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Volumn 14, Issue 1, 2003, Pages 101-109

Stable isotope-coded proteomic mass spectrometry

Author keywords

[No Author keywords available]

Indexed keywords

2 METHYLOXY 4,5 DIHYDRO 1H IMIDAZOLE; AVIDIN; BIOLOGICAL MARKER; BROMOACETIC ACID; CAMPTOTHECIN; CARBON; CARBON 13; MESSENGER RNA; PROTEIN TYROSINE KINASE; REAGENT; RECOMBINANT PROTEIN; STABLE ISOTOPE; SUCCINIC ANHYDRIDE; UNCLASSIFIED DRUG;

EID: 0037307837     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(02)00014-9     Document Type: Review
Times cited : (174)

References (58)
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    • Phosphoprotein isotope-coded affinity tag approach for isolating and quantitating phosphopeptides in proteome-wide analyses
    • This paper describes the first experimental report of an isotope-coding approach in conjunction with an affinity tag to enrich and quantify phosphoproteins. A PhIAT reagent, containing a nucleophilic sulfhydryl and an isotopic label covalently linked to a biotin moiety, was synthesized and could be used to reduce the pSer and pThr derivatization into a one step process.
    • Goshe M.B., Conrads T.P., Panisko E.A., Angell N.H., Veenstra T.D., Smith R.D. Phosphoprotein isotope-coded affinity tag approach for isolating and quantitating phosphopeptides in proteome-wide analyses. Anal. Chem. 73:2001;2578-2586 This paper describes the first experimental report of an isotope-coding approach in conjunction with an affinity tag to enrich and quantify phosphoproteins. A PhIAT reagent, containing a nucleophilic sulfhydryl and an isotopic label covalently linked to a biotin moiety, was synthesized and could be used to reduce the pSer and pThr derivatization into a one step process.
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    • Phosphoprotein isotope-coded affinity tags: Application to the enrichment and identification of low-abundance phosphoproteins
    • This work extended the PhIAT approach to using μLC-MS/MS to sequence phosphopeptides and identify the sites(s) of phosphorylation for low-level phosphoproteins. Details involving the quantitation of phosphorylation state using PhIAT were also discussed.
    • Goshe M.B., Veenstra T.D., Panisko E.A., Conrads T.P., Angell N.H., Smith R.D. Phosphoprotein isotope-coded affinity tags: application to the enrichment and identification of low-abundance phosphoproteins. Anal. Chem. 74:2002;607-616 This work extended the PhIAT approach to using μLC-MS/MS to sequence phosphopeptides and identify the sites(s) of phosphorylation for low-level phosphoproteins. Details involving the quantitation of phosphorylation state using PhIAT were also discussed.
    • (2002) Anal. Chem. , vol.74 , pp. 607-616
    • Goshe, M.B.1    Veenstra, T.D.2    Panisko, E.A.3    Conrads, T.P.4    Angell, N.H.5    Smith, R.D.6
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    • Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome
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    • Oda, Y.1    Nagasu, T.2    Chait, B.T.3
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    • A systematic approach to the analysis of protein phosphorylation
    • 2H atoms would enable differences in relative phosphopeptide abundance to be quantified.
    • 2H atoms would enable differences in relative phosphopeptide abundance to be quantified.
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    • Zhou, H.1    Watts, J.D.2    Aebersold, R.3
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    • Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae
    • This article describes an elegant method of 'phosphoprofiling' using esterification of the carboxylate groups of peptides and IMAC to enhance detection and identification of phosphopeptides when coupled to μLC-MS/MS. Although not experimentally demonstrated, the authors mention that comparative proteomics could be performed by differentially esterifying the carboxylic acid groups using methanol (control) and deuterated methanol (experimental).
    • Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat. Biotechnol. 20:2002;301-305 This article describes an elegant method of 'phosphoprofiling' using esterification of the carboxylate groups of peptides and IMAC to enhance detection and identification of phosphopeptides when coupled to μLC-MS/MS. Although not experimentally demonstrated, the authors mention that comparative proteomics could be performed by differentially esterifying the carboxylic acid groups using methanol (control) and deuterated methanol (experimental).
    • (2002) Nat. Biotechnol. , vol.20 , pp. 301-305
    • Ficarro, S.B.1    McCleland, M.L.2    Stukenberg, P.T.3    Burke, D.J.4    Ross, M.M.5    Shabanowitz, J.6    Hunt, D.F.7    White, F.M.8


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.