The ribonuclease H activity of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2 is modulated by residue 294 of the small subunit

Nucleic Acids Research

Volumn 31, Issue 5, 2003, Pages 1481-1487

  Sevilya, Z ^b   Loya, S ^b   Adir, N ^a   Hizi, A ^b  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMINE; PROLINE; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE;

AMINO ACID SUBSTITUTION; BINDING AFFINITY; CONTROLLED STUDY; DNA RNA HYBRIDIZATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE; ENZYME SUBUNIT; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; REVIEW; VIRUS MUTANT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; DNA-DIRECTED DNA POLYMERASE; GLUTAMINE; HIV-1; HIV-1 REVERSE TRANSCRIPTASE; HIV-2; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS; RIBONUCLEASE H, CALF THYMUS; RNA-DIRECTED DNA POLYMERASE; SEQUENCE HOMOLOGY, AMINO ACID;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2; RNA VIRUSES;

EID: 0037338420     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg235     Document Type: Review

References (26)

1. Skalka,A.M. and Goff,S.P. (1993) Reverse Transcriptase. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Goff,S.P. (1990) Retroviral reverse transcriptases: synthesis, structure and function. J. AIDS, 3, 817-831.
3. Coffin,J.M., Hughes,S.H. and Varmus,H.E. (1997) Retroviruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
4. Veronese,F.D., Copeland,T.D., Devico,A.L., Rahman,R. and Gallo,R.C. (1986) Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. Science, 231, 1291-1298.
5. DeVico,A.L., Copeland,T.D., Veronese,F.D., Oroszlan,S., Gallo,R.C. and Sarnagadharan,M.G. (1989) Purification and partial characterization of human immunodeficiency virus type-2 reverse transcriptase. AIDS Res. Hum. Retroviruses, 5, 51-60.
6. Kohlstaedt,L.A., Wang,J., Friedman,J.M., Rice,P.A. and Steitz,T.A. (1992) Crystal structure at 3.5 Å resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science, 256, 1783-1790.
7. Jacobo-Molina,A., Ding,J., Nami,R.G., Clark,A.D.J., Lu,X., Tanfillo,C., Williams,R.L., Kamer,G., Ferris,A.L., Clark,P. et al. (1990) Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 Å resolution shows bent DNA. Proc. Natl Acad. Sci. USA, 90, 6230-6234.
8. Huang,H., Chopra,R., Verdine,G.L. and Harrison,S.C. (1998) Structure of a covalently-trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. Science, 282, 1669-1675.
9. Ding,J., Das,K., Hsiou,Y., Sarafianos,S.G., Clark,A.G., Jacobo-Molina,A., Tantillo,C., Hughes,S.H. and Arnold,E. (1998) Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double stranded DNA template-primer and an antibody Fab fragment at 2.8 Å resolution. J. Mol. Biol., 284, 1095-1111.
10. Ren,J., Bird,L.E., Chamberlain,P.P., Stewart-Jones,G.B., Stuart,D.I. and Stammers,D.K. (2002) Structure of HIV-2 reverse transcriptase at 2.35-Å resolution and the mechanism of resistance to non-nucleoside inhibitors. Proc. Natl Acad. Sci. USA, 99, 14410-14415.
11. Sawaya,M.R., Pelletier,H., Kumar,A., Wilson,S.H. and Kraut,J. (1994) Crystal structure of rat DNA polymerase β: evidence for a common polymerase mechanism. Science, 264, 1930-1935.
12. Ollis,D.L., Brick,P., Hamlin,R., Xunong,N.G. and Steitz,T.A. (1985) Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP. Nature, 313, 762-766.
13. Li,Y., Korolev,S. and Waksman K. (1998) Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation. EMBO J., 17, 7514-7525.
14. Jeruzalmi,D. and Steitz,T.A. (1998) Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme. EMBO J., 17, 4101-4113.
15. Hansen,J.L., Long,A.M. and Schultz,S.C. (1997) Structure of the RNA-dependent RNA polymerase of poliovirus. Structure, 5, 1109-1122.
16. Georgiadis,M.M., Jessen,S.M., Ogata,C.M., Telesnitsky,A., Goff,S.P. and Hendrickson,W.A. (1995) Mechanistic implications from the structure of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase. Structure, 3, 879-892.
17. Shaharabany,M. and Hizi,A. (1992) The catalytic functions of chimeric reverse transcriptases of human immunodeficiency viruses type 1 and type 2. J. Biol. Chem., 267, 3647-3678.
18. Hizi,A., Tal,R., Shaharabany,M. and Loya,S. (1991) Catalytic properties of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2. J. Biol. Chem., 266, 6230-6239.
19. Hizi,A., Tal,R. and Hughes,S.H. (1991) Mutational analysis of the DNA polymerase and ribonuclease H activities of human immunodeficiency virus type 2 reverse transcriptase expressed in Escherichia coli. Virology, 180, 339-346.
20. Sevilya,Z., Loya,S., Hughes,S.H. and Hizi,A. (2001) The ribonuclease H activity of the reverse transcriptase of human immunodeficiency viruses type 1 and type 2 is affected by the thumb subdomain of the small subunits. J. Mol. Biol., 341, 957-971.
21. Hizi,A., McGill,C. and Hughes,S.H. (1988) Expression of soluble enzymatically active human immunodeficiency virus reverse transcriptase in Escherichia coli and analysis of mutants Proc. Natl Acad. Sci. USA, 85, 1218-1222.
22. Boyer,P.L., Ferris,A.L. and Hughes,S.H. (1992) Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1. J. Virol., 66, 1031-1039.
23. Fan,N., Rank,K.B., Poppe,S.M., Tarpley,W.G. and Sharma,S.K. (1996) Characterization of the p68/p58 heterodimer of human immunodeficiency virus type-2 reverse transcriptase. Biochemistry, 35, 1911-1917.
24. Madrid,M., Lukin,J.A., Madura,J.D., Ding,J. and Arnold,E. (2001) Molecular dynamics of HIV-1 reverse transcriptase indicates increased flexibility upon DNA binding. Proteins, 45, 176-182.
25. Sarafianos,S.G., Das,K., Tantillo,C., Clark,A.D.,Jr, Ding,J., Whitcomb,J., Boyer,P.L., Hughes,S.H. and Arnold,E. (2001) Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA. EMBO J., 20, 1449-1461.
26. Raush,J.W., Lener,D., Miller,J.T., Julias,J.G., Hughes,S.H. and Le Grice,S.F.J. (2002) Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity. Biochemistry, 41, 4856-4865.