Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase

European Journal of Biochemistry

Volumn 270, Issue 5, 2003, Pages 971-980

  Trippe, Ralf ^a   Richly, Holger ^a,b   Benecke, Bernd Joachim ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

3 uridylation; Affinity chromatography; Terminal uridylyltransferase; U6 snRNA

Indexed keywords

HEXOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; MAGNESIUM CHLORIDE; NUCLEOTIDE; RNA POLYMERASE; SMALL NUCLEAR RNA; URIDINE PHOSPHATE; URIDINE TRIPHOSPHATE;

ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBSTRATE; GEL MOBILITY SHIFT ASSAY; GENE MUTATION; GENE STRUCTURE; GENETIC TRANSCRIPTION; HELA CELL; HUMAN; HUMAN CELL; IONIC STRENGTH; MOLECULAR WEIGHT; MOLECULE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REACTION ANALYSIS; RNA SYNTHESIS;

BASE SEQUENCE; DNA PRIMERS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RNA NUCLEOTIDYLTRANSFERASES; RNA, SMALL NUCLEAR;

MAMMALIA;

EID: 0037335898     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03466.x     Document Type: Article

References (31)

1. Will, C.L. & Lührmann, R. (1997) Protein functions in pre-mRNA splicing. Curr. Opin. Cell Biol. 9, 320-328.
2. Burge, C.B., Tuschl, T.H. & Sharp, P.A. (1999) Splicing of precursors to mRNAs by the spliceosomes. In RNA World II (Gesteland, R.F., Cech, T.R. & Atkins, J.F., eds), pp. 525-560. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
3. Nilsen, T.W. (1998) RNA-RNA interactions in nuclear pre-mRNA splicing. In RNA Structure and Function (Simons, R. & Grunberg-Manago, M., eds), pp. 279-307. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
4. Staley, J.P. & Guthrie, C. (1998) Mechanical devices of the spliceosome: motors, clocks, springs and things. Cell 92, 315-326.
5. Kuhn, A.N., Li, Z. & Brow, D.A. (1999) Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome. Mol. Cell 3, 65-75.
6. Kuhn, A.N. & Brow, D.A. (2000) Suppressors of cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation. Genetics 155, 1667-1682.
7. Kunkel, G.R., Maser, R.L., Clavet, J.P. & Pederson, T. (1986) U6 small nuclear RNA is transcribed by RNA polymerase III. Proc. Natl Acad. Sci. USA 83, 8575-8579.
8. Reddy, R., Henning, D., Das, G., Harless, M. & Wright, D. (1987) The capped U6 small nuclear RNA is transcribed by RNA polymerase III. J. Biol. Chem. 262, 75-81.
9. Singh, R. & Reddy, R. (1989) α-Monomethyl-phosphate: a cap structure in spliceosomal U6 snRNA. Proc. Natl Acad. Sci. USA 86, 8280-8283.
10. Brow, D.A. & Guthrie, C. (1988) Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature (London) 334, 213-218.
11. Branlant, C., Krol, A., Ebel, J.P., Lazar, E., Haendler, B. & Jacob, M. (1982) U2 RNA shares a structural domain with U1, U4 and U5 RNAs. EMBO J. 1, 1259-1265.
12. Liautard, J.P., Sri-Widada, J., Brunal, C. & Jeanteur, P. (1982) Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs. J. Mol. Biol. 162, 623-643.
13. Cooper, M., Jonston, L.H. & Beggs, J.D. (1995) Identification and characterization of Uss1p (Sdb23p): a novel U6 snRNA associated protein with significant similarity to core proteins of small nuclear ribonucleoproteins. EMBO J. 14, 2066-2075.
14. Séraphin, B. (1995) Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs. EMBO J. 14, 2089-2098.
15. Achsel, T., Brahms, H., Kastner, B., Bachi, A., Wilm, M. & Lührmann, R. (1999) A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. EMBO J. 18, 5789-5802.
16. Mayes, A.E., Verdone, L., Legrain, P. & Beggs, J.D. (1999) Characterization of Sm-like proteins in yeast and their association with U6 snRNA. EMBO J. 18, 4321-4331.
17. Lund, E. & Dahlberg, J.E. (1992) Cyclic 2′,3′-phosphates and nontemplated nucleotides at the 3′ end of spliceosomal U6 small nuclear RNAs. Science 255, 327-330.
18. Rinke, J. & Steitz, J.A. (1985) Association of the lupus antigen La with a subset of U6 snRNA molecules. Nucleic Acids Res. 13, 2617-2629.
19. Gu, J., Shumyatsky, G., Makan, N. & Reddy, R. (1997) Formation of 2′,3′-cyclic phosphates at the 3′end of human U6 small nuclear RNA in vitro. Identification of 2′,3′-cyclic phosphates at the 3′ ends of human signal recognition particle and mitochondrial RNA processing RNAs. J. Biol. Chem. 272, 21989-21993.
20. Booth, B.L. & Pugh, B.F. (1997) Identification and characterization of a nuclease specific for the 3′ end of the U6 small nuclear RNA. J. Biol. Chem. 272, 984-991.
21. Trippe, R., Sandrock, B. & Benecke, B.J. (1998) A highly specific terminal uridylyl transferase modifies the 3′-end of U6 small nuclear RNA. Nucleic Acids Res. 26, 3119-3126.
22. Weil, P.A., Segall, J., Harris, B., Ng, S.-Y. & Roeder, R.G. (1979) Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates. J. Biol. Chem. 254, 6163-6173.
23. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
24. Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. & Green, M.R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
25. Fortner, D.M., Troy, R.G. & Brow, D.A. (1994) A stem/loop in U6 RNA defines a conformational switch required for pre mRNA splicing. Genes Dev. 8, 221-233.
26. Zabel, P., Dorssers, L., Wernars, K. & VanKammen, A. (1981) Terminal uridylyl transferase of Vigna unguiculata: purification and characterization of an enzyme catalyzing the addition of a single UMP residue to the 3′-end of an RNA primer. Nucleic Acids Res. 11, 2433-2453.
27. Chen, Y., Sinha, K., Perumal, K. & Reddy, R. (2000) Effect of 3′ terminal adenylic acid residue on the uridylation of human small RNAs in vitro and in frog oocytes. RNA 6, 1277-1288.
28. Andrews, N.C., Levin, D. & Baltimore, D. (1985) Poliovirus replicase stimulation by terminal uridylyl transferase. J. Biol. Chem. 260, 7628-7635.
29. Tazi, J., Forne, T., Janteur, P., Cathala, G. & Brunel, C. (1993) Mammalian U6 small nuclear RNA undergoes 3′ end modifications within the spliceosome. Mol. Cell. Biol. 13, 1641-1650.
30. Aphasizhev, R., Sbicego, S., Peris, M., Jang, S.-H., Aphasizheva, I., Simpson, A.M., Rivlin, A. & Simpson, L. (2002) Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing. Cell 108, 537-648.
31. Perumal, K., Sinha, K., Henning, D. & Reddy, R. (2001) Purification, characterization and cloning of the cDNA of human SRP RNA-3′ adenylating enzyme. J. Biol. Chem. 276, 21791-21796.