Expression of a functional recombinant Phoneutria nigriventer toxin active on K+ channels

Toxicon

Volumn 41, Issue 3, 2003, Pages 305-313

  Carneiro, A M D ^a   Kushmerick, C ^a   Koenen, J ^a   Arndt, M H L ^a   Cordeiro, M N ^a   Chavez Olortegui, C ^a   Diniz, C R ^a   Gomez, M V ^a   Kalapothakis, E ^a   Prado, M A M ^a   Prado, V F ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

GH3 cells; K+ channels; Phoneutria nigriventer; Spider toxins

Indexed keywords

AMMONIA; PHONEUTRIA NIGRIVENTER TOXIN; POTASSIUM CHANNEL; SPIDER VENOM; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BACTERIUM; CONTROLLED STUDY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR BIOLOGY; NONHUMAN; PHONEUTRIA NIGRIVENTER; PRIORITY JOURNAL; PURIFICATION; RAT; SPIDER; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; MOLECULAR SEQUENCE DATA; NEUROPEPTIDES; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; RECOMBINANT FUSION PROTEINS; SPIDER VENOMS; TUMOR CELLS, CULTURED;

ARANEAE; BACTERIA (MICROORGANISMS); PHONEUTRIA; PHONEUTRIA NIGRIVENTER;

EID: 0037355449     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(02)00292-1     Document Type: Article

References (51)

1. + channel revealed by the NMR-derived structures of scorpion toxins. Neuron. 15:1995;1169-1181.
2. Araújo D.A.M., Cordeiro M.N., Diniz C.R., e Beirão P.S.L. Effects of a toxic fraction, Phtx2, from the spider Phoneutria nigriventer on the sodium channel. Curr. Arch. Pharmacol. 347:1993;205.
3. Armstrong C.M., Benzanilla F.J. Charge movement associated with the opening and closing of the activation gates of the Na channels. J. Gen. Physiol. 63:1974;533-552.
4. + channels by a novel scorpion toxin. Nature. 296:1982;90-91.
5. Carlier E., Fajloun Z., Mansuelle P., Fathallah M., Mosbah A., Oughideni R., Sandoz G., Di Luccio E., Geib S., Regaya I., Brocard J., Rochat H., Darbon H., Devaux C., Sabatier J.M., de Waard M. Disulfide bridge reorganization induced by proline mutations in maurotoxin. FEBS Lett. 489:2001;202-207.
6. Cordeiro M.N., Figueiredo S.G., Valentim A.C., Diniz C.R., von Eickstedt V.R.D., Gilroy J., Richardson M. Purification and amino acid sequences of six TX3 type neurotoxins from the venom of the brazilian armed spider Phoneutria nigriventer. Toxicon. 31:1993;35-42.
7. Diniz C.R. Separation of proteins and characterization of active substances in the venom of the Brazilian spiders. Anais Acad. Bras. Cien. 35:1963;283-291.
8. v3.4. J. Biol. Chem. 273:1998;6744-6749.
9. Escoubas P., Diochot S., Corzo G. Structure and pharmacology of spider venom neurotoxins. Biochimie. 82:2000;893-907.
10. Figueiredo S.G., Garcia M.E., Valentim A.C., Cordeiro M.N., Diniz C.R., Richardson M. Purification and amino acid sequence of the insecticidal neurotoxin Tx4 (6-1) from the venom of the armed spider Phoneutria nigriventer. Toxicon. 33:1995;83-93.
11. Figueiredo S.G., de Lima M.E., Nascimento Cordeiro M., Diniz C.R., Patten D., Halliwell R.F., Gilroy J., Richardson M. Purification and amino acid sequence of a highly insecticidal toxin from the venom of the brazilian spider Phoneutria nigriventer which inhibits NMDA-evoked currents in rat hippocampal neurones. Toxicon. 39:2001;309-317.
12. Garcia M.L., Hanner M., Knaus H.G., Slaughter R., Kaczorowski G.J. Scorpion toxins as tools for studying potassium channels. Meth. Enzymol. 294:1999;624-639.
13. Giuliani C.D., Iemma M.R., Bondioli A.C., Souza D.H., Ferreira L.L., Amaral A.C., Salvini T.F., Selistre-de-Araujo H.S. Expression of an active recombinant lysine 49 phospholipase A(2) myotoxin as a fusion protein in bacteria. Toxicon. 39:2001;1595-1600.
14. + channel: peptide and channel residues mediating molecular recognition. Neuron. 12:1994;1377-1388.
15. v1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines. Mol. Pharmacol. 45:1994;1227-1234.
16. Gross A., MacKinnon R. Agitoxin footprinting the shaker potassium channel pore. Neuron. 6:1996;399-406.
17. Guan C., Li P., Riggs P.D., Inouye H. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene. 67:1988;21-30.
18. Guatimosim C., Romano-Silva M.A., Cruz J.S., Beirao P.S., Kalapothakis E., Moraes-Santos T., Cordeiro M.N., Diniz C.R., Gomez M.V., Prado M.A. A toxin from the spider Phoneutria nigriventer that blocks calcium channels coupled to exocytosis. Br. J. Pharmacol. 122:1997;591-597.
19. Hamill O.P., Marty A., Neher E., Sakmann B., Sigworth F.J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391:1981;85-100.
20. + channel pore through mutant cycles with a peptide inhibitor. Science. 268:1995;307-310.
21. Imredy J.P., MacKinnon R. Energetic and structural interactions between delta-dendrotoxin and a voltage-gated potassium channel. J. Mol. Biol. 296:(5):2000;1283-1294.
22. + channel. J. Biol. Chem. 275:2000;24639-24644.
23. Kalapothakis E., Penaforte C.L., Beirao P.S., Romano-Silva M.A., Cruz J.S., Prado M.A., Guimaraes P.E., Gomez M.V., Prado V.F. Cloning of cDNAS encoding neurotoxic peptides from the spider Phoneutria nigriventer. Toxicon. 36:1998;1843-1850.
24. 3 cells. J. Neurochem. 72:1999;1472-1481.
25. Leao R.M., Cruz J.S., Diniz C.R., Cordeiro M.N., Beirao P.S. Inhibition of neuronal high-voltage activated calcium channels by the omega-Phoneutria nigriventer Tx3-3 peptide toxin. Neuropharmacology. 39:2000;1756-1767.
26. + channel blocker purified from Androctonus australis scorpion venom. FEBS Lett. 417:1997;123-129.
27. MacKinnon R. Nothing automatic about ion-channel structures. Nature. 416:2002;261-262.
28. + channels from mammalian skeletal muscle. Nature. 313:1985;316-318.
29. Miranda D.M., Romano-Silva M.A., Kalapothakis E., Diniz C.R., Cordeiro M.N., Santos T.M., Prado M.A., Gomez M.V. Phoneutria nigriventer toxins block tityustoxin-induced calcium influx in synaptosomes. Neuroreport. 9:1998;1371-1373.
30. Miranda D.M., Romano-Silva M.A., Kalapothakis E., Diniz C.R., Cordeiro M.N., Moraes-Santos T., De Marco L., Prado M.A., Gomez M.V. Spider neurotoxins block the beta scorpion toxin-induced calcium uptake in rat brain cortical synaptosomes. Brain Res. Bull. 54:2001;533-536.
31. Norton R.S., Pallaghy P.K. The cystine knot structure of ion channel toxins and related polypetides. Toxicon. 36:1998;1573-1583.
32. Park C.S., Miller C. Mapping function to structure in a channel-blocking peptide: electrostatic mutants of charybdotoxin. Biochemistry. 31:1992;7749-7755.
33. + channles. Proc. Natl. Acad. Sci. USA. 88:1991;2046-2050.
34. Penaforte C.L., Prado V.F., Prado M.A., Romano-Silva M.A., Guimaraes P.E., DeMarco L., Gomez M.V., Kalapothakis E. Molecular cloning of cDNAs encoding insecticidal neurotoxic peptides from the spider Phoneutria nigriventer. Toxicon. 38:2000;1443-1449.
35. + channel pharmacology. Trends Pharmacol. Sci. 13:1992;359-365.
36. Prado M.A., Guatimosim C., Gomez M.V., Diniz C.R., Cordeiro M.N., Romano-Silva M.A. A novel tool for the investigation of glutamate release from rat cerebrocortical synaptosomes: the toxin Tx3-3 from the venom of the spider Phoneutria nigriventer. Biochem. J. 314:1996;145-150.
37. Rash L.D., Hodgson W.C. Pharmacology and biochemistry of spider venoms. Toxicon. 40:2002;225-254.
38. Reis H.J., Prado M.A., Kalapothakis E., Cordeiro M.N., Diniz C.R., De Marco L.A., Gomez M.V., Romano-Silva M.A. Inhibition of glutamate uptake by a polypeptide toxin (phoneutriatoxin 3-4) from the spider Phoneutria nigriventer. Biochem. J. 343:1999;413-418.
39. Reis H.J., Gomez M.V., Kalapothakis E., Diniz C.R., Cordeiro M.N., Prado M.A., Romano-Silva M.A. Inhibition of glutamate uptake by Tx3-4 is dependent on the redox state of cysteine residues. Neuroreport. 11:2000;2191-2194.
40. Rezende L. Jr., Cordeiro M.N., Oliveira E.B., Diniz C.R. Isolation of neurotoxic peptides from the venom of the armed spider Phoneutria nigriventer. Toxicon. 29:1991;1225-1233.
41. 2+ entry linked to rapid glutamate release: studies using the Phoneutria nigriventer toxin PhTX2 and potassium depolarization. Biochem. J. 296:1993;313-319.
42. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
43. + channels. J. Biol. Chem. 270:1995;25121-25126.
44. + permeability. J. Neurosci. 6:1996;1570-1574.
45. + channel blocker. Biochemistry. 32:1993;5692-5697.
46. Smith L.A., Olson M.A., Lafaye P.J., Dolly J.O. Cloning and expression of mamba toxins. Toxicon. 33:1995;459-474.
47. + channel structure from a complete functional map of the molecular surface of charybdotoxin. Biochemistry. 33:1994;443-450.
48. + channels. Neuron. 18:1997;675-682.
49. v1.5 gene expression. Biochemistry. 35:1996;14149-14156.
50. 3 clonal pituitary cells. Biochim. Biophys. Acta. 1265:1995;22-28.
51. Zhu S., Li W., Zeng X., Jiang D., Mao X., Liu H. Molecular cloning and sequencing of two short chain and two long chain K(+)channel-blocking peptides from the Chinese scorpion Buthus martensii Karsch. FEBS Lett. 457:1999;509-514.