active recombinant NS3 protease domain of hepatitis C virus in E. coli. FEBS Lett., 402, 209-212.
10. Hirowatari,Y., Hijikata,M. and Shimotohno,K. (1995) A novel method for analysis of viral proteinase activity encoded by hepatitis C virus in cultured cells. Anal. Biochem, 225, 113-120.
11. Fauzi,H., Chiba,A., Nishikawa,F., Roy,M., Kawakami,J. and Nishikawa,S. (1998) Minimization of genomic human hepatitis delta virus ribozyme. Anal. Chim. Acta, 365, 309-317.
12. Kawakami,J., Kumar,P.K.R., Suh,Y.-A., Nishikawa,F., Kawakami,K., Taira,K. and Nishikawa,S. (1993) Identification of important bases in a single-stranded region (SSrC) of the hepatitis delta (δ) virus ribozyme. Eur. J. Biochem., 217, 29-36.
13. Niwa,H., Yamanuma,K. and Miyazaki,J. (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108, 193-200.
14. Nishikawa,F., Roy,M., Fauzi,H. and Nishikawa,S. (1999) Detailed analysis of stem I and its 5′ and 3′ neighbor regions in the trans-acting HDV ribozyme. Nucleic Acids Res., 27, 403-410.
15. Kakiuchi,N., Hijikata,M., Komoda,Y., Tanji,Y., Hirowatari,Y. and Shimotohno,K. (1995) Bacterial expression and analysis of cleavage activity of HCV serine proteinase using recombinant and synthetic substrate. Biochem. Biophys. Res. Commun., 210, 1059-1065.
16. Kunkel,G.R., Maser,R.L., Calvet,J.P. and Pederson,T. (1986) U6 small nuclear RNA is trnanscribed by RNA polymerase III. Proc. Natl Acad. Sci. USA, 83, 8575-8579.
17. Ferré-D'Amaré,A.R., Zhou,K. and Doudna,J.A. (1998) Crystal structure of a hepatitis delta virus ribozyme, Nature, 395, 567-574.
18. Lévesque,D., Choufani,S. and Perreault,J.-P. (2002) Delta ribozyme benefits from a good stability in vitro that become outstanding in vivo. RNA, 8, 464-477.
19. Suh,Y.A., Kumar,P.K.R., Kawakami,J., Nishikawa,F., Taira,K. and Nishikawa,S. (1993) Systematic substitution of individual bases in two important single-stranded regions of the HDV ribozyme for evaluation of the role of specific bases. FEBS Lett., 326, 158-162.
20. Wolk,B., Sansonno,D., Krausslich,H.-S., Dammacco,F., Rice,C.M., Blum,H.E. and Moradpour,D. (2000) Subcellular localization, stability and trans-cleavage competence of the Hepatitis C Virus NS3-NS4A complex expressed in tetracycline-regulated cell lines. J. Virol., 74, 2293-2304.
21. Bachi,A., Braun,I.C., Rodrigues,J.P., Pante,N., Ribbeck,K., Kobbe,C.V., Kutay,U., Wilm,M., Gorlich,D., Carmo-Fonseca,M. and Izaurralde,E. (2000) The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates. RNA, 6, 136-158.
22. Gruter,P., Tabernero,C., Kobbe,C.V., Schmitt,C., Saaverdra,C., Bachi,A., Wilm,M., Felber,B.K and Izaurralde,E. (1998) TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol. Cell, 1, 649-659.
23. Ohkawa,J., Yuyama,N., Takebe,Y., Nishikawa,S. and Taira,K. (1993) Importance of independence in ribozyme reactions: kinetic behavior of trimmed and simply connected multiple ribozymes with potential activity against human immunodeficiency virus. Proc. Natl Acad. Sci. USA, 90, 11302-11306.
24. Been,M.D. and Wickham,G.S. (1997) Self cleaving ribozymes of hepatitis delta virus RNA. Eur. J. Biochem., 247, 741-753.
25. Werstuck,G. and Green,MR. (1998) Controlling gene expression in living cells through small molecule-RNA interactions. Science, 282, 296-298.
26. Shi,H., Hoffman,E. and Lis,J.T. (1999) RNA aptamers as effective protein antagonists in a multicellular organism. Proc. Natl Acad. Sci. USA, 96, 10033-10038.
27. Makino,S., Chang,M.-F., Shieh,C.-K., Kamahora,T., Vannier,D., Govindarajan,S. and Lai,M.M.C. (1987) Molecular cloning and sequence of a human delta virus RNA. Nature, 329, 343-346.