Desensitization of Gs-coupled receptor signaling by constitutively active mutants of the human lutropin/choriogonadotropin receptor

Journal of Clinical Endocrinology and Metabolism

Volumn 88, Issue 3, 2003, Pages 1194-1204

  Shinozaki, Hiromitsu ^a   Butnev, Viktor ^a   Tao, Ya Xiong ^a   Ang, Kok Long ^b   Conti, Marco ^b   Segaloff, Deborah L ^a  

^a UNIVERSITY OF IOWA   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; CHORIONIC GONADOTROPIN RECEPTOR; CYCLIC AMP; FORSKOLIN; G PROTEIN COUPLED RECEPTOR; GS PROTEIN COUPLED RECEPTOR; ISOPRENALINE; LUTEINIZING HORMONE RECEPTOR; LUTEINIZING HORMONE RECEPTOR L457R; MUTANT PROTEIN; PHOSPHODIESTERASE; PHOSPHODIESTERASE 4D3; PHOSPHODIESTERASE INHIBITOR; ROLIPRAM; UNCLASSIFIED DRUG;

ARTICLE; ATTENUATION; CELL ISOLATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG BINDING; DRUG MECHANISM; ENZYME ACTIVATION; GENE ACTIVATION; GENE MUTATION; GENETIC TRANSFECTION; GONADOTROPIN RELEASE; HETEROZYGOSITY; HORMONAL REGULATION; HORMONE RECEPTOR INTERACTION; HUMAN; HUMAN CELL; PRECOCIOUS PUBERTY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RECEPTOR BINDING; RECEPTOR DOWN REGULATION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; TESTOTOXICOSIS; WILD TYPE;

3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; CELLS, CULTURED; CHORIONIC GONADOTROPIN; CYCLIC AMP; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; HUMANS; MUTATION; RECEPTORS, LH;

EID: 0037341869     PISSN: 0021972X     EISSN: None     Source Type: Journal    
DOI: 10.1210/jc.2002-021051     Document Type: Article

References (41)

1. Rajagopalan-Gupta RM, Lamm ML, Mukherjee S, Rasenick MM, Hunzicker-Dunn M 1998 Luteinizing hormone/choriogonadotropin receptor-mediated activation of heterotrimeric guanine nucleotide binding proteins in ovarian follicular membranes. Endocrinology 139:4547-4555
2. Rajagopalan-Gupta RM, Rasenick MM, Hunzicker-Dunn M 1997 Luteinizing hormone/choriogonadotropin-dependent, cholera toxin-catalyzed adenosine 5′-diphosphate (ADP)-ribosylation of the long and short forms of Gsα and pertussis toxin-catalyzed ADP-ribosylation of Giα. Mol Endocrinol 11:538 -549
3. +2 mobilization. J Biol Chem 267:4479-4488
4. Wang Z, Hipkin RW, Ascoli M 1996 Progressive cytoplasmic tail truncations of the lutropin-choriogonadotropin receptor prevent agonist- or phorbol ester-induced phosphorylation, impair agonist- or phorbol ester-induced desensitization, and enhance agonist-induced receptor down-regulation. Mol Endocrinol 10:748-758
5. Herrlich A, Kuhn B, Grosse R, Schmid A, Schultz G, Gudermann T 1996 Involvement of Gs and Gi proteins in dual coupling of the luteinizing hormone receptor to adenylyl cyclase and phospholipase C. J Biol Chem 271:16764-16772
6. Latronico A, Segaloff D 1999 Naturally occurring mutations of the luteinizing-hormone receptor: lessons learned about reproductive physiology and G protein-coupled receptors. Am J Hum Genet 65:949-958
7. Themmen APN, Huhtaniemi IT 2000 Mutations of gonadotropins and gonadotropin receptors; elucidating the physiology and pathophysiology of pituitary-gonadal function. Endocr Rev 21:551-583
8. Latronico AC, Abell AN, Arnhold IJP, Liu X, Lins TSS, Brito VN, Billerbeck AE, Segaloff DL, Mendonca BB 1998 A unique constitutively activating mutation in the third transmembrane helix of the luteinizing hormone receptor causes sporadic male gonadotropin independent precocious puberty. J Clin Endocrinol Metab 83:2435-2440
9. Shinozaki H, Fanelli F, Liu X, Jaquette J, Nakamura K, Segaloff DL 2001 Pleiotropic effects of substitutions of a highly conserved leucine in transmembrane helix III of the human lutropin/choriogonadotropin receptor with respect to constitutive activation and hormone responsiveness. Mol Endocrinol 15:972-984
10. Ascoli M, Puett D 1978 Gonadotropin binding and stimulation of steroido-genesis in Leydig tumor cells. Proc Natl Acad Sci USA 75:99-102
11. Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR 1989 Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59
12. Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR 1989 Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68
13. Tao Y-X, Abell AN, Liu X, Nakamura K, Segaloff DL 2000 Constitutive activation of G protein coupled receptors as a result of selective substitution of a conserved leucine residue in transmembrane helix III. Mol Endocrinol 14:1272-1282
14. Chen C, Okayama H 1987 High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7:2745-2752
15. Nakamura K, Lazari MF, Li S, Korgaonkar C, Ascoli M 1999 Role of the rate of internalization of the agonist-receptor complex on the agonist-induced down-regulation of the lutropin/choriogonadotropin receptor. Mol Endocrinol 13:1295-1304
16. Wiley H, Cunningham D 1982 The endocytotic rate constant. A cellular parameter for quantitating receptor-mediated endocytosis. J Biol Chem 257:4222-4229
17. Ascoli M 1982 Internalization and degradation of receptor-bound human choriogonadotropin in Leydig tumor cells. Fate of the hormone subunits. J Biol Chem 257:13306-13311
18. Oki N, Takahashi SI, Hidaka H, Conti M 2000 Short term feedback regulation of cAMP in FRTL-5 thyroid cells. Role of PDE4D3 phosphodiesterase activation. J Biol Chem 275:10831-10837
19. Min L, Ascoli M 2000 Effect of activating and inactivating mutations on the phosphorylation and trafficking of the human lutropin/choriogonadotropin receptor. Mol Endocrinol 14:1797-1810
20. Hipkin RW, Sanchez-Yague J, Ascoli M 1992 Identification and characterization of an LH/CG receptor precursor in a human kidney cell line stably transfected with the rat luteal LH/CG receptor cDNA. Mol Endocrinol 6:2210-2218
21. Sette C, Conti M 1996 Phosphorylation and activation of a cAMP-specific phosphodiesterase by the cAMP-dependent protein kinase. Involvement of serine 54 in the enzyme activation. J Biol Chem 271:16526-16534
22. Kosugi S, Van Dop C, Geffner ME, Rabl W, Carel JC, Chaussain JL, Mori T, Merendino Jr JJ, Shenker A 1995 Characterization of heterogeneous mutations causing constitutive activation of the luteinizing hormone receptor in familial male precocious puberty. Hum Mol Gen 4:183-188
23. Kawate N, Kletter GB, Wilson BE, Netzloff ML, Menon KMJ 1995 Identification of constitutively activating mutation of the luteinising hormone receptor in a family with male limited gonadotrophin independent precocious puberty (testotoxicosis). J Med Genet 32:553-554
24. Laue L, Chan W-C, Hsueh A, Kudo M, Hsu SY, Wu S, Blomberg L, Cutler GB 1995 Genetic heterogeneity of constitutively activating mutations of the human luteinizing hormone receptor in familial male-limited precocious puberty, Proc Natl Acad Sci USA 92:1906-1910
25. Muller J, Gondos B, Kosugi S, Mori T, Shenker A 1998 Severe testotoxicosis phenotype associated with Asp578→Tyr mutation of the lutrophin/choriogonadotrophin receptor gene. J Med Genet 35:340-341
26. Conti M 2000 Phosphodiesterases and cyclic nucleotide signaling in endocrine cells. Mol Endocrinol 14:1317-1327
27. Grant PG, Mannarino AF, Colman RW 1988 cAMP-mediated phosphorylation of the low-Km cAMP phosphodiesterase markedly stimulates its catalytic activity. Proc Natl Acad Sci USA 85:9071-9075
28. Gettys TW, Vine AJ, Simonds MF, Corbin JD 1988 Activation of the particulate low Km phosphodiesterase of adipocytes by addition of cAMP-dependent protein kinase J Biol Chem 263:10359-10363
29. Sette C, Iona S, Conti M 1994 The short-term activation of a rolipram-sensitive, cAMP-specific phosphodiesterase by thyroid-stimulating hormone in thyroid FRTL-5 cells is mediated by a cAMP-dependent phosphorylation. J Biol Chem 269:9245-9252
30. MacKenzie SJ, Baillie GS, McPhee I, Bolger GB, Houslay MD 2000 ERK2 mitogen-activated protein kinase binding, phosphorylation, and regulation of the PDE4D cAMP-specific phosphodiesterases. The involvement of COOH-terminal docking sites and NH2-terminal UCR regions. J Biol Chem 275:16609-16617
31. Yarwood SJ, Steele MR, Scotland G, Houslay MD, Bolger GB 1999 The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 274:14909-14917
32. Swinnen JV, Joseph DR, Conti M 1989 The mRNA encoding a high-affinity cAMP phosphodiesterase is regulated by hormones and cAMP. Proc Natl Acad Sci USA 86:8197-8201
33. Tasken KA, Collas P, Kemmner WA, Witczak O, Conti M, Tasken K 2001 Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area. J Biol Chem 276:21999-22002
34. Dodge KL, Khouangsathiene S, Kapiloff MS, Mouton R, Hill EV, Houslay MD, Langeberg LK, Scott JD 2001 mAKAP assembles a protein kinase A/PDE4 phosphodiesterase cAMP signaling module. EMBO J 20:1921-1930
35. Persani L, Lania A, Alberti L, Romoli R, Mantovani G, Filetti S, Spada A, Conti M 2000 Induction of specific phosphodiesterase isoforms by constitutive activation of the cAMP pathway in autonomous thyroid adenomas. J Clin Endocrinol Metab 85:2872-2878
36. Lania A, Persani L, Ballare E, Mantovani S, Losa M, Spada A 1998 Constitutively active Gsα is associated with an increased phosphodiesterase activity in human growth hormone-secreting adenomas. J Clin Endocrinol Metab 83:1624-1628
37. Huang X-P, Song X, Wang H-Y, Malbon CC 2002 Targeted expression of activated Q227L Gα s in vivo. Am J Physiol Cell Physiol 283:C386-C395
38. Rosenthal SM, Grumbach MM, Kaplan SL 1983 Gonadotropin-independent familial sexual precocity with premature Leydig and germinal cell maturation (familial testotoxicosis): effects of a potent luteinizing hormone-releasing factor agonist and medroxyprogesterone acetate therapy in four cases. J Clin Endocrinol Metab 57:571-579
39. Harwood JP, Conti M, Conn PM, Dufau ML, Catt KJ 1978 Receptor regulation and target cell responses: studies in the ovarian luteal cell. Mol Cell Endocrinol 11:121-135
40. Catt KJ, Harwood JP, Clayton RN, Davies TF, Chan V, Katikineni M, Nozu K, Dufau ML 1980 Regulation of peptide hormone receptors and gonadal steroidogenesis. Recent Prog Horm Res 36:557-622
41. Hirakawa T, Galet C, Ascoli M 2002 MA-10 cells transfected with the human lutropin/choriogonadotropm receptor (hLHR): a novel experimental paradigm to study the functional properties of the hLHR. Endocrinology 143:1026-1035