Application of single-strand conformation polymorphism and denaturing gradient gel electrophoresis for fla sequence typing of Campylobacter jejuni

Journal of Microbiological Methods

Volumn 52, Issue 3, 2003, Pages 305-313

  Hein, Ingeborg ^a   Mach, Robert L ^b   Farnleitner, Andreas H ^b   Wagner, Martin ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Amplified fragment length polymorphism; Campylobacter coli; Campylobacter jejuni; Denaturing gradient gel electrophoresis; Pulsed field gel electrophoresis; Single strand conformation polymorphism

Indexed keywords

DNA FRAGMENT;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; DUCK; FLA GENE; FOWL; GEL ELECTROPHORESIS; GENE; GENE SEQUENCE; GENETIC POLYMORPHISM; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM;

BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; CAMPYLOBACTER JEJUNI; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLAGELLIN; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

ANAS; BACTERIA (MICROORGANISMS); CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI;

EID: 0037346788     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00183-5     Document Type: Article

References (37)

1. Bassam B.J., Caetano-Anollés G., Gresshoff P.M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196:1991;80-83.
2. Bennasar A., Guasp C., Tesar M., Lalucat J. Genetic relationships among Pseudomonas stutzeri strains based on molecular typing methods. J. Appl. Microbiol. 85:1998;643-656.
3. Charvalos, E., Peteinaki, E., Spyridaki, I., Manetas, S., Tselentis, Y., 1996. Detection of ciprofloxacin resistance mutations in Campylobacter jejuni gyrA by nonradioisotopic single-strand conformation polymorphism and direct DNA sequencing.
4. Dabert P., Fleurat-Lessard A., Mounier E., Delgenes J.P., Moletta R., Godon J.J. Monitoring of the microbial community of a sequencing batch reactor bioaugmented to improve its phosphorus removal capabilities. Water Sci. Technol. 43:2001;1-8.
5. DeBoer P., Duim B., Rigter A., Plas J. v.d., Jacobs-Reitsma W.F., Wagenaar J.A. Computer-assisted analysis and epidemiological value of genotyping methods for Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 38:2000;1940-1946.
6. Duim B., Wassenaar T.M., Rigter A., Wagenaar J. High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting. Appl. Environ. Microbiol. 65:1999;2369-2375.
7. Farnleitner A.H., Kreuzinger N., Kavka G.G., Grillenberger S., Rath J., Mach R.L. Comparative analysis of denaturing gradient gel electrophoresis (DGGE) and temporal temperature gradient electrophoresis (TTGE) in separating of Escherichia coli uidA amplicons differing in single base substitutions. Lett. Appl. Microbiol. 30:2000;427-431.
8. Farnleitner A.H., Kreuzinger N., Kavka G.G., Grillenberger S., Rath J., Mach R.L. Simultaneous detection and differentiation of Escherichia coli populations from environmental freshwaters by means of sequence variations in a fragment of the β-D-glucuronidase gene. Appl. Environ. Microbiol. 66:2000;1340-1346.
9. Farnleitner A.H., Reischer G., Zibuschka F., Lindner G., Mach R.L. Comparison and differentiation of heterotrophic plate count communities in raw and drinking water by eubacterial 16S-rDNA amplicon profiling. Vom Wasser. 97:2001;167-180.
10. Gibson J.R., Sutherland K., Owen R.J. Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni. Lett. Appl. Microbiol. 19:1994;357-358.
11. Hänninen M.L., Hakkinen M., Rautelin H. Stability of related human and chicken Campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis. Appl. Environ. Microbiol. 65:1999;2272-2275.
12. Harrington C.S., Thomson Carter F.M., Carter P.E. Evidence for recombination in the flagellin locus of Campylobacter jejuni: implications for the flagellin gene typing scheme. J. Clin. Microbiol. 35:1997;2386-2392.
13. Hunter P.R., Gaston M.A. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26:1988;2465-2466.
14. Lan R., Reeves P.R. Unique adaptor design for AFLP fingerprinting. Biotechniques. 29:2000;745-750.
15. Lehner A., Loncarevic S., Wagner M., Kreike J., Brandl E. A rapid differentiation of Listeria monocytogenes by use of PCR-SSCP in the listeriolysin O (hlyA) locus. J. Microbiol. Methods. 34:1999;165-171.
16. Lehner A., Schneck C., Feierl G., Pless P., Deutz A., Brandl E., Wagner M. Epidemiologic application of pulsed-field gel electrophoresis to an outbreak of Campylobacter jejuni in an Austrian youth centre. Epidemiol. Infect. 125:2000;13-16.
17. Lucey B., Feurer C., Greer P., Moloney P., Cryan B., Fanning S. Antimicrobial resistance profiling and DNA amplification fingerprinting (DAF) of thermophilic Campylobacter spp. in human, poultry and porcine samples from the Cork region of Ireland. J. Appl. Microbiol. 89:2000;727-734.
18. Manzano M., Cocolin L., Pipan C., Falasca E., Botta G.A., Cantoni C., Comi G. Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as a tool to detect different serogroups. Mol. Cell. Probes. 11:1997;459-462.
19. Moore J.E., Stanley T., Smithson R., O'Malley H., Murphy P.G. Outbreak of Campylobacter food-poisoning in Northern Ireland. Clin. Microbiol. Infect. Dis. 6:2000;385-398.
20. Moore J., Curran M., Wareing D., Fox A., Boyd N., Glynn G., Millar B., Daly G., Murphy P. Investigation of infection with Campylobacter jejuni in a man with hypogammaglobulinaemia using PCR-single-stranded conformational polymorphism (PCR-SSCP) typing. Int. J. Med. Microbiol. 291:2001;21-25.
21. Muyzer G., Smalla K. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoek. 73:1998;127-141.
22. Myers R.M., Fischer S.G., Lerman L.S., Maniatis T. Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res. 13:1985;3131-3145.
23. Nielsen E.M., Engberg J., Fussing V., Petersen L., Brogren C.H., On S.L.W. Evaluation of phenotypic and genotypic methods for subtyping Campylobacter jejuni isolates from humans, poultry, and cattle. J. Clin. Microbiol. 38:2000;3800-3810.
24. Nishimura M., Nukina M., Yuan J.M., Shen B.Q., Ma J.J., Ohta M., Saida T., Uchiyama T. PCR-based restriction fragment length polymorphism (RFLP) analysis and serotyping of Campylobacter jejuni isolates from diarrheic patients in China and Japan. FEMS Microbiol. Lett. 142:1996;133-138.
25. Olsen S.J., Hansen G.R., Bartlett L., Fitzgerald C., Sonder A., Manjrekar R., Riggs T., Kim J., Flahart R., Pezzino G., Swerdlow D.L. An outbreak of Campylobacter jejuni infections associated with food handler contamination: the use of pulsed-field gel electrophoresis. J. Infect. Dis. 183:2001;164-167.
26. On S.L.W. In vitro genotypic variation of Campylobacter coli documented by pulsed-field gel electrophoresis DNA profiling: implications for epidemiological studies. FEMS Microbiol. Lett. 165:1998;341-346.
27. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. U. S. A. 86:1989;2766-2770.
28. Petersen L., Newell D.G. The ability of fla-typing schemes to discriminate between strains of Campylobacter jejuni. J. Appl. Microbiol. 91:2001;217-224.
29. Petersen L., On S.L.W. Efficacy of flagellin gene typing for epidemiological studies of Campylobacter jejuni in poultry estimated by comparison with macrorestriction profiling. Lett. Appl. Microbiol. 31:2000;14-19.
30. Sheffield V.C., Cox D.R., Lerman L.S., Myers R.M. Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc. Natl. Acad. Sci. U. S. A. 86:1989;232-236.
31. Simpson J.M., McCracken V.J., White B.A., Gaskins H.R., Mackie R.I. Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota. J. Microbiol. Methods. 36:1999;167-179.
32. Studer E., Domke M., Wegmüller B., Lüthy J., Schmid S., Candrian U. RFLP and sequence analysis of Campylobacter jejuni and Campylobacter coli PCR products amplified directly from environmental samples. Lebensm.-Wiss. Technol. 31:1998;537-545.
33. Tauxe R.V. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. Nachamkin I., Blaser M.J., Tompkins L.S. Campylobacter jejuni: Current Status and Future Trends. 1992;9-19 American Society for Microbiology, Washington, DC.
34. Wassenaar T.M., Newell D.G. Genotyping of Campylobacter spp. Appl. Envrion. Microbiol. 66:2000;1-9.
35. Wassenaar T.M., Geilhausen B., Newell D.G. Evidence of genomic instability in Campylobacter jejuni isolated from poultry. Appl. Environ. Microbiol. 64:1998;1816-1821.
36. Wegmüller B., Lüthy J., Candrian U. Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products. Appl. Environ. Microbiol. 59:1993;2161-2165.
37. Widjojoatmodjo M.N., Fluit A.C., Verhoef J. Rapid identification of bacteria by PCR-single-strand conformation polymorphism. J. Clin. Microbiol. 32:1994;3002-3007.