A toxin from the spider Phoneutria nigriventer that blocks calcium channels coupled to exocytosis

British Journal of Pharmacology

Volumn 122, Issue 3, 1997, Pages 591-597

  Guatimosim, C ^a   Romano Silva, M A ^a   Cruz, J S ^a   Beirao P S L ^a   Kalapothakis, E ^a   Moraes Santos, T ^a   Cordeiro, M N ^b   Diniz, C R ^b   Gomez, M V ^a   Prado, M A M ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b FUNDAÇÃO EZEQUIEL DIAS   (Brazil)

Author keywords

agatoxin IVA; conotoxin GVIA; conotoxin MVIIC; Calcium channels; Exocytosis; FM1 43; Phoniatry toxin; Synaptic vesicles; Synaptosomes

Indexed keywords

CALCIUM CHANNEL; CALCIUM ION; FLUORESCENT DYE; OMEGA AGATOXIN IVA; OMEGA CONOTOXIN GVIA; SPIDER VENOM;

ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CONTROLLED STUDY; EXOCYTOSIS; FEMALE; MALE; MEMBRANE DEPOLARIZATION; NONHUMAN; PRIORITY JOURNAL; RAT; SYNAPSE VESICLE; SYNAPTOSOME;

ANIMALS; CALCIUM; CALCIUM CHANNEL BLOCKERS; CEREBRAL CORTEX; EXOCYTOSIS; FEMALE; MALE; NEUROPEPTIDES; NEUROTOXINS; OMEGA-AGATOXIN IVA; OMEGA-CONOTOXIN GVIA; PEPTIDES; POTASSIUM CHLORIDE; RATS; RATS, WISTAR; SPIDER VENOMS; SYNAPTIC VESICLES; SYNAPTOSOMES;

EID: 9844266792     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0701381     Document Type: Article

References (35)

1. BETZ, W.J., MAO, F. & BEWICK, G.S. (1992). Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals. J. Neurosci, 12, 363-375.
2. BETZ, W.J., MAO, F. & SMITH, C.B. (1996). Imaging exocytosis and endocytosis. Curr. Opinions Neurobiol., 6, 365-371.
3. CORDEIRO, M.N., FIGUEIREDO, S.G., VALENTIM, A.C., DINIZ, C.R., EICKSTEDT, V.R.D., GILROY, J. & RICHARDSON, M. (1993). Purification and amino acid sequences of six TX3 type neurotoxins from the venom of the Brazilian "armed" spider Phoneutria nigriventer (keys). Toxicon, 31, 35-42.
4. DUNKLEY, P.R., HEATH, J.W., HARRISON, S.M., JARVIE, P.E., GLENFIELD, P.J. & ROSTAS, J.A.P. (1988). A rapid Percoll gradient procedure for isolation of synaptosomes directly from a S1 fraction: homogeneity and morphology of subcellular fractions. Brain Res., 441, 59-71.
5. DUNLAP, K., LUEBKE, J.L. & TURNER, T.J. (1995). Exocytotic calcium channels in mammalian central neurons. Trends Neurosci., 18, 89-98.
6. GOMEZ, M.V. & DINIZ, C.R. (1966). Separation of toxic components from the Brazilian scorpion Tityus serrulatus venom. Mem. Inst. Butantan, 33, 899-902.
7. GOMEZ, M.V., ROMANO-SILVA, M.A. & PRADO, M.A.M. (1995). Effects of tityustoxin on central nervous system. J. Toxicol. (Toxin Rev.), 14, 437-456.
8. GRYKIEWICZ, G., POENIE, M. & TSIEN, R.Y. (1985). A new generation of calcium indicators with greatly improved fluorescence properties. J. Biol. Chem., 260, 3440-3450.
9. HENKEL, A.W., LÜBKE, J. & BETZ, W.J. (1996). KM1-43 ultrastructural localisation in and release from frog motor nerve terminals. Proc. Natl. Acad. Sci. U.S.A., 93, 1918-1923.
10. LÉVÊQUE, C., MARTIN-MOUTOT, N., SATO, K., KATO, R., TAKAHASHI, M. & SEAGAR, M.J. (1994). Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. J. Biol. Chem., 269, 6306-6312.
11. LLINÁS, R., SUGIMORI, M., HILMAN, D.E. & CHERKSEY, B. (1989). Blocking and isolation of a calcium channel from neurons in mammals and cephalopods utilising a toxin fraction (FTX) from funnel-web spider poison. Proc. Natl. Acad. Sci. U.S.A., 86, 1689-1693.
12. LUEBKE, J.I., DUNLAP, K. & TURNER, T.J. (1993). Multiple calcium channel types control glutamatergic synaptic transmission in the hippocampus. Neuron, 11, 895-902.
13. MARTIN-MOUTOT, N., CHARVIN, N., LÉVÊQUE, C., SATO, K., NISHIKI, T., KOZAKI, S., TAKAHASHI, M. & SEAGAR, M. (1996). Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. J. Biol. Chem., 271, 6567-6570.
14. MATTHEWS, G. (1996). Synaptic exocytosis and endocytosis: capacitance measurements. Curr. Opinion Neurobiol., 6, 358-364.
15. 2+-independent synaptic vesicle release. Neuron, 12, 1235-1244.
16. MILJANICH, C.P. & RAMACHANDRAN, J. (1995). Antagonists of neuronal calcium channels: structure, function and therapeutic implications. Ann. Rev. Pharmacol. Toxicol., 35, 707-734.
17. MINTZ, I.M., ADAMS, M.E. & BEAN, B.P. (1992a). P-type channels in rat central and peripheral neurons. Neuron, 9, 85-95.
18. MINTZ, I.M., VENEMA, V.J., SWIDEREK, K.M., LEE, T.D., BEAN, B.P. & ADAMS, M.E. (1992b). P-type calcium channels blocked by ω-Aga IVA. Nature, 355, 827-829.
19. OLIVERA, B.M., MILJANICH, G.P., RAMACTIANDRAN, J. & ADAMS, M.E. (1994). Calcium channel diversity and neurotransmitter release: The ω-conotoxins and ω-Agatoxins. Ann. Rev. Biochem., 63, 823-867.
20. PRADO, M.A.M., GUATIMOSIM, C., GOMEZ, M.V., DINTZ, C.R., CORDEIRO, M.N. & ROMANO-SILVA, M.A. (1996). A novel tool for the investigation of glutamate release from rat cerebrocortical synaptosomes: the toxin tx3-3 from the venom of the spider Phoncutria nigriventer. Biochem. J., 314, 145-150.
21. RANDALL, A. & TSIEN, R.W. (1995). Pharmacological dissection of multiple types of calcium channel currents in rat cerebellar neurons. J. Neurosci., 15, 2995-3012.
22. REUTER, H. & PORZIG, H. (1995). Localisation and functional significance of the sodium calcium exchanger in presynaptic boutons of hippocampal cells in culture. Neuron, 15, 1077-1084.
23. REUTER, H. (1996). Diversity and function of presynaptic calcium channels in the brain. Curr. Opinions Neurobiol., 6, 331-337.
24. ROMANO-SILVA, M.A., RIBEIRO-SANTOS, R., RIBEIRO, A.M., GOMEZ, M.V., DINIZ, C.R., CORDEIRO, M.N. & BRAMMER, M.J. (1993). Rat cortical synaptosomes have more than one mechanism for calcium entry linked to rapid glutamate release: studies using the Phoneutria nigriventer toxin PhTX2 and potassium depolarisation. Biochem. J., 294, 313-319.
25. SNUTCH, T.P., LEONARD, J.P., GILBERT, M.M., LESTER, H.A. & DAVIDSON, N. (1991). Rat brain expresses a heterogeneous family of calcium channels. Proc. Natl. Acad. Sci. U.S.A., 87, 3391-3395.
26. 1A calcium channel reflect similarities to neuronal Q- and P-type channels. Proc. Natl. Acad. Sci. U.S.A., 91, 10576-10580.
27. TURNER, T.J., ADAMS, M.E. & DUNLAP, K. (1992). Calcium channels coupled to glutamate release identified by ω-Aga IVA. Science, 258, 310-313.
28. TURNER, T.J., ADAMS, M.E. & DUNLAP, K. (1993). Multiple calcium channel types coexist to regulate synaptosomal neurotransmitter release. Proc. Natl. Acad. Sci. U.S.A., 90, 9518-9522.
29. TURNER, T.J. & DUNLAP, K. (1995). Pharmacological characterisation of presynaptic calcium channels using subsecond biochemical measurements of synaptosomal secretion. Neuropharmacohgy, 34, 1469-1478.
30. TURNER, T.J., LAMPE, R.A. & DUNLAP, K. (1995). Characterisation of presynaptic calcium channels with omega MVIIC and omega grammotoxina SIA: role for a resistant calcium channel type in neurosecretion. Mol. Pharmacol., 47, 348-353.
31. VARADI, G., MORI, Y., MIKALA, G. & SCHWARTZ, A. (1995). Molecular determinants of calcium channel function and drug action. Trends. Pharmacol. Sci., 16, 43-49.
32. 1A subunits of brain calcium channels. J. Neurosci., 15, 6403-6418.
33. WHEELER, D.B., RANDALL, A. & TSIEN, R.W. (1994). Roles of N-type and Q-type calcium channels in supporting hippocampal synaptic transmission. Science, 264, 107-111.
34. YOSHIDA, A., OHO, C., OMORI, A., KUWAHARA, R., ITO, T. & TAKAHASHI, M. (1992). HPC-1 is associated with synaptotagmin and ω-conotoxin receptor. J. Biol. Chem., 267, 24925-24928.
35. ZHANG, J.F., RANDALL, A.D., ELLINOR, P.T., HORNE, W.A., SATHER, W.A., TANABE, T., SCHWARZ, T.L. & TSIEN, R.W. (1993). Distinctive pharmacology and kinetics of cloned neuronal calcium channels and their possible counterparts in mammalian CNS neurons. Neuropharmacology, 32, 1075-1088.