Putative role for EPC-1/PEDF in the G0 growth arrest of human diploid fibroblasts

Journal of Cellular Physiology

Volumn 195, Issue 1, 2003, Pages 12-20

  Pignolo, Robert J ^a,b   Kay Francis, Mary ^a,c   Rotenberg, Mitch O ^a,c   Cristofalo, Vincent J ^a,c,d  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c LANKENAU INSTITUTE FOR MEDICAL RESEARCH   (United States)

^d THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NEUTRALIZING ANTIBODY; PIGMENT EPITHELIUM DERIVED FACTOR; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANTIBODY PRODUCTION; ARTICLE; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL MIGRATION; CELL POPULATION; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED STUDY; CULTURE MEDIUM; DIPLOIDY; DNA ISOLATION; DNA SYNTHESIS; FIBROBLAST; GENETIC TRANSCRIPTION; GROWTH INHIBITION; HUMAN; HUMAN CELL; IN VITRO STUDY; INHIBITION KINETICS; MOLECULAR RECOGNITION; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SIMIAN VIRUS; STEADY STATE;

ANTIBODIES; BLOTTING, NORTHERN; BLOTTING, WESTERN; CELL AGING; CELL DIVISION; CELL LINE; CELL LINE, TRANSFORMED; CULTURE MEDIA, CONDITIONED; DIPLOIDY; DNA; EYE PROTEINS; FIBROBLASTS; G0 PHASE; GROWTH SUBSTANCES; HUMANS; IMMUNE SERA; NERVE GROWTH FACTORS; PROTEINS; RECOMBINANT PROTEINS; SERPINS; SIMIAN VIRUS 40;

SIMIAE; SIMIAN VIRUS 40; TRIXIS;

EID: 0037375555     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcp.10212     Document Type: Article

References (59)

1. Alberdi E, Hyde CC, Becerra SP. 1998. Pigment epithelium-derived factor (PEDF) binds to glycosaminoglycans: Analysis of the binding site. Biochemistry 37:10643-10652.
2. Alberdi E, Aymerich MS, Becerra SP. 1999. Binding of pigment epithelium-derived factor (PEDF) to retinoblastoma cells and cerebellar granule neurons. Evidence for a PEDF receptor. J Biol Chem 274:31605-31612.
3. Araki T, Taniwaki T, Becerra SP, Chader GJ, Schwartz JP. 1998. Pigment epithelium-derived factor (PEDF) differentially protects immature but not mature cerebellar granule cells against apoptotic cell death. J Neurosci Res 53:7-15.
4. Ausubel FM. 1988. Current protocols in molecular biology. J. Wiley, New York: Greene Pub. Associates and Wiley-Interscience. p 2.2.2-2.2.4.
5. Bilak MM, Corse AM, Bilak SR, Lehar M, Tombran-Tink J, Kuncl RW. 1999. Pigment epithelium-derived factor (PEDF) protects motor neurons from chronic glutamate-mediated neurodegeneration. J Neuropathol Exp Neurol 58:719-728.
6. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-524.
7. Cao W, Tombran-Tink J, Chen W, Mrazek D, Elias R, McGinnis JF. 1999. Pigment epithelium-derived factor protects cultured retinal neurons against hydrogen peroxide-induced cell death. J Neurosci Res 57:789-800.
8. Cao W, Tombran-Tink J, Elias R, Sezate S, Mrazek D, McGinnis JF. 2001. In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor. Invest Ophthalmol Vis Sci 42: 1646-1652.
9. Cayouette M, Smith SB, Becerra SP, Gravel C. 1999. Pigment epithelium-derived factor delays the death of photoreceptors in mouse models of inherited retinal degenerations. Neurobiol Dis 6:523-532.
10. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
11. Cohen J, Sugita Y, Chader GJ, Schwartz JP. 2000. Recombinant forms of the neurotrophic factor pigment epithelium-derived factor activate cellular metabolism and inhibit proliferation of the RAW macrophage cell line. Neuroimmunomodulation 7:51-58.
12. Coljee VW, Rotenberg MO, Tresini M, Francis MK, Cristofalo VJ, Sell C. 2000. Regulation of EPC-1/PEDF in normal human fibroblasts is posttranscriptional. J Cell Biochem 79:442-452.
13. Crawford SE, Stellmach V, Ranalli M, Huang X, Huang L, Volpert O, De Vries GH, Abramson LP, Bouck N. 2001. Pigment epithelium-derived factor (PEDF) in neuroblastoma: A multifunctional mediator of Schwann cell antitumor activity. J Cell Sci 114:4421-4428.
14. Cristofalo VJ. 1976. Thymidine labelling index as a criterion of aging in vitro. Gerontology 22:9-27.
15. Cristofalo VJ, Charpentier R. 1980. A standard procedure for cultivating human diploid fibroblasts to study cellular aging. J Tissue Cult Meth 6:117-122.
16. Cristofalo VJ, Sharf BB. 1973. Cellular senescence and DNA synthesis. Thymidine incorporation as a measure of population age in human diploid cells. Exp Cell Res 76:419-427.
17. Dawson DW, Volpert OV, Gillis P, Crawford SE, Xu H, Benedict W, Bouck NP. 1999. Pigment epithelium-derived factor: A potent inhibitor of angiogenesis. Science 285:245-248.
18. DeCoster MA, Schabelman E, Tombran-Tink J, Bazan NG. 1999. Neuroprotection by pigment epithelial-derived factor against glutamate toxicity in developing primary hippocampal neurons. J Neurosci Res 56:604-610.
19. Del Sal G, Ruaro ME, Philipson L, Schneider C. 1992. The growth arrest-specific gene, gas1, is involved in growth suppression. Cell 70:595-607.
20. DiPaolo BR, Pignolo RJ, Cristofalo VJ. 1995. Identification of proteins differentially expressed in quiescent and proliferatively senescent fibroblast cultures. Exp Cell Res 220:178-185.
21. Gao G, Li Y, Zhang D, Gee S, Crosson C, Ma J. 2001. Unbalanced expression of VEGF and PEDF in ischemia-induced retinal neovascularization. FEBS Lett 489:270-276.
22. Gao G, Li Y, Gee S, Dudley A, Fant J, Crosson CE, Ma JX. 2002. Downregulation of VEGF and up-regulation of PEDF: A possible mechanism for the anti-angiogenic activity of plasminogen Kringle 5. J Biol Chem.
23. Glisin V, Crkvenjakov R, Byus C. 1974. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13:2633-2637.
24. Hayflick L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 25:614-636.
25. Hjelmeland LM, Cristofolo VJ, Funk W, Rakoczy E, Katz ML. 1999. Senescence of the retinal pigment epithelium. Mol Vis 5:33.
26. Houenou LJ, D'Costa AP, Li L, Turgeon VL, Enyadike C, Alberdi E, Becerra SP. 1999. Pigment epithelium-derived factor promotes the survival and differentiation of developing spinal motor neurons. J Comp Neurol 412:506-514.
27. Kozaki K, Miyaishi O, Koiwai O, Yasui Y, Kashiwai A, Nishikawa Y, Shimizu S, Saga S. 1998. Isolation, purification, and characterization of a collagen-associated serpin, caspin, produced by murine colon adenocarcinoma cells. J Biol Chem 273:15125-15130.
28. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
29. Mori K, Duh E, Gehlbach P, Ando A, Takahashi K, Pearlman J, Mori K, Yang HS, Zack DJ, Ettyreddy D, Brough DE, Wei LL, Campochiaro PA. 2001. Pigment epithelium-derived factor inhibits retinal and choroidal neovascularization. J Cell Physiol 188:253-263.
30. Nichols WW, Murphy DG, Cristofalo VJ, Toji LH, Greene AE, Dwight SA. 1977. Characterization of a new human diploid cell strain, IMR-90. Science 196:60-63.
31. Nomura T, Yabe T, Mochizuki H, Reiser J, Becerra SP, Schwartz JP. 2001. Survival effects of pigment epithelium-derived factor expressed by a lentiviral vector in rat cerebellar granule cells. Dev Neurosci 23:145-152.
32. Ogata N, Wang L, Jo N, Tombran-Tink J, Takahashi K, Mrazek D, Matsumura M. 2001. Pigment epithelium derived factor as a neuroprotective agent against ischemic retinal injury. Curr Eye Res 22:245-252.
33. Ohno-Matsui K, Morita I, Tombran-Tink J, Mrazek D, Onodera M, Uetama T, Hayano M, Murota SI, Mochizuki M. 2001. Novel mechanism for age-related macular degeneration: An equilibrium shift between the angiogenesis factors VEGF and PEDF. J Cell Physiol 189:323-333.
34. Palmieri D, Watson JM, Rinehart CA. 1999. Age-related expression of PEDF/EPC-1 in human endometrial stromal fibroblasts: Implications for interactive senescence. Exp Cell Res 247:142-147.
35. Phillips PD, Cristofalo VJ. 1980. A procedure for the serum-free growth of normal human diploid fibroblasts. J Tissue Culture Meth 6:123-126.
36. Phillips NJ, Ziegler MR, Radford DM, Fair KL, Steinbrueck T, Xynos FP, Donis-Keller H. 1996. Allelic deletion on chromosome 17p13.3 in early ovarian cancer. Cancer Res 56:606-611.
37. 0 state. J Biol Chem 268:8949-8957.
38. Pignolo RJ, Rotenberg MO, Cristofalo VJ. 1994. Alterations in contact and density-dependent arrest state in senescent WI-38 cells. In Vitro Cell Dev Biol Anim 30A:471-476.
39. Pignolo RJ, Rotenberg MO, Cristofalo VJ. 1995. Analysis of EPC-1 growth state-dependent expression, specificity, and conservation of related sequences. J Cell Physiol 162:110-118.
40. 0 state. Exp Gerontol 33:67-80.
41. Rose TM, Tremblay S, Khandjian EW. 1992. Repression of a G0-associated 65-kDa protein in actively proliferating and SV40-transformed mouse kidney cells. Biochem Cell Biol 70:149-155.
42. Rubelj I, Pereira-Smith OM. 1994. SV40-transformed human cells in crisis exhibit changes that occur in normal cellular senescence. Exp Cell Res 211:82-89.
43. Sambrook J, Maniatis T, Fritsch EF. 1989. Molecular cloning a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. p 9.38-9.44.
44. Schneider C, King RM, Philipson L. 1988. Genes specifically expressed at growth arrest of mammalian cells. Cell 54:787-793.
45. Severino J, Francis MK, Cristofalo VJ. 1997. Expression of the EPC-1 gene and its role in the etiology of ovarian carcinoma. 27th Annual Meeting of the American Aging Association.
46. Shay JW, Van Der Haegen BA, Ying Y, Wright WE. 1993. The frequency of immortalization of human fibroblasts and mammary epithelial cells transfected with SV40 large T-antigen. Exp Cell Res 209:45-52.
47. Smith DE, Fisher PA. 1984. Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: Application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots. J Cell Biol 99:20-28.
48. Steele FR, Chader GJ, Johnson LV, Tombran-Tink J. 1993. Pigment epithelium-derived factor: Neurotrophic activity and identification as a member of the serine protease inhibitor gene family. Proc Natl Acad Sci USA 90:1526-1530.
49. Stellmach V, Crawford SE, Zhou W, Bouck N. 2001. Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor. Proc Natl Acad Sci USA 98:2593-2597.
50. Stuber D, Bannwarth W, Pink JR, Meloen RH, Matile H. 1990. New B cell epitopes in the Plasmodium falciparum malaria circumsporozoite protein. Eur J Immunol 20:819-824.
51. Suggs SV, Wallace RB, Hirose T, Kawashima EH, Itakura K. 1981. Use of synthetic oligonucleotides as hybridization probes: Isolation of cloned cDNA sequences for human beta 2-microglobulin. Proc Natl Acad Sci USA 78:6613-6617.
52. Sugita Y, Becerra SP, Chader GJ, Schwartz JP. 1997. Pigment epithelium-derived factor (PEDF) has direct effects on the metabolism and proliferation of microglia and indirect effects on astrocytes. J Neurosci Res 49:710-718.
53. Taniwaki T, Becerra SP, Chader GJ, Schwartz JP. 1995. Pigment epithelium-derived factor is a survival factor for cerebellar granule cells in culture. J Neurochem 64:2509-2517.
54. Taniwaki T, Hirashima N, Becerra SP, Chader GJ, Etcheberrigaray R, Schwartz JP. 1997. Pigment epithelium-derived factor protects cultured cerebellar granule cells against glutamate-induced neurotoxicity. J Neurochem 68:26-32.
55. Tombran-Tink J, Pawar H, Swaroop A, Rodriguez I, Chader GJ. 1994. Localization of the gene for pigment epithelium-derived factor (PEDF) to chromosome 17p13.1 and expression in cultured human retinoblastoma cells. Genomics 19:266-272.
56. Tombran-Tink J, Shivaram SM, Chader GJ, Johnson LV, Bok D. 1995. Expression, secretion, and age-related downregulation of pigment epithelium-derived factor, a serpin with neurotrophic activity. J Neurosci 15:4992-5003.
57. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354.
58. Tresini M, Pignolo RJ, Allen RG, Cristofalo VJ. 1999. Effects of donor age on the expression of a marker of replicative senescence (EPC-1) in human dermal fibroblasts. J Cell Physiol 179:11-17.
59. Wang H, Xie Z, Scott RE. 1996. Differentiation modulates the balance of positive and negative Jun/AP-1 DNA binding activities to regulate cellular proliferative potential: Different effects in nontransformed and transformed cells. J Cell Biol 135:1151-1162.