Effects of β-adrenoceptor stimulation on endothelial nitric-oxide synthase phosphorylation of human umbilical vein endothelial cells

Acta Pharmacologica Sinica

Volumn 24, Issue 3, 2003, Pages 219-224

  Yao, Kang ^a   Xu, Biao ^a   Liu, Ya Ping ^a   Ferro, Albert ^b  

^a AFFILIATED ZHONGDA HOSPITAL OF SOUTHEAST UNIVERSITY   (China)

^b ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

Beta adrenergic receptors; Isoproterenol; Nitric oxide synthase; Umbilical veins; Vascular endothelium; Western blotting

Indexed keywords

3 ISOPROPYLAMINO 1 (7 METHYL 4 INDANYLOXY) 2 BUTANOL; 5 [2 [[2 HYDROXY 3 [4 (1 METHYL 4 TRIFLUOROMETHYL 2 IMIDAZOLYL)PHENOXY]PROPYL]AMINO]ETHOXY]SALICYLAMIDE; ARGININE; BETA 1 ADRENERGIC RECEPTOR; BETA 1 ADRENERGIC RECEPTOR BLOCKING AGENT; BETA 2 ADRENERGIC RECEPTOR; BETA 2 ADRENERGIC RECEPTOR BLOCKING AGENT; CITRULLINE; HEAT SHOCK PROTEIN 90; ISOPRENALINE; NITRIC OXIDE SYNTHASE; PHOSPHOSERINE; SERINE;

ARTICLE; BETA ADRENERGIC STIMULATION; CONTROLLED STUDY; DENSITOMETRY; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; UMBILICAL VEIN; VASCULAR ENDOTHELIUM; WESTERN BLOTTING;

ADRENERGIC BETA-AGONISTS; ADRENERGIC BETA-ANTAGONISTS; CELLS, CULTURED; ENDOTHELIAL CELLS; HUMANS; ISOPROTERENOL; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE III; PHOSPHORYLATION; PROPANOLAMINES; UMBILICAL VEINS;

EID: 0037337878     PISSN: 16714083     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Graves J, Poston L. Beta-adrenoceptor agonist mediated relaxation of rat isolated resistance arteries: a role for the endothelium and nitric oxide. Br J Pharmacol 1993; 108: 631-7.
2. Priest RM, Hucks D, Ward JPT. Noradrenaline, beta-adrenoceptor mediated vasorelaxation and nitric oxide in large and small pulmonary arteries of rat. Br J Pharmacol 1997; 122: 1375-81.
3. Ferro A, Queen LR, Priest RM, Xu B, Ritter JM, Poston L, Ward JPT. Activation of endothelial nitric oxide synthase by β2-adrenoceptors in human umbilical vein. Br J Pharmacol 1999; 126: 1872-80.
4. Xu B, Li J, Gao L, Ferro A. Nitric oxide-dependent vasodilation of rabbit femoral artery by β2-adrenergic stimulation or cyclic AMP elevation in vivo. Br J Pharmacol 2000; 129: 969-74.
5. Busse R, Fleming I. Regulation and functional consequences of endothelial nitric oxide formation. Ann Med 1995; 27: 331-40.
6. Kuchan MJ, Frangos JA. Role of calcium and calmodulin in flow-induced nitric oxide production in endothelial cells. Am J Physiol 1994; 266: C628-36.
7. Ayajiki K, Kindermann M, Hecker M, Fleming I, Busse R. Intracellular pH and tyrosine phosphorylation but not calcium determine shear stress-induced nitric oxide production in native endothelial cells. Circ Res 1996; 78: 750-8.
8. 2+, mobilization. Circ Res 1997; 81: 885-92.
9. Jaffe EA, Nachman RL, Becker CG, Minick RC. Culture of human endothelial cells derived from umbilical veins. J Clin Invest 1973; 52: 2745-56.
10. Dawss M, Chowienczy PJ, Ritter JM. Effect of inhibition of the L-arginine/nitric oxide pathway on vasodilation caused by β-adrenergic agonists in human forearm. Circulation 1997; 95: 2293-98.
11. Drexler H, Hayoz D, Munzel T. Endothelial function in chronic congestive heart failure. Am J Cardiol 1992; 69: 1596-601.
12. Larosa G, Forster C. Coronary beta-adrenoceptor function is modified by the endothelium in heart failure. J Vasc Res 1996; 33: 62-70.
13. Busse R, Mulsch A. Calcium-dependent nitric oxide synthasis in endothelial cytosol is mediated by calmodulin. FEBS Lett 1990; 265: 133-6.
14. Rosenkranz-WP, Sessa WC, Milstien S, Kanfman S, Watson CA, Pober JS. Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells: elevations in tetrahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity. J Clin Invest 1994; 93: 2236-43.
15. Fulton D, Gratton JR, McCabe TJ, Fontana J, Fujio Y, Walsh K. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 1999; 399: 597-601.
16. Dimmeler S, Fleming I, Fisslthaler B, Hermann C, Busse R, Zeiher AM. Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature 1999; 399: 601-5.
17. McCabe TJ, Fulton D, Roman LJ. Enhanced electron flux and reduced calmodulin dissociation may explain calcium-independent eNOS activation by phosphorylation. J Biol Chem 2000; 275: 6123-28.
18. Garcia-Cardena G, Fan R, Shah V, Sorrentino R, Cirino G, Sessa WC, et al. Dynamic activation of endothelial nitric oxide synthase by Hsp90. Nature 1998; 292: 821-4.
19. Shan V, Wiest R, Garcia-Cardena G, Cadelina G, Groszmann RJ, Sessa WC. HSP90 regulation of endothelial nitric oxide synthase contributes to vascular control in portal hypertension. Am J Physiol 1999; 27: G463-8.