Intracellular pH and tyrosine phosphorylation but not calcium determine shear stress-induced nitric oxide production in native endothelial cells

Circulation Research

Volumn 78, Issue 5, 1996, Pages 750-758

  Ayajiki, Kazuhide ^a   Kindermann, Markus ^a   Hecker, Markus ^a   Fleming, Ingrid ^a   Busse, Rudi ^a  

^a UNIVERSITY HOSPITAL FRANKFURT   (Germany)

Author keywords

mechanotransduction in endothelial cells; NO synthase; pH(i); protein kinase C; tyrosine kinase

Indexed keywords

(3 METHYLSULFONYL 4 PIPERIDINOBENZOYL)GUANIDINE METHANESULFONATE; 2 [1 (3 AMIDINOTHIOPROPYL) 1H INDOL 3 YL] 3 (1 METHYL 1H INDOL 3 YL)MALEIMIDE; CALPHOSTIN C; PROTEIN KINASE C; PROTEIN TYROSINE KINASE; STAUROSPORINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD VESSEL TONE; CALCIUM HOMEOSTASIS; CELL PH; CONTROLLED STUDY; CYTOSKELETON; ENDOTHELIUM CELL; ENZYME INHIBITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RABBIT; SHEAR STRESS; SIGNAL TRANSDUCTION; VASOCONSTRICTION;

EID: 0029914567     PISSN: 00097330     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.RES.78.5.750     Document Type: Article

References (37)

1. Bevan JA, Kaley G, Rubanyi GM. Flow-Dependent Regulation of Vascular Function. New York, NY: Oxford University Press; 1995.
2. Griffith TM, Edwards DH. Myogenic autoregulation of flow may be inversely related to endothelium-derived relaxing factor activity. Am J Physiol. 1990;258:H1171-H1180.
3. Pohl U, Herlan K, Huang A, Bassenge E. EDRF-mediated shear-induced dilation opposes myogenic vasoconstriction in small rabbit arteries. Am J Physiol. 1991;261:H2016-H2023.
4. Busse R, Mülsch A, Fleming I, Hecker M. Mechanisms of nitric oxide release from the vascular endothelium. Circulation. 1993; 87(suppl V):V-18-V-25.
5. Langille BL, Graham JJK, Kim D, Gotlieb AI. Dynamics of shear-induced redistribution of F-actin in endothelial cells in vivo. Arterioscler Thromb. 1991;11:1814-1820.
6. Franke R-P, Grafe M, Schnittler H, Seiffge D, Mittmayer C, Drenckhahn D. Induction of human vascular endothelial stress fibres by fluid shear stress. Nature. 1984;307:648-649.
7. Wechezak AR, Viggers RF, Sauvage LR. Fibronectin and F-actin redistribution in cultured endothelial cells exposed to shear stress. Lab Invest. 1985;53:639-647.
8. Davies PF, Tripathi SC. Mechanical stress mechanisms and the cell: an endothelial paradigm. Circ Res. 1993;72:239-245.
9. Morita T, Kurihara H, Maemura K, Yoshizumi M, Yazaki Y. Disruption of cytoskeletal structures mediates shear stress-induced endothelin-1 gene expression in cultured porcine aortic endothelial cells. J Clin Invest. 1993;92:1706-1712.
10. Davies PF, Barbee KA. Endothelial cell surface imaging: insights into hemodynamic force transduction. News Physiol Sci. 1994;9: 153-157.
11. Bhagyalakshmi A, Berthiaume F, Reich KM, Frangos JA. Fluid shear stress stimulates membrane phospholipid metabolism in cultured human endothelial cells. J Vasc Res. 1992;29:443-449.
12. Nollert MU, Eskin SG, McIntyre LV. Shear stress increases inositol trisphosphate levels in human endothelial cells. Biochem Biophys Res Commun. 1990;170:281-287.
13. Prasad ARS, Logan SA, Nerem RM, Schwartz CJ, Sprague EA. Flow-related responses of intracellular inositol phosphate levels in cultured aortic endothelial cells. Circ Res. 1993;72:827-836.
14. Rubanyi GM, Romero JC, Vanhoutte PM. Flow-induced release of endothelium-derived relaxing factor. Am J Physiol. 1986;250: H1145-H1149.
15. Pohl U, Holtz J, Busse R, Bassenge E. Critical role of endothelium in the vasodilator response to increased flow in vivo. Hypertension. 1986;8:37-44.
16. Ohno M, Gibbons GH, Dzau VJ, Cooke JP. Shear stress elevates endothelial cGMP: role of a potassium channel and G protein coupling. Circulation. 1993;88:193-197.
17. Ranjan V, Diamond SL. Fluid shear stress induces synthesis and nuclear localization of c-fos in cultured human endothelial cells. Biochem Biophys Res Commun. 1993;196:79-84.
18. Lan Q, Mercurius KO, Davies P. Stimulation of transcription factors NFκB and AP-1 in endothelial cells subjected to shear stress. Biochem Biophys Res Commun. 1994;201:950-956.
19. Falcone JC, Kuo L, Meininger GA. Endothelial cell calcium increases during flow-induced dilation in isolated arteries. Am J Physiol. 1993;264:H653-H659.
20. Shen J, Luscinskas FW, Connolly A, Dewey CF Jr, Gimbrone MA Jr. Fluid shear stress modulates cytosolic free calcium in vascular endothelial cells. Am J Physiol. 1992;262:C384-C390.
21. Schwarz G, Droogmans G, Nilius B. Shear stress induced membrane currents and calcium transients in human vascular endothelial cells. Pflugers Arch. 1992;421:394-396.
22. Hecker M, Mülsch A, Bassenge E, Busse R. Vasoconstriction and increased flow: two principal mechanisms of shear stress-dependent endothelial autacoid release. Am J Physiol. 1993;265:H828-H833.
23. Macarthur H, Hecker M, Busse R, Vane JR. Selective inhibition of agonist-induced but not shear stress-dependent release of endothelial autacoids by thapsigargin. Br J Pharmacol. 1993;108:100-105.
24. Busse R, Lamontagne D. Endothelium-derived bradykinin is responsible for the increase in calcium produced by angiotensin-converting enzyme inhibitors in human endothelial cells. Naunyn Schmiedebergs Arch Pharmacol. 1991;344:126-129.
25. Mülsch A, Bassenge E, Busse R. Nitric oxide synthesis in endotheliai cytosol: evidence for a calcium-dependent and a calcium-independent mechanism. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:767-770.
26. Fleming I, Hecker M, Busse R. Intracellular alkalinization induced by bradykinin sustains activation of the constitutive nitric oxide synthase in endothelial cells. Circ Res. 1994;74:1220-1226.
27. Fleming I, Fisslthaler B, Busse R. Calcium signaling in endothelial cells involves activation of tyrosine kinases and leads to activation of mitogen-activated protein kinases. Circ Res. 1995;76:522-529.
28. Davis PD, Hill CH, Keech E, Lawton G, Nixon JS, Sedgwick AD, Wadsworth J, Westmacott D, Wilkinson SE. Potent selective inhibitors of protein kinase C. FEBS Lett. 1989;259:61-63.
29. 2+. Am J Physiol. 1995;269:C863-C869.
30. Kanai AJ, Strauss HC, Truskey GA, Crews AL, Grunfeld S, Malinski T. Shear stress induces ATP-independent transient nitric oxide release from vascular endothelial cells, measured directly with a porphyrinic microsensor. Circ Res. 1995;77:284-293.
31. Kuchan MJ, Frangos JA. Role of calcium and calmodulin in flow-induced nitric oxide production in endothelial cells. Am J Physiol. 1994;266:C628-C636.
32. Barbee KA, Davies PF, Lal R. Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy. Circ Res. 1994;74:163-171.
33. Ziegelstein RC, Cheng L, Capogrossi MC. Flow-dependent cytosolic acidification of vascular endothelial cells. Science. 1992; 258:656-659.
34. Mobley PL, Hedberg K, Bonin L, Chen B, Griffith OH. Decreased phosphorylation of four 20-kDa proteins precedes staurosporine-induced disruption of the actin/myosin cytoskeleton in rat astrocytes. Exp Cell Res. 1994;214:55-66.
35. Schlaepfer DD, Hanks SK, Hunter T, van der Geer P. Integrin-mediated signal transduction linked to Ras pathway binding to focal adhesion kinase. Nature. 1994;372:786-791.
36. Fleming I, Bara AT, Busse R. Calcium signalling and autacoid production in endothelial cells are modulated by changes in tyrosine kinase and phosphatase activity. J Vasc Res. In press.
37. Tseng H, Peterson TE, Berk BC. Fluid shear stress stimulates mitogen-activated protein kinase in endothelial cells. Circ Res. 1995;77:869-878.